Open Access Open Access  Restricted Access Subscription Access

Microwave-Assisted Method for Simultaneous Hydrolysis and Extraction for Preparation of Geniposidic Acid from Eucommia ulmoides Bark using Basic Imidazolide Ionic Liquid


Affiliations
1 Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
2 College of Animal Science and Technology, Hebei North University, Zhangjiakou 075000, China
3 Landscape Architecture College, Northeast Forestry University, Harbin 150040, China
 

In order to hydrolyze geniposide and extract geniposidic acid, a novel microwave irradiation heating method for simultaneous hydrolysis and extraction for preparation of geniposidic acid from Eucommia ulmoides bark using basic imidazolide ionic liquid was developed in this work. Basic ionic liquid paired by imidazolide anion and 1-hexyl-3-methylimidazolium cation ([C6mim]Im) was used as a dual hydrolysis catalyst and extraction solvent in the proposed approach.The optimal concentration of [C6mim]Im was 0.4 mol/L. And the independent parameters optimized by Box-Behnken design (BBD) based on single-factor tests were as follows: microwave irradiation time of 20 min at power of 415Wand liquid-solid ratio of 15 mL/g. Yield of geniposidic acid was increased to 3.41±0.17 μmol/g. DM130macroporous resin, after reaction, was selected to separate geniposidic acid fromhydrolysate.The plant materials with different treatment were analyzed by scanning electron microscopy (SEM) and thermogravimetric analysis (TG). The analysis of SEM and TG further indicated that IMSHE is an efficient preparation method for geniposidic acid. It is reasonable to assume that the method and the dual hydrolysis catalyst and extraction solvent would offer an alternative for the preparation of some other kinds of bioactive substances from plant materials.
User
Notifications
Font Size

Abstract Views: 65

PDF Views: 1




  • Microwave-Assisted Method for Simultaneous Hydrolysis and Extraction for Preparation of Geniposidic Acid from Eucommia ulmoides Bark using Basic Imidazolide Ionic Liquid

Abstract Views: 65  |  PDF Views: 1

Authors

Lili Li
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
Yupin Guo
College of Animal Science and Technology, Hebei North University, Zhangjiakou 075000, China
Chunchao Zhao
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
Mengxia Wei
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
Junling Liu
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
Yuangang Zu
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
Fei Wang
Landscape Architecture College, Northeast Forestry University, Harbin 150040, China
Lei Yang
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China

Abstract


In order to hydrolyze geniposide and extract geniposidic acid, a novel microwave irradiation heating method for simultaneous hydrolysis and extraction for preparation of geniposidic acid from Eucommia ulmoides bark using basic imidazolide ionic liquid was developed in this work. Basic ionic liquid paired by imidazolide anion and 1-hexyl-3-methylimidazolium cation ([C6mim]Im) was used as a dual hydrolysis catalyst and extraction solvent in the proposed approach.The optimal concentration of [C6mim]Im was 0.4 mol/L. And the independent parameters optimized by Box-Behnken design (BBD) based on single-factor tests were as follows: microwave irradiation time of 20 min at power of 415Wand liquid-solid ratio of 15 mL/g. Yield of geniposidic acid was increased to 3.41±0.17 μmol/g. DM130macroporous resin, after reaction, was selected to separate geniposidic acid fromhydrolysate.The plant materials with different treatment were analyzed by scanning electron microscopy (SEM) and thermogravimetric analysis (TG). The analysis of SEM and TG further indicated that IMSHE is an efficient preparation method for geniposidic acid. It is reasonable to assume that the method and the dual hydrolysis catalyst and extraction solvent would offer an alternative for the preparation of some other kinds of bioactive substances from plant materials.