Scientia Pharmaceutica

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Spectrophotometric Determination of Cefepime Hydrochloride, Cefoperazone Sodium, Ceftazidime Pentahydrate, Cefuroxime Sodium and Etamsylate Using Ammonium Molybdate


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1 Analytical Chemistry Department Faculty of Pharmacy - Zagazig University Zagazig, Egypt
     

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A simple, rapid and sensitive spectrophotometric method has been developed for the quantitative determination of five drugs of pharmaceutical interest; cefepime HCl, cefoperazone Na, ceftazidime pentahydrate, cefuroxime Na and etamsylate in pure form as well as in pharmaceuticals. The method is based on the reduction of the chromogenic agent, ammonium molybdate (Mo6+), into molybdenum blue (Mo5+) by the examined drugs in sulphuric acid medium and by aid of heating in boiling water bath. The resulting "blue coloured" product possess a characteristic λmax at 695-71 6 nm. Beers law is obeyed over the concentration range of 2-70 μg ml-1 with molar absorpitivities ranging from 2.704×103-24.14×103 L.mol-1.cm-1 and Sandell sensitivities ranging from 1.03×10-3-5.4×10-3 μg cm-2. The proposed method has been applied successfully for the determination of the examined drugs both in pure form and in pharmaceutical formulations. The accuracy and precision of the proposed method are comparable with those of the reported methods

Keywords

Spectrophotometry, Ammonium Molybdate, Etamsylate Cephalosporins, Dosage Forms.
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  • Spectrophotometric Determination of Cefepime Hydrochloride, Cefoperazone Sodium, Ceftazidime Pentahydrate, Cefuroxime Sodium and Etamsylate Using Ammonium Molybdate

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Authors

Marwa S. Elazazy
Analytical Chemistry Department Faculty of Pharmacy - Zagazig University Zagazig, Egypt
Abdalla Shalaby
Analytical Chemistry Department Faculty of Pharmacy - Zagazig University Zagazig, Egypt
M. N. Elbolkiny
Analytical Chemistry Department Faculty of Pharmacy - Zagazig University Zagazig, Egypt
Hawa M. Khalil
Analytical Chemistry Department Faculty of Pharmacy - Zagazig University Zagazig, Egypt

Abstract


A simple, rapid and sensitive spectrophotometric method has been developed for the quantitative determination of five drugs of pharmaceutical interest; cefepime HCl, cefoperazone Na, ceftazidime pentahydrate, cefuroxime Na and etamsylate in pure form as well as in pharmaceuticals. The method is based on the reduction of the chromogenic agent, ammonium molybdate (Mo6+), into molybdenum blue (Mo5+) by the examined drugs in sulphuric acid medium and by aid of heating in boiling water bath. The resulting "blue coloured" product possess a characteristic λmax at 695-71 6 nm. Beers law is obeyed over the concentration range of 2-70 μg ml-1 with molar absorpitivities ranging from 2.704×103-24.14×103 L.mol-1.cm-1 and Sandell sensitivities ranging from 1.03×10-3-5.4×10-3 μg cm-2. The proposed method has been applied successfully for the determination of the examined drugs both in pure form and in pharmaceutical formulations. The accuracy and precision of the proposed method are comparable with those of the reported methods

Keywords


Spectrophotometry, Ammonium Molybdate, Etamsylate Cephalosporins, Dosage Forms.

References