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Correlation between Bio-Film Formation and Carbapenem Resistant Enterobacteriaceae Isolates Obtained from Various Clinical Specimens


Affiliations
1 B.Sc. M.Sc. Medical Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana Ambala, Haryana, India
2 Professor, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana Ambala, Haryana, India
3 Assistant Professor, Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, 31982, Saudi Arabia
4 Assistant Professor, Department of Microbiology, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana Ambala, Haryana, India
       

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Introduction: The ability to form bio-film is a universal trait of bacteria by attaching to the surfaces. Carbapenem resistance in Enterobacteriaceae might be endorsed by bio-film formation which augmented colonization of pathogens. Aim: To correlate between bio-film formation and carbapenem resistant Enterobacteriaceae isolates obtained from various clinical specimens and to compare the qualitative and quantitative assay for bio-film production. Method: Study was conducted on 150 strains of Enterobacteriaceae isolates. of these, carbapenem resistant Enterobacteriaceae isolates were subjected for bio-film formation by Modified Congo red agar method, tube adherence method and Tissue culture plate method. Results: Carbapenem resistant Enterobacteriaceae strains were found to be 60.66% and the rate of biofilm producers was 75.53% by any of the phenotypic method. Tissue culture plate method was found to be (67.6%) better than Modified Congo Red agar method (54.9%) and Tube adherence method (39.4%). The highest number of bio-film producers was isolated from urinary tract infections (36.61%). Conclusion: TCP method is most reliable, precise and sensitive method for detection of bio-film formation by Enterobacteriaceae isolates and is ideal to use as a general screening tool to detect bio-film production.

Keywords

Carbapenem resistant Enterobacteriaceae; bio-film; qualitative; quantitative assays.
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  • Correlation between Bio-Film Formation and Carbapenem Resistant Enterobacteriaceae Isolates Obtained from Various Clinical Specimens

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Authors

Sakshi Dalal
B.Sc. M.Sc. Medical Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana Ambala, Haryana, India
Sonia Mehta
Professor, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana Ambala, Haryana, India
Shinu Pottathil
Assistant Professor, Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, 31982, Saudi Arabia
Varsha A. Singh
Professor, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana Ambala, Haryana, India
Shavi Nagpal
Assistant Professor, Department of Microbiology, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana Ambala, Haryana, India

Abstract


Introduction: The ability to form bio-film is a universal trait of bacteria by attaching to the surfaces. Carbapenem resistance in Enterobacteriaceae might be endorsed by bio-film formation which augmented colonization of pathogens. Aim: To correlate between bio-film formation and carbapenem resistant Enterobacteriaceae isolates obtained from various clinical specimens and to compare the qualitative and quantitative assay for bio-film production. Method: Study was conducted on 150 strains of Enterobacteriaceae isolates. of these, carbapenem resistant Enterobacteriaceae isolates were subjected for bio-film formation by Modified Congo red agar method, tube adherence method and Tissue culture plate method. Results: Carbapenem resistant Enterobacteriaceae strains were found to be 60.66% and the rate of biofilm producers was 75.53% by any of the phenotypic method. Tissue culture plate method was found to be (67.6%) better than Modified Congo Red agar method (54.9%) and Tube adherence method (39.4%). The highest number of bio-film producers was isolated from urinary tract infections (36.61%). Conclusion: TCP method is most reliable, precise and sensitive method for detection of bio-film formation by Enterobacteriaceae isolates and is ideal to use as a general screening tool to detect bio-film production.

Keywords


Carbapenem resistant Enterobacteriaceae; bio-film; qualitative; quantitative assays.



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F194920