International Journal of Genomics

Bharat Bhusan Patnaik ¹, So Young Park ¹, Se Won Kang ¹, Hee-Ju Hwang ¹, Tae Hun Wang ¹, Eun Bi Park ¹, Jong Min Chung ¹, Dae Kwon Song ¹, Changmu Kim ², Soonok Kim ², Jae Bong Lee ³, Heon Cheon Jeong ⁴, Hong Seog Park ⁵, Yeon Soo Han ⁶, Yong Seok Lee ⁷

Affiliations
1 Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, KR
2 National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, KR
3 Korea Zoonosis Research Institute (KOZRI), Chonbuk National Institute, 820-120 Hana-ro, Iksan, Jeollabuk-do 54528, KR
4 Hampyeong County Insect Institute, Hampyeong County Agricultural Technology Center, 90 Hakgyohwasan-gil, Hakgyo-myeon, Jeollanam-do 57158, KR
5 Research Institute, GnC BIO Co., Ltd., 621-6 Banseok-dong, Yuseong-gu, Daejeon 34069, KR
6 College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, KR
7 Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, KR

Abstract

Vespa mandarinia found in the forests of East Asia, including Korea, occupies the highest rank in the arthropod food web within its geographical range. It serves as a source of nutrition in the form of Vespa amino acid mixture and is listed as a threatened species, although no conservation measures have been implemented. Here, we performed de novo assembly of the V. mandarinia transcriptome by Illumina HiSeq 4000 sequencing. Over 60 million raw reads and 59,184,811 clean reads were obtained. After assembly, a total of 66,837 unigenes were clustered, 40,887, 44,455, and 22,390 of which showed homologous matches against the PANM, Unigene, and KOG databases, respectively. A total of 15,675 unigenes were assigned to Gene Ontology terms, and 5,132 unigeneswere mapped to 115 KEGG pathways.The zinc finger domain (C2H2-like), serine/threonine/dual specificity protein kinase domain, and RNA recognition motif domain were among the top InterProScan domains predicted for V. mandarinia sequences. Among the unigenes, we identified 534,922 cDNA simple sequence repeats as potential markers. This is the first transcriptomic analysis of the wasp V. mandarinia using Illumina HiSeq 4000.The obtained datasets should promote the search for new genes to understand the physiological attributes of this wasp.

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