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Das, B. C.
- Studies on the Growth and Multiplication of Trichoderma harzianum and Trichoderma viride on Different Compost Manures and their Population Dynamics in Vitro and in Vivo Conditions
Abstract Views :210 |
PDF Views:123
Authors
Affiliations
1 Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam, IN
1 Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam, IN
Source
Journal of Biological Control, Vol 24, No 3 (2010), Pagination: 253-256Abstract
Compost, biofertilizers and antagonists have been widely explored as effective and ecofriendly options for controlling plant diseases. An investigation was conducted to study in vitro growth and multiplication of talc-based Trichoderma harzianum and a commercial formulation of Trichoderma viride in different compost manures. Optimal compost and vermicompost supported excellent growth of both T. harzianum and T. viride, however, the efficacy of the mixed formulation of both optimal compost and vermicompost resulted in higher population of both the antagonists and they showed significant increase for up to 45 days after inoculation. Shelf life of both the antagonists was studied in vitro and in vivo over a period of 120 days. Higher population (cfu g−1) was reported in optimal compost+vermicompost+T. harzianum, which was significantly higher than optimal compost+vermicompost +T. viride. The maximum population in vitro was recorded at 30 days of incubation, whereas it was recorded in 60 days of incubation in vivo.Keywords
Biological Control, Management, Compost, Trichoderma harzianum, Trichoderma viride.- Eco-Friendly Strategies for Management of Sclerotinia Rot of French Bean
Abstract Views :199 |
PDF Views:107
Authors
Affiliations
1 Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, Assam, IN
2 Department of Life Sciences, Dibrugarh University, Dibrugarh, 786001, Assam, IN
1 Department of Plant Pathology, Assam Agricultural University, Jorhat, 785013, Assam, IN
2 Department of Life Sciences, Dibrugarh University, Dibrugarh, 786001, Assam, IN
Source
Journal of Biological Control, Vol 22, No 2 (2008), Pagination: 405-410Abstract
Sclerotinia rot caused by Sclerotinia sclerotiorum is a serious menace to the cultivation of french bean (Phaseolus vulgaris L.) in Assam. In vitro efficacy of antagonist by dual culture method was tested against S. sclerotiorum. Out of all the antagonists Pseudomonas fluorescens was found best causing growth reduction of 64.93 per cent followed by Bacillus snbtilis (62.86 %) and Trichoderma harzianum (59.08 %). Study on the mode of parasitism showed that the fungal antagonist initially caused coining and then lysed the pathogen hyphae and the bacterial antagonist ceased pathogen growth after coming in contact with the pathogen. Effect of fungal antagonist T. harzianum and bacterial antagonist's B. subtilis and P. fluorescens as seed treatment against Sclerotinia rot was evaluated under field conditions. Seed treatment with T. harzianum was found has most effective in improving seed germination (18.43%), reducing 90.46% infection and increasing yield (69.51%) over control plot. Efficacy of B. subtilis and P. fluorescence as seed treatment against the disease was next to T. harzianum treatment respectively.Keywords
Antagonist, Biological Control, French Bean, Seed Treatment, Sclerotinia Rot.- Bioformulation of Trichoderma harzianum Rifai for Management of Soybean Stem-Rot Caused by Rhizoctonia solani Kuhn
Abstract Views :279 |
PDF Views:147
Authors
Affiliations
1 Department of Plant Pathology, Assam Agricultural University, Jorhat, 785 013, Assam, IN
1 Department of Plant Pathology, Assam Agricultural University, Jorhat, 785 013, Assam, IN
Source
Journal of Biological Control, Vol 20, No 1 (2006), Pagination: 57-64Abstract
In vitro osmoticant (manitol) amended and osmoticant free media of potato dextrose broth (PDB), modified Richard's broth (MRB) and Czapek dox broth (CDB) were tested for biomass production of Trichoderma harzianum Rifai. Osmoticant amended MRB was found best for production of maximum sporulation, cfu and dry weight of biomass of the antagonist, Osmoticant amended media produced higher biomass of T. harzianum than osmoticant free media. Growth and sporulation of T. harzianum was assessed in different carrier viz. starch, talc and molasses enriched charcoal powder (MECP) based formulation. Starch and MECP based formulation of T. harzianum showed increased trend in sporulation of the antagonist upto 60 days, but after this, viability of T. harzianum was abruptly decreased. Talc based formulation-exhibited gradual declining trend in multiplication and sporulation of T. harzianum after 30 days onwards. Among the nine formulation evaluated for growth and sporulation, starch based formulation alone performed best upto 60 days, followed by MECP based formulation. But after 60 days onwards, talc based formulation @ 3: 1 dose showed highest sporulation and maximum cfu, followed by starch formulation @ 1: 1 dose. The overall performance of talc-based formulation was better for growth and sporulation of the antagonist. Potentiality of T. harzianum as seed treatment with talc based T. harzianum and chemical were assessed field condition against Rhizoctonia solani causing stem rot disease of soybean. Lowest disease index and highest % seed germination was observed when seeds were treated with formulation of T. harzianum + talc + osmoticant formulation with enhanced plant vigour and yield of the crop.Keywords
