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Thirteen New Yeast Strains Isolated from Cancer Patients in Basrah-Iraq by ITS rDNA Sequencing


Affiliations
1 Basrah University, College of Science, Ecology Department, Iraq
2 Southern Technical University, Basrah Technical Institute, Health community department, Iraq
3 Basrah University, College of Science, Biology Department, Iraq
     

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Yeast isolates were grown on CHR OMagar Candida medium to detect different types of the yeast species, and then the unidentified isolates were diagnosed by using molecular analysis of ITS region. From a total of 54 yeast isolates, 37 isolates appeared as different species of the genus Candida, the rest of species belong to Hanseniaspora, Kazachstania, Kluyveromyces, Magnusiomyces, Pichia and Saccharomyces. 13yeasts species in this study reported as new strains in Gen Bank, also species belong to C. pronicula, H. uvarum, K. exigua, K. marxianus, M. capitatus, Magnusiomyces sp., P. kudriavzevii, P. manshurica and S. bayanus x S. cerevisiae isolated for the first time in Iraq. We concluded that there are new emergent species of the yeasts inhabit the oral cavity of cancer patients undergoing chemotherapy, and the results showed that the molecular method provide a good approach for the identification.

Keywords

yeasts, cancer patient, molecular identification
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  • Thirteen New Yeast Strains Isolated from Cancer Patients in Basrah-Iraq by ITS rDNA Sequencing

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Authors

Mustafa A. Aldossary
Basrah University, College of Science, Ecology Department, Iraq
Bashar S. Abdulraheem
Southern Technical University, Basrah Technical Institute, Health community department, Iraq
Nasir A. Almansour
Basrah University, College of Science, Biology Department, Iraq
Munaff J. Abd Al-Abbas
Basrah University, College of Science, Biology Department, Iraq

Abstract


Yeast isolates were grown on CHR OMagar Candida medium to detect different types of the yeast species, and then the unidentified isolates were diagnosed by using molecular analysis of ITS region. From a total of 54 yeast isolates, 37 isolates appeared as different species of the genus Candida, the rest of species belong to Hanseniaspora, Kazachstania, Kluyveromyces, Magnusiomyces, Pichia and Saccharomyces. 13yeasts species in this study reported as new strains in Gen Bank, also species belong to C. pronicula, H. uvarum, K. exigua, K. marxianus, M. capitatus, Magnusiomyces sp., P. kudriavzevii, P. manshurica and S. bayanus x S. cerevisiae isolated for the first time in Iraq. We concluded that there are new emergent species of the yeasts inhabit the oral cavity of cancer patients undergoing chemotherapy, and the results showed that the molecular method provide a good approach for the identification.

Keywords


yeasts, cancer patient, molecular identification



DOI: https://doi.org/10.37506/v14%2Fi1%2F2020%2Fijfmt%2F193014