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Molecular Sequencing Study of Salmonella spp Which Isolated from Cattle Meat and Human Stool


Affiliations
1 Lecturer, AL-Qadisiyah University- College of Biotechnology, Iraq
2 Assistance Professor, AL-Qadisiyah University- College of Biotechnology, Iraq
3 Assistance Lecturer, AL-Qadisiyah University- Veterinary Medicine, Iraq
     

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Aim of our study is investigation of the genetic relationships between Salmonella spp isolates which taken from human stool isolates and Salmonella spp isolates which taken from cattle meat. The total number of study isolates is one hundred (50 cattlebeef and 50human stools. Bacterial isolation and bacterial identification are used for testing the samples for culturing and examined by biochemical tests then detected by using 16S rRNA gene in PCR that designed by NCBI and make DNA sequencing for two positive isolates. Our results revealed that percentage of Salmonellaspp was 8/50(16%) which isolated from cattle beef and was 10/50(20%) which isolated from human stool. The PCR was demonstrated highly specific and sensitive. DNA sequencing of part of 16S rRNA was show highly similarity with NCBI-Blast data and the phylogenetic study was reveal genetic homology at (0.5 genetic change) between animals and human isolates and then the gene sequence deposited into NCBI-Genbank accession numbers accession numbers (MN523665.1) and (MG388337.1). In conclusion, our study is done for first-time in Iraq of the genetic correlating between Salmonellaspp which isolated from cattle meat and human stool. So, it is important to know the cattle meat is significant source for spreading infection between human and animals.

Keywords

Sequencing, Salmonellaspp, Cattle, Human stool.
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  • Molecular Sequencing Study of Salmonella spp Which Isolated from Cattle Meat and Human Stool

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Authors

Hiba Shehab Ahmed
Lecturer, AL-Qadisiyah University- College of Biotechnology, Iraq
Wafaaabdalwahed Jheel
Assistance Professor, AL-Qadisiyah University- College of Biotechnology, Iraq
Saba Falah Klaif
Assistance Lecturer, AL-Qadisiyah University- Veterinary Medicine, Iraq

Abstract


Aim of our study is investigation of the genetic relationships between Salmonella spp isolates which taken from human stool isolates and Salmonella spp isolates which taken from cattle meat. The total number of study isolates is one hundred (50 cattlebeef and 50human stools. Bacterial isolation and bacterial identification are used for testing the samples for culturing and examined by biochemical tests then detected by using 16S rRNA gene in PCR that designed by NCBI and make DNA sequencing for two positive isolates. Our results revealed that percentage of Salmonellaspp was 8/50(16%) which isolated from cattle beef and was 10/50(20%) which isolated from human stool. The PCR was demonstrated highly specific and sensitive. DNA sequencing of part of 16S rRNA was show highly similarity with NCBI-Blast data and the phylogenetic study was reveal genetic homology at (0.5 genetic change) between animals and human isolates and then the gene sequence deposited into NCBI-Genbank accession numbers accession numbers (MN523665.1) and (MG388337.1). In conclusion, our study is done for first-time in Iraq of the genetic correlating between Salmonellaspp which isolated from cattle meat and human stool. So, it is important to know the cattle meat is significant source for spreading infection between human and animals.

Keywords


Sequencing, Salmonellaspp, Cattle, Human stool.



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F195044