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Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)


Affiliations
1 Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russian Federation
2 Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russian Federation
 

Testing systems for molecular identification of micropropagated elite aspen (Populus tremula L.) genotypes were developed on the base on microsatellite (SSR) loci. Out of 33 tested microsatellite loci, 14 were selected due to sustainable PCR amplification and substantial variability in elite clones of aspen aimed for establishment of fast-rotated forest plantations. All eight tested clones had different multilocus genotypes. Among 114 trees from three reference native stands located near the established plantations, 80 haplotypes were identified while some repeated genotypes were attributed to natural clones which appeared as a result of sprouting. The selected set of SSR markers showed reliable individual identification with low probability of appearance of identical aspen genotypes (a minimum of 4.8 ⋅ 10−10 and 1 × 10−4 for unrelated and related individuals, resp.). Case studies demonstrating practical applications of the test system are described including analysis of clonal structure and levels of genetic diversity in three natural aspen stands growing in the regions where plantations made of elite clones were established.
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  • Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

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Authors

Dmitry V. Politov
Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russian Federation
Maryana M. Belokon
Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russian Federation
Yuri S. Belokon
Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russian Federation
Tatyana A. Polyakova
Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russian Federation
Anna V. Shatokhina
Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russian Federation
Elena A. Mudrik
Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russian Federation
Anna B. Azarova
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russian Federation
Mikhail V. Filippov
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russian Federation
Konstantin A. Shestibratov
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russian Federation

Abstract


Testing systems for molecular identification of micropropagated elite aspen (Populus tremula L.) genotypes were developed on the base on microsatellite (SSR) loci. Out of 33 tested microsatellite loci, 14 were selected due to sustainable PCR amplification and substantial variability in elite clones of aspen aimed for establishment of fast-rotated forest plantations. All eight tested clones had different multilocus genotypes. Among 114 trees from three reference native stands located near the established plantations, 80 haplotypes were identified while some repeated genotypes were attributed to natural clones which appeared as a result of sprouting. The selected set of SSR markers showed reliable individual identification with low probability of appearance of identical aspen genotypes (a minimum of 4.8 ⋅ 10−10 and 1 × 10−4 for unrelated and related individuals, resp.). Case studies demonstrating practical applications of the test system are described including analysis of clonal structure and levels of genetic diversity in three natural aspen stands growing in the regions where plantations made of elite clones were established.