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High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis


Affiliations
1 Heart Centre and Department of Public Health and Clinical Medicine, Division of Medicine, Umea University, 901 85 Umea, Sweden
2 Department of Cardiology, Oslo University Hospital, K.G. Jebsen Cardiac Research Center and Center for Heart Failure Research, University of Oslo, 0327 Oslo, Norway
3 Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, 751 23 Uppsala, Sweden
4 Division of Integrative Physiology, Department of Medical Cell Biology, Uppsala University, 751 23 Uppsala, Sweden
5 Department of Medical Sciences, Uppsala University, 751 85 Uppsala, Sweden
6 Division of Medicine, Department of Public Health and Clinical Medicine, Umea University, 901 85 Umea, Sweden
7 Department of Medical Biochemistry and Biophysics-Unit of Research Education and Development Ostersund, Umea University, 831 31 Umea, Sweden
 

Hyaluronan is a negatively charged polydisperse polysaccharide where both its size and tissue concentration play an important role in many physiological and pathological processes. The various functions of hyaluronan depend on its molecular size. Up to now, it has been difficult to study the role of hyaluronan in diseases with pathological changes in the extracellular matrix where availability is low or tissue samples are small. Difficulty to obtain large enough biopsies from human diseased tissue or tissue from animal models has also restricted the study of hyaluronan. In this paper, we demonstrate that gas-phase electrophoretic molecular mobility analyzer (GEMMA) can be used to estimate the distribution of hyaluronan molecular sizes in biological samples with a limited amount of hyaluronan. The low detection level of the GEMMA method allows for estimation of hyaluronanmolecular sizes from different parts of small organs. Hence, the GEMMA method opens opportunity to attain a profile over the distribution of hyaluronan molecular sizes and estimate changes caused by disease or experimental conditions that has not been possible to obtain before.
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  • High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis

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Authors

Lan Do
Heart Centre and Department of Public Health and Clinical Medicine, Division of Medicine, Umea University, 901 85 Umea, Sweden
Christen P. Dahl
Department of Cardiology, Oslo University Hospital, K.G. Jebsen Cardiac Research Center and Center for Heart Failure Research, University of Oslo, 0327 Oslo, Norway
Susanne Kerje
Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, 751 23 Uppsala, Sweden
Peter Hansell
Division of Integrative Physiology, Department of Medical Cell Biology, Uppsala University, 751 23 Uppsala, Sweden
Stellan Morner
Heart Centre and Department of Public Health and Clinical Medicine, Division of Medicine, Umea University, 901 85 Umea, Sweden
Ulla Lindqvist
Department of Medical Sciences, Uppsala University, 751 85 Uppsala, Sweden
Engstrom-Laurent Anna
Division of Medicine, Department of Public Health and Clinical Medicine, Umea University, 901 85 Umea, Sweden
Goran Larsson
Department of Medical Biochemistry and Biophysics-Unit of Research Education and Development Ostersund, Umea University, 831 31 Umea, Sweden
Urban Hellman
Heart Centre and Department of Public Health and Clinical Medicine, Division of Medicine, Umea University, 901 85 Umea, Sweden

Abstract


Hyaluronan is a negatively charged polydisperse polysaccharide where both its size and tissue concentration play an important role in many physiological and pathological processes. The various functions of hyaluronan depend on its molecular size. Up to now, it has been difficult to study the role of hyaluronan in diseases with pathological changes in the extracellular matrix where availability is low or tissue samples are small. Difficulty to obtain large enough biopsies from human diseased tissue or tissue from animal models has also restricted the study of hyaluronan. In this paper, we demonstrate that gas-phase electrophoretic molecular mobility analyzer (GEMMA) can be used to estimate the distribution of hyaluronan molecular sizes in biological samples with a limited amount of hyaluronan. The low detection level of the GEMMA method allows for estimation of hyaluronanmolecular sizes from different parts of small organs. Hence, the GEMMA method opens opportunity to attain a profile over the distribution of hyaluronan molecular sizes and estimate changes caused by disease or experimental conditions that has not been possible to obtain before.