Research Journal of Pharmacognosy and Phytochemistry

A. Shukla ¹, Papiya Bigoniya ¹

Affiliations
1 Radharaman College of Pharmacy, Ratibad, Bhopal-02, Madhya Pradesh, IN

Abstract

The effect of total alkaloid fraction extracted from the seeds of Lepidium sativum Linn (Cruciferae) on body weight, relative liver weight, biochemical parameters, in vitro bromosulphalein (BSP) uptake and histology of liver in rats intoxicated with carbon tetra chloride (CCl₄) were evaluated in this study. The L. sativum alkaloidal fraction (LSAF) was given in dose of 50, 150 and 250 mg/kg for fourteen days. LSAF showed dose dependent protection against body weight loss. Silymarin and LSAF treatment showed hepatoprotection by reducing the liver weight of CCl₄ intoxicated rats from 4.342 ± 0.035 to 3.986 ± 0.025 gm/100 gm body weight respectively. LSAF at 250 mg/kg dose significantly (p<0.01-0.001) reduced the serum total and direct bilirubin, SGOT, SGPT, ALP, cholesterol and triglyceride compared to CCl₄ treated group. Serum protein and albumin level was extreme significantly (p<0.01-0.001) normalized at 250 mg/kg dose. LSAF at dose of 150 and 250 mg/kg showed 61.22 and 75.02% hepatoprotection in BSP uptake. LSAF treatment following CCl₄ intoxication showed mild focal coagulative and centrolobular necrosis, with altered hepatic parenchyma compared to severe hepatic damage caused by CCl4 in liver section.

Keywords

Lepidium sativum, Imidazole Alkaloid, Hepatoprotective, Bromosulphalein.

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Papiya Bigoniya ¹, C. S. Singh ², B. Shrivastava ³

Affiliations
1 Radharaman College of Pharmacy, Bhadbada Road, Ratibad, Bhopal- 462 002, Madhya Pradesh, IN
2 Radharaman College of Pharmacy, Fatehpur Dobra, Ratibad, Bhopal, Madhya Pradesh, IN
3 School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, IN

Abstract

Alternative and traditional medicines, largely herbal in nature, are now regarded as important but underutilized tools against disease. WHO recognized this fact and encouraged governments to effectively utilize local knowledge of herbal medicines for disease prevention and health promotion. Herbal medicines, however, suffer from a range of shortcomings. These include insufficient and unacceptable evidences of safety, efficacy, standardization and inconsistent production practices. There is a growing concern for documentation of research work carried out on traditional medicines needed for regulatory control. With this backdrop, it becomes extremely important to make an effort towards standardization of plant material used for therapeutic purposes. The process of standardization can be achieved by stepwise pharmacognostical, phyto- and physico-chemical studies and minimizing the inherent variation of natural product composition through quality assurance practices. WHO recommends various physicochemical and phyto-chemical evaluation parameters for standardization and quality control of herbal medicinal plants. In view of ethnopharmacological importance of Cichorium intybus Linn, preliminary phyto-chemical screening and quantitative estimation of seed were performed along with morphology and microscopy to establish the salient diagnostic characters. The characteristics evaluated will be helpful for establishing quantitative and qualitative standardization of herbal preparations containing Cichorium intybus seed.

Keywords

Cichorium intybus Seed, Pharmacognostical, Physicochemical, Phyto-Chemical Studies.

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