Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

LCMS Spectral Analysis of Betacyanin Pigments in Amaranthus L.


Affiliations
1 Depatment of Botany, St.Teresa’s College, Ernakulam, Kerala 682011, India
     

   Subscribe/Renew Journal


Plant pigments has got wider application in textile and pharmaceutical industry. Betacyanins are classes of purple plant pigments that are useful in plant identification and health monitoring. Pure samples of betacyanin were obtained by extracting and isolating the pigments found in Amaranthus L. plants. The resulting samples were then used to identify the absorption spectra of the pigment. The sample were analysed for measuring the absorbance at the range of 476nm and 538nm respectively. Betanin, Neobetanin and Amaranthine were found in the red pigments isolated from the plants which are of pharmaceutically important and got medicinal application. These pigments play important role as antioxidant and a natural dye for different formulations. The findings from the present spectral analysis showed 382 m/z ratio found to be derivative of betanidin component. LCMS analysis of the red pigment were carried out showing positive peak with 389 m/z ratio for betanidin compound among Amaranthus caudatus L. and Amaranthus dubius, Mart along with other peak values. The antioxidant capacity that is to scavenge the free radicals of these two Amaranthus L. species can be taken into account as it is a good dietary supplement in our daily life.

Keywords

Betacyanin, Betanin, Neobetalin , Amaranthine, LCMS.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Francis MA, Peter NU, Patrick I, Julius O O, John R, XiaoJian Y, Frauke F, Keith RF and Michael OE. Free radical scavenging activity, phenolic contents and cytotoxicity of selected Nigerian medicinal plants. Food Chemistry. 2012; 131:1279-1286.
  • Doll R. An overview of the epidemiological evidence linking diet and cancer. Proc. Nutr. Soc. 1990;49(2): 119-131.
  • Liu RH.. Potential synergy of phytochemicals in cancer prevention: mechanism of action. Journal of Nutrition. 2004; 134: 3475S-3489S.
  • Braun-Sprakties U..Amaranthus. In: Hager’s Handbuch der Pharmazeutischen Praxis, Band 4: Drogen AÐD (Hänsel R., Keller K., Rimpler H., and. Schneider G., eds.). Springer, BerlinÐHeidelbergÐ A New York, 1992; 239-241.
  • Cai Y Z, Sun M and Corke H.. Characterization and application of betalain pigments from plants of the Amaranthaceae. Trends in Food Science and Technology. 2005;16:370-376.
  • Cai Y, Sun M, and Corke H.. Identification and distribution of simple and acylated betacyanins in Amaranthaceae. J. Agric. Food Chem. 2001;49: 1971-1978.
  • Lim SD, Yusof YA, Chin N L, Talib T A, Endan J and Aziz MG.Effect of extraction parameters on the yield of betacyanins from pitaya fruit (Hylocereus Polyrhyzus) pulps. Journal of Food Agriculture and Environment . 2011;9:158-162.
  • Strack D, Vogt T and Schliemann W.“Recent advances in betalain research”. Phytochemistry . 2003;62: 247–269.
  • Wyler, Hugo and Ursula Meuer. "Zur Biogenese der Betacyane: Versuche mit [2‐14C]‐Dopaxanthin." Helvetica Chimica Acta . 1979; 62.4:1330-1339.
  • Piattelli M, Giudici de Nicole M, and Castrogiovanni V.Photocontrol of amaranthin synthesis in Amaranthus tricolor. Phytochemistry . 1969;8: 731-736
  • Schieber A, Keller P, Streker P and Klaiber I. Detection of isorhamnetin glycosides in [2-14C]-Dopaxanthin. Helv. HPLC-PDA and HPLC-APCI-MS/MS. Phytochem. Chim. Acta. 2002;62:1330-1339.
  • Cai Y Z, Sun M and Corke H.. Characterization and application of betalain pigments from plants of the Amaranthaceae. Trends in Food Science and Technology. 2005;16:370-376.
  • Stintzing F and Carle R. Betalains — emerging prospects for food scientists. Trends in Food Science and Technology. 2007;18:514–525.
  • Fernand W N and Adama Hilou. Phytochemical Composition, Antioxidant and Xanthine Oxidase Inhibitory Activities of Amaranthus cruentus L. and Amaranthus hybridus L. Pharmaceuticals.2012;5: 613-628
  • Schieber A, Keller P, Streker P and Klaiber I. Detection of isorhamnetin glycosides in [2-14C]-Dopaxanthin. Helv. HPLC-PDA and HPLC-APCI-MS/MS. Phytochem. Chim. Acta. 2002;62:1330-1339.
  • Cai Y, Sun M, and Corke H.. Identification and distribution of simple and acylated betacyanins in Amaranthaceae. J. Agric. Food Chem. 2001;49: 1971-1978.
  • Florian C, Stupien Grégory and Pascal Roullet. Involvement of the hippocampal CA3-region in acquisition and in memory consolidation of spatial but not in object information in mice. Neurobiology of learning and memory. 2003;80.1:32-41.
  • Tesoriere L, Butera DD, Arpa D, Di Gaudio F, Gentile C, and Livrea MA. Increased resistance to oxidation of betalainenriched human low density lipoproteins. Free Rad. Res. 2003;37:689-696.
  • Wettasinghe, Mahinda, Fereidoon Shahidi, and Ryszard Amarowicz. "Identification and quantification of low molecular weight phenolic antioxidants in seeds of evening primrose (Oenothera biennis L.)." Journal of Agricultural and Food Chemistry. 2002; 50.5:1267-1271.

Abstract Views: 26

PDF Views: 0




  • LCMS Spectral Analysis of Betacyanin Pigments in Amaranthus L.

Abstract Views: 26  |  PDF Views: 0

Authors

Pinkie Cherian
Depatment of Botany, St.Teresa’s College, Ernakulam, Kerala 682011, India
D. Sheela
Depatment of Botany, St.Teresa’s College, Ernakulam, Kerala 682011, India

Abstract


Plant pigments has got wider application in textile and pharmaceutical industry. Betacyanins are classes of purple plant pigments that are useful in plant identification and health monitoring. Pure samples of betacyanin were obtained by extracting and isolating the pigments found in Amaranthus L. plants. The resulting samples were then used to identify the absorption spectra of the pigment. The sample were analysed for measuring the absorbance at the range of 476nm and 538nm respectively. Betanin, Neobetanin and Amaranthine were found in the red pigments isolated from the plants which are of pharmaceutically important and got medicinal application. These pigments play important role as antioxidant and a natural dye for different formulations. The findings from the present spectral analysis showed 382 m/z ratio found to be derivative of betanidin component. LCMS analysis of the red pigment were carried out showing positive peak with 389 m/z ratio for betanidin compound among Amaranthus caudatus L. and Amaranthus dubius, Mart along with other peak values. The antioxidant capacity that is to scavenge the free radicals of these two Amaranthus L. species can be taken into account as it is a good dietary supplement in our daily life.

Keywords


Betacyanin, Betanin, Neobetalin , Amaranthine, LCMS.

References