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Flowcytometric Determination of T-lymphocyte Expression of CD4+ and CD8+ Cells in Systemic Lupus Erythematosus


Affiliations
1 Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt
     

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The Purpose: Flow cytometric analysis to quantify the T-lymphocyte expression of CD4+ and CD8+ cells. Patients and Method: This study was conducted along the period from March 2018 to March 2019 on patients diagnosed as having systemic lupus erythematosus. The patients were selected from those who attended Rheumatology and Nephrology outpatient clinics, Minia University Hospital, Egypt. Group I: It consists of 40 apparently healthy women to serve as a control group. Group II: It comprised 40 SLE women. Results: The area under ROC curve of lymphocyte expression of CD4+ and CD8+ were 0.815 (95% CI= 0.712-0.893, p<0.001), at a cutoff value of < 32% and 0.675 (95% CI= 0.561-0.776, p=0.004) at a cutoff value of < 18.8, respectively. CD4 lymphocyte expression exhibited sensitivity, specificity, PPV, NPV, and accuracy of 77.5%, 87.5%, 86.1%, 79.5% and 82.5%, respectively, whereas those of CD8 were 32.5%, 100%, 100%, 59.7% and 66.2%, respectively. Conclusion: We provided more scientific insights into the flow cytometric analysis to quantify the T-lymphocyte expression of CD4+ and CD8+ cells.


Keywords

SLE; CD4; CD8
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  • Flowcytometric Determination of T-lymphocyte Expression of CD4+ and CD8+ Cells in Systemic Lupus Erythematosus

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Authors

Nehal Ibrahim Abbas
Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt
Ashraf Mohamed Osman
Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt
Amal Mahmoud Kamal El-Din
Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt
Emad Allam Abdel-Naem
Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt
Manal Mohamed Saber
Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt

Abstract


The Purpose: Flow cytometric analysis to quantify the T-lymphocyte expression of CD4+ and CD8+ cells. Patients and Method: This study was conducted along the period from March 2018 to March 2019 on patients diagnosed as having systemic lupus erythematosus. The patients were selected from those who attended Rheumatology and Nephrology outpatient clinics, Minia University Hospital, Egypt. Group I: It consists of 40 apparently healthy women to serve as a control group. Group II: It comprised 40 SLE women. Results: The area under ROC curve of lymphocyte expression of CD4+ and CD8+ were 0.815 (95% CI= 0.712-0.893, p<0.001), at a cutoff value of < 32% and 0.675 (95% CI= 0.561-0.776, p=0.004) at a cutoff value of < 18.8, respectively. CD4 lymphocyte expression exhibited sensitivity, specificity, PPV, NPV, and accuracy of 77.5%, 87.5%, 86.1%, 79.5% and 82.5%, respectively, whereas those of CD8 were 32.5%, 100%, 100%, 59.7% and 66.2%, respectively. Conclusion: We provided more scientific insights into the flow cytometric analysis to quantify the T-lymphocyte expression of CD4+ and CD8+ cells.


Keywords


SLE; CD4; CD8



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F195047