Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Sex Determination of in Vitro Produced Goat Embryos by Epididymal Spermatozoa Selected by Swim up Technique


Affiliations
1 Department of Surgery and Obstetrics, College of Veterinary Medicine/Baghdad University, Iraq
     

   Subscribe/Renew Journal


The study was conducted to determine the sex of the male and female goat embryos produced in vitro fertilization by Epididymal spermatozoa of local buck and separated by swim-up technique. The sex of produced embryo was identified by polymerase chain reaction (PCR). Results indicate that the fertilization by Epididymal spermatozoa selected using Swim-up technique at 200×g centrifugation showed that 48.70 ±0.62 % of spermatozoa were detected in the supernatant while the precipitate contained 42.1±0.70. Spermatozoa in the supernatant were used for IVF of matured oocytes. The sex of goat embryos produced in vitro fertilization was determined by polymerase chain reaction (PCR) using specific primers to detect the SRY gene. The percentage of male embryos recorded 71.73% while female embryos recorded only 28.26% from the total goat embryos obtained after IVF by sperms selected using swim-up at centrifugation force of 200×g. It was concluded that the use of swim-up technique up at centrifugation force of 200×g on Epididymal spermatozoa showed the ability of selection male embryos in caprine.

Keywords

Swim up, Gender, Goat, Cauda, Epididymis, Embryo, SRY Gene.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 81

PDF Views: 0




  • Sex Determination of in Vitro Produced Goat Embryos by Epididymal Spermatozoa Selected by Swim up Technique

Abstract Views: 81  |  PDF Views: 0

Authors

Sura Shakir Hammood
Department of Surgery and Obstetrics, College of Veterinary Medicine/Baghdad University, Iraq

Abstract


The study was conducted to determine the sex of the male and female goat embryos produced in vitro fertilization by Epididymal spermatozoa of local buck and separated by swim-up technique. The sex of produced embryo was identified by polymerase chain reaction (PCR). Results indicate that the fertilization by Epididymal spermatozoa selected using Swim-up technique at 200×g centrifugation showed that 48.70 ±0.62 % of spermatozoa were detected in the supernatant while the precipitate contained 42.1±0.70. Spermatozoa in the supernatant were used for IVF of matured oocytes. The sex of goat embryos produced in vitro fertilization was determined by polymerase chain reaction (PCR) using specific primers to detect the SRY gene. The percentage of male embryos recorded 71.73% while female embryos recorded only 28.26% from the total goat embryos obtained after IVF by sperms selected using swim-up at centrifugation force of 200×g. It was concluded that the use of swim-up technique up at centrifugation force of 200×g on Epididymal spermatozoa showed the ability of selection male embryos in caprine.

Keywords


Swim up, Gender, Goat, Cauda, Epididymis, Embryo, SRY Gene.



DOI: https://doi.org/10.37506/v14%2Fi1%2F2020%2Fijfmt%2F193088