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Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil


Affiliations
1 Instituto de Quimica, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira 149, Bloco A, Cidade Universitaria, 21949-909 Rio de Janeiro, RJ, Brazil
2 Universidade do Estado do Rio do Janeiro (UERJ), Avenida Professor Manoel de Abreu 444, 2 andar, Vila Isabel, 20550-170 Rio de Janeiro, RJ, Brazil
3 Universidade do Estado do Rio de Janeiro (FFP-UERJ), Rua Dr. Francisco Portela 1470, Patronato, 24435-005 Sao Goncalo, RJ, Brazil
4 Escola de Quimica, Universidade Federal do Rio de Janeiro (UFRJ), Avenida Athos da Silveira 149, Bloco E, Cidade Universitaria, 21949-909 Rio de Janeiro, RJ, Brazil
 

The bacteriological quality of crabs from three different mangroves (Itaoca, Suruı, and Piedade) from Rio de Janeiro state, Brazil, was investigated using conventional and molecular methods. The results revealed high counts for total coliforms in meat and hepatopancreas samples. PCR analyses identified 25 Escherichia coli colonies in the Itaoca, Piedade, and Suruı samples, detecting 13 enterotoxigenic colonies and 9 enteroaggregative colonies. Respectively, 12, 11, and 21 Vibrio parahaemolyticus strains were detected in the Itaoca, Piedade, and Suru´ı samples. Two V. cholerae strains were detected in the Piedade samples.The E. coli strains isolated in the present study showed resistance to gentamicin. E. coli strains from the Piedade samples showed 33% resistance to chloramphenicol and the strains also showed multiresistance to several antimicrobial agents with a MAR index ranging from 0.12 to 0.31. Vibrio strains from Piedade, Itaoca, and Suruı showed 86%, 78%, and 85% resistance, respectively, to ampicillin. Theisolated Vibrio strains showed multiresistance to several antimicrobial agents, with a MAR index ranging from 0.12 to 0.25. The presence of these organisms in crab meat is an indication of microbial contamination, which may pose health risks to consumers when improperly cooked.
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  • Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil

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Authors

M. C. N. Carvalho
Instituto de Quimica, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira 149, Bloco A, Cidade Universitaria, 21949-909 Rio de Janeiro, RJ, Brazil
M. M. Jayme
Universidade do Estado do Rio do Janeiro (UERJ), Avenida Professor Manoel de Abreu 444, 2 andar, Vila Isabel, 20550-170 Rio de Janeiro, RJ, Brazil
G. S. Arenazio
Universidade do Estado do Rio de Janeiro (FFP-UERJ), Rua Dr. Francisco Portela 1470, Patronato, 24435-005 Sao Goncalo, RJ, Brazil
F. V. Araujo
Universidade do Estado do Rio de Janeiro (FFP-UERJ), Rua Dr. Francisco Portela 1470, Patronato, 24435-005 Sao Goncalo, RJ, Brazil
S. G. F. Leite
Escola de Quimica, Universidade Federal do Rio de Janeiro (UFRJ), Avenida Athos da Silveira 149, Bloco E, Cidade Universitaria, 21949-909 Rio de Janeiro, RJ, Brazil
E. M. Del Aguila
Instituto de Quimica, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira 149, Bloco A, Cidade Universitaria, 21949-909 Rio de Janeiro, RJ, Brazil

Abstract


The bacteriological quality of crabs from three different mangroves (Itaoca, Suruı, and Piedade) from Rio de Janeiro state, Brazil, was investigated using conventional and molecular methods. The results revealed high counts for total coliforms in meat and hepatopancreas samples. PCR analyses identified 25 Escherichia coli colonies in the Itaoca, Piedade, and Suruı samples, detecting 13 enterotoxigenic colonies and 9 enteroaggregative colonies. Respectively, 12, 11, and 21 Vibrio parahaemolyticus strains were detected in the Itaoca, Piedade, and Suru´ı samples. Two V. cholerae strains were detected in the Piedade samples.The E. coli strains isolated in the present study showed resistance to gentamicin. E. coli strains from the Piedade samples showed 33% resistance to chloramphenicol and the strains also showed multiresistance to several antimicrobial agents with a MAR index ranging from 0.12 to 0.31. Vibrio strains from Piedade, Itaoca, and Suruı showed 86%, 78%, and 85% resistance, respectively, to ampicillin. Theisolated Vibrio strains showed multiresistance to several antimicrobial agents, with a MAR index ranging from 0.12 to 0.25. The presence of these organisms in crab meat is an indication of microbial contamination, which may pose health risks to consumers when improperly cooked.