Indian Journal of Forensic Medicine & Toxicology

M. M. Al-Mahdawi ¹, M. K. Al-Mallah ², Al-Ne’ma Q. Sh. ²

Affiliations
1 College of Education for Pure Sciences, University of Diyala, IQ
2 Biotech. Lab., College of Education for Pure Sciences, University of Mosul, IQ

Abstract

In this investigation hairy ischolar_mains were induced on leave petioles of Ruta graveolens L. plants. These ischolar_mains were white in color and negative geotropism. Paper electrophoresis of hairy ischolar_mains extract resulted in the separation of black spots of agropine . These transformed ischolar_mains were cured from Agrobacterium rhizogenes, some cultures of these ischolar_mains were directly produced shoots, and other was orientated to produce callus. Subsequently this callus was capable to produce numerous regenerates. Both groups of shoots were ischolar_mained easily and successfully adapted to field conditions. Evidences of molecular biology assessment, including isolation and amplification(PCR) of DNA of each transformed tissues and plants coupled with DNA electrophoresis data proved the transfer of rol-genes, except rol B , in the regenerated plants. Conclusion: The genetic modified R. graveolens plants possess unique characters due to the conservation of rol-genes in their genomes.

Keywords

Ruta graveolens, Agrobacterium rhizogenes R1601, Agropine, PCR, rol-genes

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