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John, Suchit A.
- In Vitro Screening of Plant Growth Promoting Rhizobacteria to Control Bacterial Wilt (Ralstonia solanacearum) of Tomato (Lycopersicon esculentum)
Authors
1 Department of Biological Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 224-227Abstract
Ralstonia solanacearum is the causative agent of bacterial wilt that causes considerable damages in the yield of various crop plants. The intent of the study was to evaluate potential of bacterial antagonists to suppress bacterial wilt disease development and evaluate the role of the strains as plant growth-promoting rhizobacteria (PGPR) in tomato. One hundred rhizosphere bacterial isolates were screened against virulent strain of Ralstonia solanacearum. After in vitro screening 10 antagonistic strains designated PR3, PR17, PR26, 1NAB15, 1NAB20, 3NAA1, 3NAA2, 2CBA2, 2CBA4, 2CBA18 showed antagonistic effect by producing inhibition zone supressing the growth of R. Solanacearum. The isolate PR3 showed the highest inhibition zone measuring 33.3mm whereas 1NAB15 showed the lowest zone measuring 10mm. The present study, therefore, suggests that the use of PGPR isolates which showed the antagonistic activity can be used as inoculants/ bioantagonists might be beneficial for the control of bacterial wilt of tomato in field studies.Keywords
Ralstonia solanacearum, PGPR, Bacterial Wilt, Tomato.References
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- Evaluation of Exogenous Application of 24-Epibrassinolide and Silicon on Mophological Characters of Salt Stressed Wheat Varieties
Authors
1 Department of Biological Sciences, School of Basic Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
3 Department of Biological Sciences, School of Basic Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Science, Vol 11, No 1 (2016), Pagination: 1-5Abstract
Wheat is a major cereal crop in many parts of the world and it is commonly known as king of cereals. Brassinosteroids (BRs) are growth-promoting natural products found at low levels in pollen, seeds, and young vegetative tissues throughout the plant kingdom. An experiment was conducted to evaluate the effect of different combinations of Silicon (Si) and 24-epibrassinolide (EBL) on wheat varieties grown under 100 mM salt stress. The result showed that shoot length of plant increased with the application of EBL and Silicon and it was increased the maximum in salt tolerant varieties in comparison to non-tolerant. Whilst, reduction was observed in ischolar_main length along with the increasing concentration of EBL. The effect of different combinations of silicon and 24-epibrassinolidealso increased the germination percentage (%) in tolerant and non-tolerant wheat varieties.
Keywords
24-Epibrassinolide, Salt Stress, Silicon, Seed Germination, Shoot-Root Length.- Physiological, biochemical and molecular manifestations in response to seed priming with elicitors under drought in cotton
Authors
1 ICAR-Central Institute for Cotton Research, Nagpur 441 108, India; Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 221 007, India
2 ICAR-Central Institute for Cotton Research, Nagpur 441 108, India
3 Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 221 007, India
Source
Current Science, Vol 123, No 5 (2022), Pagination: 658-666Abstract
Water stress has a detrimental effect on growth and development, which alters physio-biochemical activities. Seed priming with elicitors such as methyl jasmonate and paclobutrazol can mitigate the impact of drought stress. Therefore, pot-culture studies were conducted with drought-tolerant (DTS-155) and drought-susceptible (IC-357055) cotton genotypes to assess the seed priming effects of elicitors (methyl jasmonate and paclobutrazol) on the physio-biochemical changes and gene expression. The dose (50, 100, 150 and 200 mM) and time interval (1.5 and 2.5 h) experiments of both the elicitors were performed separately. On the basis of germination, seedling growth and vigour, a 150 mM elicitor for 1.5 h time interval was found to be the best. Biochemical and physiological parameters confirmed an increase in relative water content, total antioxidant activities, chlorophyll, superoxide dismutase, catalase and proline under drought stress in both the genotypes, but a decrease in lipid peroxidation. Among the elicitors, methyl jasmonate improved drought tolerance as compared to paclobutrazol. Gene expression studies with Rub-S, Rub-L and Osmotin confirmed the results. Transcript abundance of Osmotin and Rub-L was upregulated under drought stress in both the genotypes and was highest in methyl jasmonate primed samples. These findings suggest that priming with methyl jasmonate enhances drought tolerance in cottonKeywords
Drought responsive gene, Gossypium hirsu-tum, methyl jasmonate, paclobutrazol, seed primingReferences
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