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Induction of Apoptosis in Myelogenous Leukemic K562 Cells by Ethanolic Leaf Extract of Annona muricata


Affiliations
1 Department of Biochemistry, University of Lagos, Akoka, Nigeria
2 Department of Cell biology, University of Lagos, Akoka, Nigeria
3 Department of Biochemistry, University of Port Harcourt, Rivers State, Nigeria
 

Prevalence of cancer, especially in Nigeria, is silently growing at exponential rates due to its nature and numerous risk factors associated with its development. Most chemotherapeutic drugs used today are expensive and toxic to normal cells, hence the need for alternative treatment options. In this present study, the efficacy of ethanolic extracts of Annona muricata leaves for its cytotoxicity potential and induction of apoptosis in K562 cancer cells, was investigated. Phytochemical screening verified presence of alkaloids, tannins, flavonoids, saponins, anthraquinones and cardiac glycosides. Using Neutral red uptake assay, the ethanolic extract showed peak cytotoxicity levels (PAnnona muricata induced apoptosis in the myelogenous leukemic K562 cell line. This supports the therapeutic application of Annona muricata to be considered as a natural product source for the development of pro-apoptotic drugs.

Keywords

Apoptosis, K562 Cells, Annona Muricata
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  • Induction of Apoptosis in Myelogenous Leukemic K562 Cells by Ethanolic Leaf Extract of Annona muricata

Abstract Views: 519  |  PDF Views: 448

Authors

A. U. Ezirim
Department of Biochemistry, University of Lagos, Akoka, Nigeria
V. I. Okachi
Department of Biochemistry, University of Lagos, Akoka, Nigeria
A. B. James
Department of Biochemistry, University of Lagos, Akoka, Nigeria
O. A. Adebeshi
Department of Cell biology, University of Lagos, Akoka, Nigeria
S. Ogunnowo
Department of Biochemistry, University of Lagos, Akoka, Nigeria
O. B. Odeghe
Department of Biochemistry, University of Port Harcourt, Rivers State, Nigeria

Abstract


Prevalence of cancer, especially in Nigeria, is silently growing at exponential rates due to its nature and numerous risk factors associated with its development. Most chemotherapeutic drugs used today are expensive and toxic to normal cells, hence the need for alternative treatment options. In this present study, the efficacy of ethanolic extracts of Annona muricata leaves for its cytotoxicity potential and induction of apoptosis in K562 cancer cells, was investigated. Phytochemical screening verified presence of alkaloids, tannins, flavonoids, saponins, anthraquinones and cardiac glycosides. Using Neutral red uptake assay, the ethanolic extract showed peak cytotoxicity levels (PAnnona muricata induced apoptosis in the myelogenous leukemic K562 cell line. This supports the therapeutic application of Annona muricata to be considered as a natural product source for the development of pro-apoptotic drugs.

Keywords


Apoptosis, K562 Cells, Annona Muricata

References