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Biochemical Study of Root Extract of Withania somnifera (L.) Plant through HPLC Analysis


Affiliations
1 Department of Biotechnology, S.G.R.R.P.G College, Dehradun, United Kingdom
2 Department of Biotechnology, S.G.R.R.P.G College, Dehradun, India
3 Department of Microbiology, S.G.R.R.P.G College, Dehradun, United Kingdom
4 Department of Environmental Biology, A.P.S. University, Rewa, M.P., India
5 Department of Botany, Govt. P.G. Science College, Rewa, M.P., India
     

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Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and assessment of their capability in production and accumulation of bioactive metabolites (total alkaloid and with anolides amount were quantified; withanolide A and withaferin A contents were estimated by High Performance Liquid Chromatography-HPLC).


Keywords

Bioactive Chemicals, Culture Condition, Ex vitro, In vitro, Mass Production, Micro-Shoots, Secondary Metabolite, W. somnifera.
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  • Biochemical Study of Root Extract of Withania somnifera (L.) Plant through HPLC Analysis

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Authors

Naveen Gaurav
Department of Biotechnology, S.G.R.R.P.G College, Dehradun, United Kingdom
Arun Kumar
Department of Biotechnology, S.G.R.R.P.G College, Dehradun, United Kingdom
Aditi Grover
Department of Biotechnology, S.G.R.R.P.G College, Dehradun, India
Deepak Som
Department of Microbiology, S.G.R.R.P.G College, Dehradun, United Kingdom
U. K. Chauhan
Department of Environmental Biology, A.P.S. University, Rewa, M.P., India
A. P. Singh
Department of Botany, Govt. P.G. Science College, Rewa, M.P., India

Abstract


Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and assessment of their capability in production and accumulation of bioactive metabolites (total alkaloid and with anolides amount were quantified; withanolide A and withaferin A contents were estimated by High Performance Liquid Chromatography-HPLC).


Keywords


Bioactive Chemicals, Culture Condition, Ex vitro, In vitro, Mass Production, Micro-Shoots, Secondary Metabolite, W. somnifera.