Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Nitric Oxide Radical Scavenging Activity of the Ethanolic and Chloroform Extracts of Ocimum basilicum Linn. Leaves–A Comparative Study


Affiliations
1 Department of Pharmacognosy, SRM College of Pharmacy, Kattankulathur-603203, Tamilnadu, India
     

   Subscribe/Renew Journal


The aim of the present study is to investigate the efficiency of ethanolic and chloroform extracts of Ocimum basilicum Linn. leaves belonging to the family Labiatae for its in vitro antioxidant activity. The present study provides pharmacognostical, physicochemical and phytochemical details of the leaves of Ocimum basilicum which are useful in laying down standardization and pharmacopoeia parameters. In microscopic studies, transverse section (TS) of the leaves and its powder characters were studied and its characteristic features were established. Physicochemical parameters such as total ash value, acid insoluble ash value and water soluble ash value were determined. Preliminary phytochemical analysis of alcoholic extract and chloroform extract was carried out. The results of preliminary phytochemical screening were positive for flavonoids, carbohydrates, alkaloids, glycosides, proteins and saponin. The antioxidant potential of the chloroform and ethanol extract was evaluated using nitric oxide scavenging methods. It was found that the ethanolic extract showed better antioxidant effect compared to the chloroform extract.

Keywords

Ocimum basilicum, Pharmacognostic, Microscopy, Antioxidant, Nitric Oxide Scavenging.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Gupta, Prakash SJ, Srivastav S. Validation of Claim of Tulsi, Ocimum sanctum Linn as a Medicinal plant. Journal of Experimental Biology. 2002; 40:765-773.
  • Sen, P. Therapeutic Potential of Tulsi: From Experience to Facts. Drug News and Views. 1993; 1st ed: pp. 15-21.
  • Choudhury GB, Behera M, Jena PK, Tripathy SK. Pharmacognostical and Phytochemical Investigation of Various Tulsi plants Available in South eastern Odisha. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2011; 2(2): 605-610.
  • Sass JE, Elements of botanicals of microtechnique, New York, Mc Graw Hill and Co. 1998; pp. 222 – 225.
  • Quality control methods for medicinal plant materials WHO, Indian edition, Geneva. 2004; pp. 28-30.
  • Harborne, JB. Phytochemical methods, A guide to Modern Technique of Plant Analysis, Chapman and Hill, London. 1992.
  • Kadifkova panovska T, Kulevanova S, Marina stefova. In vitro antioxidant activity of some Teucrium species (Lamiaceae). Acta Pharm. 2005; 55:207-214.
  • Ganapathy S, Chandrasheka VM, Chitme HR, Lakashmi Narsu M. Antioxidant activity of gossypin and nevadensin. Indian Journal of Pharmacology. 2007;9(6):281-283.
  • Malinski T. Nitric oxide and nitroxidative stress in Alzheimer's disease. Journal of Alzheimer’s Disease. 2007;11:207-218.
  • Szabó C, Ischiropoulos H, Radi R. Peroxynitrite: Biochemistry, Pathophysiology and development of therapeutics. Drug discovery. 2007; 6: 662.
  • Burney S, Niles JC, Dedon PC, Tannenbaum SR. DNA damage in deoxynucleosides and oligonucleotides treated with peroxynitrite. Chemical Research in Toxicology. 1999; 12: 513‐520.
  • Rekha Rajendran, N Saleem Basha and S Ruby. Evaluation of In Vitro Antioxidant Activity of stem-bark and stem-wood of Premna serratifolia Lin., (Verbenaceae). Research journal of Pharmacognosy and Phytochemistry. 2009;1(1):11-14.
  • Nirmala Shinde, R.A. Saindane, S.K. Bhosale and Shreenivas Deshpande. In vitro Antioxidant Activity of Ethanolic and Chloroform Extracts of Tagetes erecta Flowers. Research journal of Pharmacognosy and Phytochemistry. 2010;2(4):324-326.
  • Njoku Obioma U., Joshua Parker Elijah and Okonkwo Chukwudi C. Antioxidant Properties of Gongronema latifolia. Asian Journal of Research Chemistry. 2011; 4(9): 1411-1414.
  • Dibyajyoti Saha* and Ankit Tamrakar. Xenobiotics, Oxidative Stress, Free Radicals vs. Antioxidants: Dance of Death to Heaven’s Life. Asian J. Res. Pharm. Sci. 1(2): April-June 2011; Page 36-38

Abstract Views: 23

PDF Views: 0




  • Nitric Oxide Radical Scavenging Activity of the Ethanolic and Chloroform Extracts of Ocimum basilicum Linn. Leaves–A Comparative Study

Abstract Views: 23  |  PDF Views: 0

Authors

P. R. Kumar
Department of Pharmacognosy, SRM College of Pharmacy, Kattankulathur-603203, Tamilnadu, India
S. Sakthi Priyadarsini
Department of Pharmacognosy, SRM College of Pharmacy, Kattankulathur-603203, Tamilnadu, India
M. Thirumal
Department of Pharmacognosy, SRM College of Pharmacy, Kattankulathur-603203, Tamilnadu, India

Abstract


The aim of the present study is to investigate the efficiency of ethanolic and chloroform extracts of Ocimum basilicum Linn. leaves belonging to the family Labiatae for its in vitro antioxidant activity. The present study provides pharmacognostical, physicochemical and phytochemical details of the leaves of Ocimum basilicum which are useful in laying down standardization and pharmacopoeia parameters. In microscopic studies, transverse section (TS) of the leaves and its powder characters were studied and its characteristic features were established. Physicochemical parameters such as total ash value, acid insoluble ash value and water soluble ash value were determined. Preliminary phytochemical analysis of alcoholic extract and chloroform extract was carried out. The results of preliminary phytochemical screening were positive for flavonoids, carbohydrates, alkaloids, glycosides, proteins and saponin. The antioxidant potential of the chloroform and ethanol extract was evaluated using nitric oxide scavenging methods. It was found that the ethanolic extract showed better antioxidant effect compared to the chloroform extract.

Keywords


Ocimum basilicum, Pharmacognostic, Microscopy, Antioxidant, Nitric Oxide Scavenging.

References