Biomass, Osmoticant, Rhizoctonia solani, Soybean, Stem Rot, Trichoderma harzianum.- Induction of UV - Radiated Mutant of Trichorderma harzianum Rifai and its Antagonistic Effect on Sclerotinia sclerotiorum (Lib) de Bary in Vitro
Abstract Views :213 |
PDF Views:125
Authors
Affiliations
1 Assam Agricultural University, Jorhat 785013, Assam, IN
1 Assam Agricultural University, Jorhat 785013, Assam, IN
Source
Journal of Biological Control, Vol 20, No 2 (2006), Pagination: 205-211Abstract
In the present investigation, carbendazim tolerance in Trichorderma harzianum was induced by exposing the culture to UV radiation for different periods (viz. 0, 2, 4, 6 and 8 h) and then the same were grown in PDA amended with different concentration of carbendazim viz. 0.01, 0.05, 0.1 and 1.0 per cent, respectively. In each concentration, colonies developed after 5 days were isolated, purified with serial dilution and sub-cultured thrice on PDA without carbendazim and tested their stability. These resulted in the development of 18 stable mutants, which could tolerate up to 0,1 per cent of carbendazim while wild T. harzianum (Th-w) failed to tolerate the same. Based on radial growth of above 18 stable mutants grown on fungicide free PDA, only three stable mutants were selected, which were designed as Th-M1, Th-M2 and Th-M3, In vitro antagonistic activities of these three mutants tested against Sclerotinia sclerotiorum, the causal agent of white mold of French bean on PDA showed maximum reduction of radial growth of pathogen by Th-M2 In another test, all the mutants arrested sclerotial production of S. sclerotiorum, significantly. However, the mutant Th-M2 showed maximum reduction when it was inoculated seven days prior to inoculation with S. sclerotiorum in MSM (4%) as compared to wild T. harzianum (Th-w).Keywords
Carbendazim, Mutant, Sclerotinia sclerotiorum, T. harzianum, UV Radiation, White Mold.- Integrated Management of Soybean Stem Rot
Abstract Views :218 |
PDF Views:115
Authors
Affiliations
1 Department of Plant Pathology, Assam Agricultural University Jorhat 785 013, Assam, IN
1 Department of Plant Pathology, Assam Agricultural University Jorhat 785 013, Assam, IN
Source
Journal of Biological Control, Vol 14, No 1 (2000), Pagination: 67-69Abstract
Combination of four different carriers along with Trichoderma harzianum as seed treatement was tested in pot condition against Rhizoctonia solani causing soybean stem rot. Conidial suspension (1 x 108 conidia per ml) of the antagonist was used for seed treatment. Seed germination (%) and yield (g/plant) was found higher in all treatments irrespective of carrier and antagonist used. However, seed germination and yield was found to he on par. The higbest germination (85.33%) and yield (11.78 g/plant) and lowest disease index (1.5) were obtained when seeds were treated with T. harzianum + methylcellulose. Seed treatment with sub-lethal doses of thiram (0.05 and 0.01 %) and T. harzianum showed lower disease index, higher germination and yield than the lethal dose (0.3 %) alone.Keywords
Carrier, Disease Index, Methylcellulose, Rhizoctonia solani, Thiram, Trichoderma harzianum.- Morphological and Pathogenic Variability in French Bean Isolates of Sclerotinia sclerotiorum
Abstract Views :238 |
PDF Views:0
Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, Assam Agricultural University, Jorhat (Assam), IN
2 Regional Agricultural Research Station, Titabor, Jorhat (Assam), IN
1 Department of Plant Pathology, College of Agriculture, Assam Agricultural University, Jorhat (Assam), IN
2 Regional Agricultural Research Station, Titabor, Jorhat (Assam), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 340-344Abstract
Studies conducted on morphological and pathogenic variability among 30 isolates of Sclerotinia sclerotiorum (Lib) de Bary, derived from infected French bean (Phaseolus vulgaris) plants showing typical symptoms of white mold, collected from different geographical locations of NE India. All the isolates showed variation in morphological characters based on their mycelial growth, colony character and sclerotial formations when grown on PDA. Out of 30 isolates, 14 isolates showed highly virulent reaction, each 8 isolate revealed moderately and less virulent reaction respectively on French bean under pot condition.Keywords
Sclerotinia sclerotiorum, Phaseolus vulgaris, White Mold, PDA, Virulent.References
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- Bag, T.K. (2000). An outbreak of watery pod rot of French bean in the hills of Arunachal Pradesh. J. Mycol. Plant Pathol., 30 (1): 130.
