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In Vitro Antibacterial and Anticancer Study of Bioactive Compounds Isolated from Punica Grantum Peel


Affiliations
1 Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, India
     

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Plants have been used as natural medicines since the dawn of human kind. This research was focused on phytochemical analysis of Punica grantum peel extract consist of ethanol: chloroform (99:1) as solvent and its constituents were molecularly characterized by GCMS analysis and its ability as an antioxidant, anticancer against K562 cell and antibacterial against some food borne pathogens which comprises of 5 gram-positive and 3-gram-negative bacteria. The phytochemical analysis was performed biochemically and results reveals the presence of secondary metabolites such as Flavonoids, Alkaloids, Tannins, Saponins, etc. and absence of glycosides. The extract was tested against both gram-positive and gram-negative bacteria by agar well diffusion method and its results have shown that the streptococcus faecalis have shown highest susceptibility against the extract. Its antioxidant potential was quantitatively determined by DPPH assay and results showed high range of antioxidant present in the extract with IC50 as 504.9μg/ml. The cytotoxicity assay was performed with peel extract of different concentration against K562 cancer lines which is a myelogenous leukemia cell line. However, the cytotoxicity is less when compared with cell viability and its effects were mild against the cancer cells.

Keywords

Punicagranatum, DPPH, GCMS, K562 Cancer Cells (Myelogenous Leukemia).
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  • In Vitro Antibacterial and Anticancer Study of Bioactive Compounds Isolated from Punica Grantum Peel

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Authors

Arunava Das
Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, India
J. Bindhu
Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, India
M. Bharath
Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, India

Abstract


Plants have been used as natural medicines since the dawn of human kind. This research was focused on phytochemical analysis of Punica grantum peel extract consist of ethanol: chloroform (99:1) as solvent and its constituents were molecularly characterized by GCMS analysis and its ability as an antioxidant, anticancer against K562 cell and antibacterial against some food borne pathogens which comprises of 5 gram-positive and 3-gram-negative bacteria. The phytochemical analysis was performed biochemically and results reveals the presence of secondary metabolites such as Flavonoids, Alkaloids, Tannins, Saponins, etc. and absence of glycosides. The extract was tested against both gram-positive and gram-negative bacteria by agar well diffusion method and its results have shown that the streptococcus faecalis have shown highest susceptibility against the extract. Its antioxidant potential was quantitatively determined by DPPH assay and results showed high range of antioxidant present in the extract with IC50 as 504.9μg/ml. The cytotoxicity assay was performed with peel extract of different concentration against K562 cancer lines which is a myelogenous leukemia cell line. However, the cytotoxicity is less when compared with cell viability and its effects were mild against the cancer cells.

Keywords


Punicagranatum, DPPH, GCMS, K562 Cancer Cells (Myelogenous Leukemia).



DOI: https://doi.org/10.37506/v11%2Fi1%2F2020%2Fijphrd%2F193782