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Whole Cell Vaccine Vibrio Alginoliticus with Microcapsul Particle Alginate Process to Improve Cantang Grouper’s Life Epinephelus Fuscoguttatus-Lanceolatus


Affiliations
1 Biotechnology of Fisheres and Marine, Pasca Sarjana, Airlangga University, Indonesia
2 Department of Parasitology, Center for Marine Aquaculture Research and Fisheries Extension, Indonesia
3 Department of Parasitology, Airlangga University,, Indonesia
4 Department of Fish Health, Airlangga University, Indonesia
     

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Grouper cultivation is still constrained by the availability of seeds due to diseases caused by bacteria. Vibriosis is a disease caused by bacterial infections of the Vibrio genus such as Vibrio alginolyticus and Vibrio parahaemolyticus which are the most common in hatcheries. Whole Cell Vaccine (WCV) contains endotoxin components as antigens to stimulate antibody formation, including lipopolysaccharide from the cell wall of gram-negative bacteria. There is a problem of oral vaccination which can damagewhen it enters the low pH digestive system. Therefore, it is necessary to conduct a research to find out the ability of the Whole cell vaccine (WCV) of Vibrio alginoliticus bacteria by microencapsulation method of alginate gel as an oral alginate particle vaccine Vibrio alginoliticus to increase the survival of the grouper seeds of Epinephelus sp. This study used four types of treatment, namely control (-) without vaccine, control (+) using alginate only, dosage treatment 0.025 mg/5gr BB fish/day and 0.05 mg/5gr BB fish/day. The results of the Whole Cell Vaccine using alginate gel microcapsules process can increase the life range of cantang grouper seeds by 80% at 0.05 mg/5gr BB fish/day and 73% at 0.025 mg/5gr BB fish/day.

Keywords

Vibrio Alginolyticus, Whole Cell Vaccine, Alginate, Grouper, Vibriosis.
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  • Whole Cell Vaccine Vibrio Alginoliticus with Microcapsul Particle Alginate Process to Improve Cantang Grouper’s Life Epinephelus Fuscoguttatus-Lanceolatus

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Authors

Franch Dalahi
Biotechnology of Fisheres and Marine, Pasca Sarjana, Airlangga University, Indonesia
Silvia Yuhana
Biotechnology of Fisheres and Marine, Pasca Sarjana, Airlangga University, Indonesia
Ketut Mahardika
Department of Parasitology, Center for Marine Aquaculture Research and Fisheries Extension, Indonesia
Zafran
Department of Parasitology, Center for Marine Aquaculture Research and Fisheries Extension, Indonesia
Indah Mastuti
Department of Parasitology, Center for Marine Aquaculture Research and Fisheries Extension, Indonesia
Mufasirin
Department of Parasitology, Airlangga University,, Indonesia
Hari Suprapto
Department of Fish Health, Airlangga University, Indonesia

Abstract


Grouper cultivation is still constrained by the availability of seeds due to diseases caused by bacteria. Vibriosis is a disease caused by bacterial infections of the Vibrio genus such as Vibrio alginolyticus and Vibrio parahaemolyticus which are the most common in hatcheries. Whole Cell Vaccine (WCV) contains endotoxin components as antigens to stimulate antibody formation, including lipopolysaccharide from the cell wall of gram-negative bacteria. There is a problem of oral vaccination which can damagewhen it enters the low pH digestive system. Therefore, it is necessary to conduct a research to find out the ability of the Whole cell vaccine (WCV) of Vibrio alginoliticus bacteria by microencapsulation method of alginate gel as an oral alginate particle vaccine Vibrio alginoliticus to increase the survival of the grouper seeds of Epinephelus sp. This study used four types of treatment, namely control (-) without vaccine, control (+) using alginate only, dosage treatment 0.025 mg/5gr BB fish/day and 0.05 mg/5gr BB fish/day. The results of the Whole Cell Vaccine using alginate gel microcapsules process can increase the life range of cantang grouper seeds by 80% at 0.05 mg/5gr BB fish/day and 73% at 0.025 mg/5gr BB fish/day.

Keywords


Vibrio Alginolyticus, Whole Cell Vaccine, Alginate, Grouper, Vibriosis.



DOI: https://doi.org/10.37506/v10%2Fi12%2F2019%2Fijphrd%2F192110