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Pushpalatha, K. C.
- In-Vitro Cytotoxic Study of Moullava Spicata (Dalz.) Nicolson Leaf Extract
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Authors
Affiliations
1 Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri
2 Central Research Laboratory, A B Shetty Memorial Institute of Dental Sciences, Nitte University, IN
3 Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri, IN
1 Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri
2 Central Research Laboratory, A B Shetty Memorial Institute of Dental Sciences, Nitte University, IN
3 Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri, IN
Source
Indian Journal of Forensic Medicine & Toxicology, Vol 7, No 2 (2013), Pagination: 182-186Abstract
Objectives: The aim of present study is to evaluate cytotoxic and genotoxic activity of Moullava spicata (Dalz.) Nicolson.Materials and Method: The aqueous extract of this plant subjected to genotoxic and cytotoxic study using yeast viability test, hemolytic assay and Allium test.
Result: The extract showed dose dependent effect on yeast viability and hemolysis of erythrocytes. The Allium test showed significant reduction of ischolar_main length (p > 0.01) and number (p > 0.001) indicated cytotoxic effect of plant extract. Microscopic observations showed cytotoxic effects such as ghost cell, membrane damage or rupture, apoptotic bodies and the extent damage was increased significantly after 96 hours treatment.
Conclusion: The study showed that the leaf extract of the M. spicata contains various biologically active compounds and possess cytotoxic effects.
Keywords
Moullava spicata (Dalz.) Nicolson, Allium test, Hemolysis, Cytotoxicity
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