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Misra, Pragati
- Multiple Sequence Alignments Analysisof Phaseolin Gene Fromphaseolus Vulgaris (common Bean)
Authors
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), IN
2 College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, MEERUT (U.P.), IN
3 Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), IN
Source
Agriculture Update, Vol 9, No 3 (2014), Pagination: 347-353Abstract
Abstract not Given,Keywords
Phaseolus Vulgaris, Phaseolin Gene, Blast Analysis, Multiple Sequence Alignment- Presence of Phytoplasma Infections in Papaya (carica Papaya L.) Plants in Uttar Pradesh, India
Authors
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), IN
Source
International Journal of Plant Protection, Vol 7, No 2 (2014), Pagination: 401-404Abstract
During survey of papaya fields in Meerut, severe symptoms of leaf yellowing, intervenial chlorosis, and curl apical necrosis were observed in approximately 25 per cent of plants in each of the papaya fields. DNA extracted from leaf, midrib and bark of symptomatic and healthy plants of papaya were preceded with universal primer pairs. Expected ~1600 bp fragments were amplified with primer pair P1/Tint in diseased plants. Infection of phytoplasma disease in papaya plants was confirmed by the PCR.Keywords
Papaya, Phytoplasma, Curl Apical Necrosis, Pcr- PCR Based Detection of Phytoplasma Association in Pot Marigold (Calendula officinalis L.) and Guldawari (Dendranthema grandiflora L.)
Authors
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), IN
Source
Asian Journal of Bio Science, Vol 9, No 2 (2014), Pagination: 238-241Abstract
Flowering plants Calendula officinalis with phyllody and virescence and Dendranthema grandiflora with little leaf and formation of bladder like silique symptoms observed in Uttar Pradesh, India. The presence of phytoplasmas in diseased plants was detected by direct and nested polymerase chain reaction assays using phytoplasma-specific primer pairs P1/P7 and R16F2n/R2. In both flowering plants presence of phytoplasma was confirmed by amplification of 1200 bp product of phytoplasma 16S rRNA region with nested primer R16F2n/R2. This is the first report of phytoplasma associated with Calendula officinalis from India.Keywords
Phytoplasma, Virescence, Phyllody, Nested PCRReferences
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- Light-Regulatory Effect on the Phytosynthesis of Silver Nanoparticles Using Aqueous Extract of Garlic (Allium sativum) and Onion (Allium cepa) Bulb
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed to be University), Allahabad 211 007, IN
2 Department of Biological Sciences, School of Basic Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed to be University), Allahabad 211 007, IN
Source
Current Science, Vol 111, No 8 (2016), Pagination: 1364-1368Abstract
Green synthesis emerged as an exciting approach in the field of nanotechnology. Biogenesis of nanosilver is simple, eco-friendly, safe and cost-efficacious and therefore gaining impetus nowadays. An effort has been made to understand the possible induced mechanism for the biosynthesis of silver nanoparticles by exposing a mixture of 1 mM AgNO3 with aqueous extracts of garlic (Allium sativum) and onion (Allium cepa) under light and dark conditions. In the synthesis of silver nanoparticles, components of garlic and onion bulb extract served both as capping and reducing agents, whereas the light acted as catalyst. Green synthesized silver nanoparticles were quantified spectrophotometrically at different time intervals. Scanning electron microscopy confirmed that biosynthesized nanoparticles were polydispersed, spherical in shape and under the size range of 100 nm. Light proved to be an stimulating factor in the green synthesis of silver nanoparticles which may vary from species to species.Keywords
Garlic Bulb, Green Synthesis, Light Effect, Onion Bulb, Silver Nanoparticles.References
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- Evaluation of Exogenous Application of 24-Epibrassinolide and Silicon on Mophological Characters of Salt Stressed Wheat Varieties
Authors
1 Department of Biological Sciences, School of Basic Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
3 Department of Biological Sciences, School of Basic Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Science, Vol 11, No 1 (2016), Pagination: 1-5Abstract
Wheat is a major cereal crop in many parts of the world and it is commonly known as king of cereals. Brassinosteroids (BRs) are growth-promoting natural products found at low levels in pollen, seeds, and young vegetative tissues throughout the plant kingdom. An experiment was conducted to evaluate the effect of different combinations of Silicon (Si) and 24-epibrassinolide (EBL) on wheat varieties grown under 100 mM salt stress. The result showed that shoot length of plant increased with the application of EBL and Silicon and it was increased the maximum in salt tolerant varieties in comparison to non-tolerant. Whilst, reduction was observed in ischolar_main length along with the increasing concentration of EBL. The effect of different combinations of silicon and 24-epibrassinolidealso increased the germination percentage (%) in tolerant and non-tolerant wheat varieties.
Keywords
24-Epibrassinolide, Salt Stress, Silicon, Seed Germination, Shoot-Root Length.- Mass in-vitro Micro Propagation of Banana (Musa sp.)
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
International Journal of Plant Protection, Vol 9, No 1 (2016), Pagination: 204-210Abstract
Production of Banana is constrained by many biotic as well as abiotic factors. The production of disease free planting material of banana by meristem culture technique is an urgent need.An experiment has been designed to develop a suitable and efficient protocol for plant regeneration in banana through micro propagation.The surface sterilization of banana plant is the most difficult practice.So it is very important to optimize the concentration and duration of different sterilants for explants surface sterilization. The best sterilants for the surface sterilization of the explant of banana were 0.1 per cent HgCl2 for 5 min duration followed by NaOCl (40 %, commercially available) solution for 8 min duration and 70 per cent ethyl alcohol for 2 min. And the best medium for establishment of banana explant was supplemented with 3mg/l BAP, 0.2mg/l IAA with 30 mg ascorbic acid followed by Proliferation with 5mg/l BAP, 0.5mg/ l IAA with 30 mg ascorbic acid and 10 mg/l BAP,0.5 mg/l IAA with 30 mg ascorbic acid.Significantly highest number of ischolar_mains and greater ischolar_main length were produced by 0.5 mg/l IAA + 0.5 mg/l IBA.Keywords
In-vitro:In Glass, NaOCl:Sodium Hypochlorite, BAP:6-Benzyl Amino Purine, IAA:Indole-3-Acetic Acid, IBA:Indole-3-Butyric Acid.- Diversity analysis of Lentil (Lens culinaris Medik.) Germplasm using Morphological Markers
Authors
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 College of Biotechnology, Sardar Vallabh Bhai Patel University of Agriculture and Technology, Meerut (U.P.), IN
Source
Asian Journal of Bio Science, Vol 9, No 1 (2014), Pagination: 39-42Abstract
Diversity analysis among the seventy six germplasm of lentil (Lens culinaris Medik.) was estimated using morphological markers. Morphological characterization was done based on different parameters like plant height, primary and secondary branches per plant, number of leaves per plant and fresh weights carried out at different stages of the crop and showed wide variability. Dry matter partitioning viz., leaf dry weight, pods dry weight, total dry weight, yield and yield components, pod per plant, seed per plant, 100 seeds weight and seed yield per plant, were recorded at the time of harvest. The results of present investigation suggested that genetic relationships in lentil germplasm using morphological data may be useful for plant improvement and an efficient way to conserve genetic resources of lentil.