Open Access Open Access  Restricted Access Subscription Access

Transcriptome Profile of the Asian Giant Hornet (Vespa mandarinia) Using Illumina HiSeq 4000 Sequencing: De Novo Assembly, Functional Annotation, and Discovery of SSR Markers


Affiliations
1 Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
2 National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea, Republic of
3 Korea Zoonosis Research Institute (KOZRI), Chonbuk National Institute, 820-120 Hana-ro, Iksan, Jeollabuk-do 54528, Korea, Republic of
4 Hampyeong County Insect Institute, Hampyeong County Agricultural Technology Center, 90 Hakgyohwasan-gil, Hakgyo-myeon, Jeollanam-do 57158, Korea, Republic of
5 Research Institute, GnC BIO Co., Ltd., 621-6 Banseok-dong, Yuseong-gu, Daejeon 34069, Korea, Republic of
6 College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea, Republic of
7 Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
 

Vespa mandarinia found in the forests of East Asia, including Korea, occupies the highest rank in the arthropod food web within its geographical range. It serves as a source of nutrition in the form of Vespa amino acid mixture and is listed as a threatened species, although no conservation measures have been implemented. Here, we performed de novo assembly of the V. mandarinia transcriptome by Illumina HiSeq 4000 sequencing. Over 60 million raw reads and 59,184,811 clean reads were obtained. After assembly, a total of 66,837 unigenes were clustered, 40,887, 44,455, and 22,390 of which showed homologous matches against the PANM, Unigene, and KOG databases, respectively. A total of 15,675 unigenes were assigned to Gene Ontology terms, and 5,132 unigeneswere mapped to 115 KEGG pathways.The zinc finger domain (C2H2-like), serine/threonine/dual specificity protein kinase domain, and RNA recognition motif domain were among the top InterProScan domains predicted for V. mandarinia sequences. Among the unigenes, we identified 534,922 cDNA simple sequence repeats as potential markers. This is the first transcriptomic analysis of the wasp V. mandarinia using Illumina HiSeq 4000.The obtained datasets should promote the search for new genes to understand the physiological attributes of this wasp.
User
Notifications
Font Size

Abstract Views: 50

PDF Views: 0




  • Transcriptome Profile of the Asian Giant Hornet (Vespa mandarinia) Using Illumina HiSeq 4000 Sequencing: De Novo Assembly, Functional Annotation, and Discovery of SSR Markers

Abstract Views: 50  |  PDF Views: 0

Authors

Bharat Bhusan Patnaik
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
So Young Park
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
Se Won Kang
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
Hee-Ju Hwang
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
Tae Hun Wang
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
Eun Bi Park
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
Jong Min Chung
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
Dae Kwon Song
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of
Changmu Kim
National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea, Republic of
Soonok Kim
National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon 22689, Korea, Republic of
Jae Bong Lee
Korea Zoonosis Research Institute (KOZRI), Chonbuk National Institute, 820-120 Hana-ro, Iksan, Jeollabuk-do 54528, Korea, Republic of
Heon Cheon Jeong
Hampyeong County Insect Institute, Hampyeong County Agricultural Technology Center, 90 Hakgyohwasan-gil, Hakgyo-myeon, Jeollanam-do 57158, Korea, Republic of
Hong Seog Park
Research Institute, GnC BIO Co., Ltd., 621-6 Banseok-dong, Yuseong-gu, Daejeon 34069, Korea, Republic of
Yeon Soo Han
College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea, Republic of
Yong Seok Lee
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, 22 Soonchunhyangro, Shinchang-myeon, Asan, Chungchungnam-do 31538, Korea, Republic of

Abstract


Vespa mandarinia found in the forests of East Asia, including Korea, occupies the highest rank in the arthropod food web within its geographical range. It serves as a source of nutrition in the form of Vespa amino acid mixture and is listed as a threatened species, although no conservation measures have been implemented. Here, we performed de novo assembly of the V. mandarinia transcriptome by Illumina HiSeq 4000 sequencing. Over 60 million raw reads and 59,184,811 clean reads were obtained. After assembly, a total of 66,837 unigenes were clustered, 40,887, 44,455, and 22,390 of which showed homologous matches against the PANM, Unigene, and KOG databases, respectively. A total of 15,675 unigenes were assigned to Gene Ontology terms, and 5,132 unigeneswere mapped to 115 KEGG pathways.The zinc finger domain (C2H2-like), serine/threonine/dual specificity protein kinase domain, and RNA recognition motif domain were among the top InterProScan domains predicted for V. mandarinia sequences. Among the unigenes, we identified 534,922 cDNA simple sequence repeats as potential markers. This is the first transcriptomic analysis of the wasp V. mandarinia using Illumina HiSeq 4000.The obtained datasets should promote the search for new genes to understand the physiological attributes of this wasp.