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Ethanol Extract with Black Cumin (Nigella Sativa) Against sFlt-1 Level and VEGF Serum on Laboratory Mice with Preeclampsia


Affiliations
1 Bangkalan Midwifery Diploma Study Program, the Health Ministry Polytechnic of Surabaya, Indonesia
2 Master Program in Biomedical Sciences, Brawijaya University, Indonesia
3 Soetomo Midwifery Diploma Study Program, the Health Ministry Polytechnic of Surabaya, Indonesia
4 Faculty of Nursing, Universitas Airlangga, Indonesia
     

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Introduction: Preeclampsia is one of the complications that occur in pregnancies. This study was aimed to study the factors that affect the of giving ethanol extract with black cumin (Nigella sativa) against sFlt-1 level and VEGF serum on laboratory mice induced preeclampsia. Method: Laboratory experimental research with posttest only control group design. This study used 30 BALB/C laboratory mice, divided into 6 groups, namely negative controls: pregnant mice injected serum from normal pregnant women, positive controls; mice modeled preeclampsia, and treatment groups 1, 2, 3 and 4 are preeclampsia mice received a dose of 500 mg, 1000 mg, 1500 mg and 2000 mg/kg weight of Nigella sativa ethanol extract for 5 days. Statistical analysis using ANOVA Result: The mean serum sFlt-1 level in mice modeled preeclampsia and treatment group dose 500mg, 1000mg, 1500mg and 2000mg (2510.3+182.2 pg/mL, 2142.5+171.9 pg/mL, 1309+161.3 p/mL, and 1500+169.9, respectively) pg/mL) showed a significant difference (p<0.05) and found a decrease in serum sFlt-1 levels with increasing doses. The mean serum VEGF levels in preeclampsia mice and treatment groups were 500 mg, 1000 mg, 1500 mg and 2000 mg (50.25±2.85b pg/mL, 60.18±4.81c pg/mL respectively, 71.89±2.38d pg/mL, 66.51±1.87 e pg/mL) showed a significant difference (p<0.05) and found an increase in serum VEGF levels as the dose increased. Conclusion: Giving of Black Cumin extract (Nigella sativa) decreases serum sFlt-1 levels and increases serum VEGF levels in preeclampsia mice model and the effect is dependent dose.

Keywords

sFlt-1, VEGF, Nigella sativa, preeclampsia
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  • Ethanol Extract with Black Cumin (Nigella Sativa) Against sFlt-1 Level and VEGF Serum on Laboratory Mice with Preeclampsia

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Authors

Deasy Irawati
Bangkalan Midwifery Diploma Study Program, the Health Ministry Polytechnic of Surabaya, Indonesia
Hidayat Suyuti
Master Program in Biomedical Sciences, Brawijaya University, Indonesia
Titi Maharrani
Soetomo Midwifery Diploma Study Program, the Health Ministry Polytechnic of Surabaya, Indonesia
Fitriah
Bangkalan Midwifery Diploma Study Program, the Health Ministry Polytechnic of Surabaya, Indonesia
Ani Media Harrumi
Soetomo Midwifery Diploma Study Program, the Health Ministry Polytechnic of Surabaya, Indonesia
Suryaningsih
Bangkalan Midwifery Diploma Study Program, the Health Ministry Polytechnic of Surabaya, Indonesia
Nursalam
Faculty of Nursing, Universitas Airlangga, Indonesia

Abstract


Introduction: Preeclampsia is one of the complications that occur in pregnancies. This study was aimed to study the factors that affect the of giving ethanol extract with black cumin (Nigella sativa) against sFlt-1 level and VEGF serum on laboratory mice induced preeclampsia. Method: Laboratory experimental research with posttest only control group design. This study used 30 BALB/C laboratory mice, divided into 6 groups, namely negative controls: pregnant mice injected serum from normal pregnant women, positive controls; mice modeled preeclampsia, and treatment groups 1, 2, 3 and 4 are preeclampsia mice received a dose of 500 mg, 1000 mg, 1500 mg and 2000 mg/kg weight of Nigella sativa ethanol extract for 5 days. Statistical analysis using ANOVA Result: The mean serum sFlt-1 level in mice modeled preeclampsia and treatment group dose 500mg, 1000mg, 1500mg and 2000mg (2510.3+182.2 pg/mL, 2142.5+171.9 pg/mL, 1309+161.3 p/mL, and 1500+169.9, respectively) pg/mL) showed a significant difference (p<0.05) and found a decrease in serum sFlt-1 levels with increasing doses. The mean serum VEGF levels in preeclampsia mice and treatment groups were 500 mg, 1000 mg, 1500 mg and 2000 mg (50.25±2.85b pg/mL, 60.18±4.81c pg/mL respectively, 71.89±2.38d pg/mL, 66.51±1.87 e pg/mL) showed a significant difference (p<0.05) and found an increase in serum VEGF levels as the dose increased. Conclusion: Giving of Black Cumin extract (Nigella sativa) decreases serum sFlt-1 levels and increases serum VEGF levels in preeclampsia mice model and the effect is dependent dose.

Keywords


sFlt-1, VEGF, Nigella sativa, preeclampsia



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F194966