- Cessna, S.G., Sears, V.E., Dickman, M.B. and Low, P.S. (2000). Oxalic acid, a pathogenicity factor for Sclerotinia sclerotiorum, suppresses the oxidative burst of the host plant. Plant Cell, 12 : 2191-2199.
- Dhawan, S., Rai, J.N. and Srivastava, S.K. (1981).Variations in the sulphur content of Brassica juncea plants infected with different isolates of S. sclerotiorum. Indian J. Mycol. PI. Pathol., 11: 100-101.
- Dutta, P. (2006). Biological management of white mold/rot of French bean caused by Sclerotinia sclerotiorum (Lib.) de Bary By some local antagonists, Ph.D (Ag.). Thesis, Assam Agricultural University, Jorhat, ASSAM (INDIA).
- Garg, H., Kohn, Linda M., Andrew, M., Li, H., Krishnapillai S. and Barbetti, M. J.(2010). Pathogenicity of morphologically different isolates of Sclerotinia sclerotiorum with Brassica napus and B. juncea genotypes. European J. Plant Patholol., 126: 305-315.
- Godoy, G., Steadman, J.R., Dickman, M.B. and Dam, R. (1990). Use of mutants to demonstrate the role of oxalic acid in pathogenicity of Sclerotinia sclerotiorum on Phaseolus vulgaris. Physiol. Mol. Plant Pathol, 37: 179–191.
- Iqbal, S.M., Ghafoor, A., Ahmad, Z. and Haqqani, A.M. (2005). Pathogenicity and fungicidal efficacy for Sclerotinia rot of brinjal. Intern. J. Agric. Biol, 5 (4): 618–620.
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- Rai, J.N. and Dhawan, S. (1976). Studies on purification and identification of toxic metabolites produced by Sclerotinia sclerotiorum causing white rot disease of crucifers.Indian Phytopath., 29: 407.
- Saharan, G.S. and Mehta, N. (2008).Sclerotinia diseases of crop plants: Biology, Ecology and Disease Management, Springer Press.
- Steadman, J.R. (1983).White mold- a serious yield limiting disease of bean. Plant Dis., 67 : 346-350.
- Tores, J.A. (1990). In vitro variability of a number of characteristics of S.sclerotiorum. Phytoparasitica, 18 : 321-329.
- Tu, J.C. (1989). Management of white mold of white beans in Ontario. Plant Dis., 73(4): 281-285.
- Willets, H.J. and Wong, J.A.L. (1980). The biology of S. sclerotiorum, S. trifoliorum and S. minor with emphasis on specific nomenclature.Botanical Rev., 46 : 101-165.
- Yu, X., Li, B., Fu, Y., Jiang, D., Ghabrial, S. A., Li, G., Peng, Y., Xie, J., Cheng, J., Huang, J. and Yi, X. (2010). A Gemini virus - related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proceedings of the National Academy of Sciences of the United States of America, 107(18): 8387–8392.
- Big Science and Technology News and Outstanding Scientists and Engineers in the 20th Century
Abstract Views :112 |
PDF Views:0
Authors
Affiliations
1 TTTl, Calcutta, IN
1 TTTl, Calcutta, IN
Source
Indian Science Cruiser, Vol 9, No 1 (1995), Pagination: 7-9Abstract
No Abstract.- Social History of Science Education — Some Remarks Based on P C Bhattacharyya's Recollections
Abstract Views :87 |
PDF Views:0
Authors
Affiliations
1 Calcutta, IN
1 Calcutta, IN
Source
Indian Science Cruiser, Vol 12, No 3 (1998), Pagination: 17-17Abstract
No Abstract.Keywords
No Keywords.- World Education Report 1998 Chief Editor : John Smith [teachers and Teaching in a Changing World] UNESCO Publishing 1998
Abstract Views :73 |
PDF Views:0
Authors
Source
Indian Science Cruiser, Vol 14, No 2 (2000), Pagination: 40-41Abstract
No abstract.Keywords
No keywords.References
- No references.
- World Science Report 1998. UNESCO Publishing Elsevier
Abstract Views :79 |
PDF Views:0
Authors
Source
Indian Science Cruiser, Vol 14, No 3 (2000), Pagination: 43-44Abstract
No abstract.Keywords
No keywords.References
- No references.