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Antibacterial Activity of Sappan Wood (Caesalpinia Sappan L.) against Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis


Affiliations
1 Department of Oral Biology, Universitas Airlangga, Surabaya, Indonesia
2 Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
     

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The aim of this study was to find the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of sappan wood ethanol extract (Caesalpinia sappan L.) toward the growth of A. actinomycetemcomitans and P. gingivalis. The randomized posttest only control group design was applied to this study. The sappan wood ethanol extract was conducted with maceration method using 96% ethanol. Diluted to 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, and 0.39% concentration. The MIC and MBC values of sappan wood ethanol extract toward A. actinomycetemcomitans and P. gingivalis was then known via the evaluation for Colony Forming Units (CFUs) in MH medium. The MIC and MBC of sappan wood ethanol extract toward A. actinomycetemcomitans and P. gingivalis was at 1.56% and 3.125% concentration. The ethanol extract of sappan wood reduces the number of bacterial colonies significantly at p=0.00. This study concluded that the growth of A. actinomycetemcomitans and P. gingivalis can be inhibited by sappan wood ethanol extract (Caesalpinia sappan L.) at MIC 1.56% and MBC 3.125% concentration.

Keywords

Sappan Wood Ethanol Extract, MIC, MBC, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis
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  • Antibacterial Activity of Sappan Wood (Caesalpinia Sappan L.) against Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis

Abstract Views: 98  |  PDF Views: 0

Authors

Hendrik Setia Budi
Department of Oral Biology, Universitas Airlangga, Surabaya, Indonesia
Pratiwi Soesilowati
Department of Oral Biology, Universitas Airlangga, Surabaya, Indonesia
Mitha Jati Wirasti
Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Abstract


The aim of this study was to find the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of sappan wood ethanol extract (Caesalpinia sappan L.) toward the growth of A. actinomycetemcomitans and P. gingivalis. The randomized posttest only control group design was applied to this study. The sappan wood ethanol extract was conducted with maceration method using 96% ethanol. Diluted to 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, and 0.39% concentration. The MIC and MBC values of sappan wood ethanol extract toward A. actinomycetemcomitans and P. gingivalis was then known via the evaluation for Colony Forming Units (CFUs) in MH medium. The MIC and MBC of sappan wood ethanol extract toward A. actinomycetemcomitans and P. gingivalis was at 1.56% and 3.125% concentration. The ethanol extract of sappan wood reduces the number of bacterial colonies significantly at p=0.00. This study concluded that the growth of A. actinomycetemcomitans and P. gingivalis can be inhibited by sappan wood ethanol extract (Caesalpinia sappan L.) at MIC 1.56% and MBC 3.125% concentration.

Keywords


Sappan Wood Ethanol Extract, MIC, MBC, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F194988