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Cytomorphometric Characteristics of Buccal Mucosal Cells in Behçet’s Disease Patients


Affiliations
1 School of Medicine, Department of Family Medicine, Bulent Ecevit University, 67600 Zonguldak, Turkey
2 School of Medicine, Department of Medical Biology, Bulent Ecevit University, 67600 Zonguldak, Turkey
3 Department of Pathology, Taksim Education and Research Hospital, 34433 Istanbul, Turkey
4 School of Medicine, Department of Dermatology, Selcuk University, 42075 Konya, Turkey
5 School of Medicine, Department of Dermatology, Bulent Ecevit University, 67600 Zonguldak, Turkey
 

Background. The aim of this study was to compare the cytomorphometric characteristics of the buccal cells of Behc¸et's disease patients with those of healthy controls. Methods. This case-control study compared a group of 30 patients with Behc¸et's disease with an age- and gender-matched control group of 30 healthy individuals. The buccal mucosal smears were stained using the Papanicolaou technique for cytomorphometric analyses. The nuclear and cytoplasmic areas were evaluated using digital image analysis; the ratio of nuclear to cytoplasmic areas and nuclear roundness are presented. Results.The nuclear and cytoplasmic areas of the BD patients' cells were significantly smaller than those of the healthy controls' cells, while the nucleus-to-cytoplasm ratio and neutrophil infiltration rate did not differ significantly between the groups. However, the nuclear area, cytoplasmic area, nucleusto- cytoplasm ratio, and nuclear roundness factor were significantly higher in patients without aphthae. The neutrophil infiltration rate did not differ significantly in patients with or without aphthae. Conclusion. Behc¸et's disease can produce cytomorphometric changes in buccal cells that are detectable by exfoliative cytology and cytomorphometric analysis techniques.
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  • Cytomorphometric Characteristics of Buccal Mucosal Cells in Behçet’s Disease Patients

Abstract Views: 141  |  PDF Views: 26

Authors

Erol Aktunc
School of Medicine, Department of Family Medicine, Bulent Ecevit University, 67600 Zonguldak, Turkey
Zehra Safi Oz
School of Medicine, Department of Medical Biology, Bulent Ecevit University, 67600 Zonguldak, Turkey
Sibel Bektas
Department of Pathology, Taksim Education and Research Hospital, 34433 Istanbul, Turkey
Cevdet Altinyazar
School of Medicine, Department of Dermatology, Selcuk University, 42075 Konya, Turkey
Rafet Koca
School of Medicine, Department of Dermatology, Bulent Ecevit University, 67600 Zonguldak, Turkey
Serdar Bostan
School of Medicine, Department of Dermatology, Bulent Ecevit University, 67600 Zonguldak, Turkey

Abstract


Background. The aim of this study was to compare the cytomorphometric characteristics of the buccal cells of Behc¸et's disease patients with those of healthy controls. Methods. This case-control study compared a group of 30 patients with Behc¸et's disease with an age- and gender-matched control group of 30 healthy individuals. The buccal mucosal smears were stained using the Papanicolaou technique for cytomorphometric analyses. The nuclear and cytoplasmic areas were evaluated using digital image analysis; the ratio of nuclear to cytoplasmic areas and nuclear roundness are presented. Results.The nuclear and cytoplasmic areas of the BD patients' cells were significantly smaller than those of the healthy controls' cells, while the nucleus-to-cytoplasm ratio and neutrophil infiltration rate did not differ significantly between the groups. However, the nuclear area, cytoplasmic area, nucleusto- cytoplasm ratio, and nuclear roundness factor were significantly higher in patients without aphthae. The neutrophil infiltration rate did not differ significantly in patients with or without aphthae. Conclusion. Behc¸et's disease can produce cytomorphometric changes in buccal cells that are detectable by exfoliative cytology and cytomorphometric analysis techniques.