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Kar, Sanjay
- Enhanced Production of α-Amylase from Bacillus licheniformis SKB 4
Abstract Views :391 |
PDF Views:3
Authors
Saptadip Samanta
1,
Sanjay Kar
2,
Pradeep Kumar Das Mohapatra
3,
Bikash Ranjan Pati
3,
Keshab Chandra Mondal
3
Affiliations
1 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
2 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
3 Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, IN
1 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
2 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
3 Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, IN
Source
Indian Science Cruiser, Vol 31, No 5 (2017), Pagination: 32-39Abstract
The effect of some inductive factors including incubation temperature, aeration, inorganic phosphate source, supplementation of amino acids and vitamins on production of extracellular amylase by Bacillus licheniformis SKB 4 was optimized in a submerged fermentation. The maximum production of enzyme was obtained in highly aerobic condition at 42°C, pH 6.5 and 24 h incubation period. 1%, v/v inoculumn concentration exerts best result for growth and enzyme production. Several chemical factors such as inorganic phosphate (KH2PO4; 0.3%), amino acids (0.5 mg %; glutamic acid, cysteine, isoleucine), vitamins (biotin, 50μg/ml; pyridoxine, 50μg/ml; riboflavin, 20μg/ml), metallic salt MgSO4 (1 mM) and surfactant Tween 80 (0.075% (v/v) have positive effect on amylase production. Among the raw food grains used for amylase production, soya bean seed favoured enzyme production and rate production is much higher (2.5 fold) than the normal culture medium, whereas cereals and other pulses have no effect on enzyme production within normal incubation time. Use of raw food grain as culture medium and optimization of certain factors are the most important part for production of amylase in industrial sector.Keywords
Inductive Factors of Amylase Production from Bacillus licheniformis SKB4.
References
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- Studies on Regulation of Xylanase Bio-Synthesis by Trichoderma reesei SAF3
Abstract Views :337 |
PDF Views:3
Authors
Affiliations
1 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
2 PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, IN
3 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
4 Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, IN
1 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
2 PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, IN
3 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
4 Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, IN
Source
Indian Science Cruiser, Vol 33, No 3 (2019), Pagination: 19-26Abstract
Microbial xylanases have an exciting potentiality and are being used extensively in different industries. In this study, regulation of xylanase bio-synthesis was examined in Trichoderma reesei SAF3. Xylan (1.0% w/v) showed the highest inductive effect. Glucose at concentration of 1% (w/v) was repressive for xylanase bio-synthesis when used in presence or absences of xylan; the repression was partially elevated by addition of cyclic adenosine monophosphate (cAMP, 5mM). The above findings suggested that xylan was the major inducer and the induction was energy dependent, mediated by second messenger system.Keywords
Xylanase, Regulation of Biosynthesis, cAMP, Trichoderma Reesei.References
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