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A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips


Affiliations
1 Department of Biology, College of Arts & Sciences, Barry University, Miami Shores, FL, United States
2 Biomedical Sciences Program and Clinical Biology, College of Nursing and Health Sciences, Barry University,Miami Shores, FL, United States
3 Division of Hematopathology and Laboratory Medicine, Department of Oncologic Sciences, University of South Florida and H. Lee Moffitt Cancer Center, Tampa, FL, United States
 

Cell staining is a necessary and useful technique for visualizing cell morphology and structure under a microscope. This technique has been used in many areas such as cytology, hematology, oncology, histology, virology, serology, microbiology, cell biology, and immunochemistry. One of the key pieces of equipment for preparing a slide for cell staining is cytology centrifuge (cytocentrifuge) such as cytospin. However, many small labs do not have this expensive equipment and its accessory, cytoclips (also expensive relatively), which makes them difficult to study cell cytology. Here we present an alternative method for preparing a slide and cell staining in the absence of a cytocentrifuge (and cytoclips). This method is based on the principle that a regular cell centrifuge can be used to concentrate cells harvested fromcell culture and then deposit the concentrated cell suspension to a slide evenly by using a cell spreader, followed by cell staining. The method presented is simple, rapid, economic, and efficient. This method may also avoid a possible change in cell morphology induced by cytocentrifuge.
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  • A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips

Abstract Views: 84  |  PDF Views: 0

Authors

Xiaotang Hu
Department of Biology, College of Arts & Sciences, Barry University, Miami Shores, FL, United States
Verronika Laguerre
Biomedical Sciences Program and Clinical Biology, College of Nursing and Health Sciences, Barry University,Miami Shores, FL, United States
Daniel Packert
Biomedical Sciences Program and Clinical Biology, College of Nursing and Health Sciences, Barry University,Miami Shores, FL, United States
Alice Nakasone
Department of Biology, College of Arts & Sciences, Barry University, Miami Shores, FL, United States
Lynn Moscinski
Division of Hematopathology and Laboratory Medicine, Department of Oncologic Sciences, University of South Florida and H. Lee Moffitt Cancer Center, Tampa, FL, United States

Abstract


Cell staining is a necessary and useful technique for visualizing cell morphology and structure under a microscope. This technique has been used in many areas such as cytology, hematology, oncology, histology, virology, serology, microbiology, cell biology, and immunochemistry. One of the key pieces of equipment for preparing a slide for cell staining is cytology centrifuge (cytocentrifuge) such as cytospin. However, many small labs do not have this expensive equipment and its accessory, cytoclips (also expensive relatively), which makes them difficult to study cell cytology. Here we present an alternative method for preparing a slide and cell staining in the absence of a cytocentrifuge (and cytoclips). This method is based on the principle that a regular cell centrifuge can be used to concentrate cells harvested fromcell culture and then deposit the concentrated cell suspension to a slide evenly by using a cell spreader, followed by cell staining. The method presented is simple, rapid, economic, and efficient. This method may also avoid a possible change in cell morphology induced by cytocentrifuge.