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Enzyme Immobilization: A Bridge of Understanding between Biotechnology and Pharmacy
As the demands of world biotechnology and pharmaceutical industries have increased, a need to develop and enhance, reaction stability, reusability and shelf life of enzymes has also become necessary. Hence noveltechnologies are needed to facilitate large-scale and economic formulations. Enzyme immobilization is a technique most apt to meet all the challenges in regards to enzyme activity. It provides immensescope for increasing availability of enzyme to the substrate and also increases product turn over, also increases the efficiency of an enzyme to a great extent. Immobilization of enzyme can be done by entrapment, support binding, cross-linking of enzyme crystals, etc. For immobilization, numerous natural and synthetic support materials are used. Nowadays, immobilized enzymes are much more preferred rather than their free counterpart because of their prolonged availability. Immobilized enzymesare widely used in pharmaceutical industries, cosmetic industries, food processing, biofuel production and many other sectors. The present study is performed for the motivation of the graduates towards publication and research. Hence, we have encouraged the graduates to prepare an informative article on the current subject.
Immobilization, Adsorption, Cross-Linking, Microencapsulation, Covalent Bonding.
- Mathiowitz E, Chickering D, Jacob JS, Santos C. Bioadhesive drug delivery systems. Encyclopedia of Controlled Drug Delivery. Vol. 1. New York: Wiley; 1999. p. 9-44.
- Hong X, Wei L, Ma L, Chen Y, Liu Z, Yuan W. Novel preparation method for sustained-release PLGA microspheres using water-inoilin-hydrophilic-oil-inwater emulsion. Int J Nanomedicine 2013; 8:2 433-41.
- Jorgensen L, Moeller EH, van de Weert M, Nielsen HM, Frokjaer S. Preparing and evaluating delivery systems for proteins. Eur J Pharm Sci 2006; 29: 174-82.
- Castellanos IJ, Cruz G, Crespo R, Griebenow K. Encapsulation induced aggregation and loss in activity of gamma-chymotrypsin and their prevention. J Control Release 2002;81:307-19.
- Ceriello A. The emerging role of post-prandial hyperglycaemic spikes in the pathogenesis of diabetic complications. Diabet Med 1998; 15: 188-93.
- Dabhi AS. Voglibose: An alpha-glucosidase inhibitor. Clin Diagn Res 2013; 7: 3023-7.
- Deruiter J. Overview of the antidiabetic agents. Endocr Pharmacother Module Spring 2003; 25: 1-33.
- Voglibose. Japanese Pharmacopeia. XV Official Monographs. 2006. p. 1231.
- Rao NM. Development and validation.
- SM Sarode, G Vidya Sagar, MK Kale, Nimase PK, Kulkarni AP, Firke SD,Firke BM, Warke PD, Chaudhari MA. Preparation and Evaluation of Floating Calcium Alginate Beads of Clarithromycin. Research J. Pharma. Dosage Forms and Tech. 2010; 2(2): 173-177.
- Kulkarni SJ. Enzyme immobilization: research and studies. Int J Res Rev. 2016; 3(7): 31-35.
- Aehle, W. Enzymes in Industry (Third edition), 2007 Wiley-VCH, ISBN 978-3-527-31689-2, Weinheim
- Literature Survey Methods in Biotechnology: Immobilization of Enzymes and Cells, 2006, 2nd (Ed.), 15-30.
- Costa, S. A.; Azevedo, H. S. and Reis, R. L. Enzymeimmobilization in biodegradable polymers for biomedical applications, In:Biodegradable systemsin tissue engineering and regenerative medicine. R.L. Reis and J. S. Román, (Ed.), 2005, CRC PressLLC, ISBN 978- 0-203-49123-2, London
- Guisan, J. M. Immobilization of enzymes as the 21st century begins, In: Immobilization of enzymesand cells. (Second edition), J. M. Guisan, (Ed.), 2009 Humana Press Inc., ISBN 1-597450537, New Jersey
- Sheldon, R. A. Enzyme immobilization: The quest for optimum performance. Advanced Synthesis and Catalysis. 2007, Vol. 349, No. 8-9, pp. 1289-1307, ISSN 1615-4169
- Cao, L. Carrier-bound immobilized enzymes, Principles, Application and Design (first edition), 2005, Wiley-VCH, ISBN 978-1-61583-208-8, Weinheim
- Hernandez, K. and Fernandez-Lafuente, R, Enzymeand Microbial Technology, 2011, Vol. 48, No. 2, pp. 107-122, ISSN 0141-0229
- Krajewska B. Enzyme and Microbial Technology,2004, Vol. 35, No. 2-3, pp. 126-139, ISSN 01410229
- Nelson J M, Griffin E G: Adsorption of Invertase, JAm Chem Soc 1916, 38: 1109-1115.
- N. Grubhofer and N Schelth , Nature, 1953, 4, 508.
- Harkins WD, Fourt L, Fourt PC. J Biol Chem 1940,132:111–118
- Gale EF, Epps MR, Biochem J 1944, 38:232–242
- Langmuir I, Schaefer VJ, J Am Chem Soc 1938, 60:1351–1360
- Persson M, Bulow L, Mosbach K, FEBS Lett 1990, 270:41–44 26. Wang P, Sergeeva MV, Lim L, Dordick JS. Nat Biotechnol 1997, 15:789–793
- Dordick JS, Novick SJ, Sergeeva MV. Chem Ind (London) 1998, 17–20
- .Matoba S, Tsuneda S, Saito K, Sugo T,Bio/Technology 1995, 13:795–797
- Kim J, H. Jia, C W. Lee, S W. Chung, Enz and Microb Technol. Vol 39, issue 3, 474-480, 2006
- Kim J, Grate J W. and P Wang. Nanobiocatalysis and its potential applications, 2008, cell press, 639-646, 10.1016.
- Wong LS, Thirlway J, Micklefield J. J. Am. Chem. Soc.2008 130(37): 12456-12464.
- Ghous T, Jn chem. Soc. Pak. Vol23, 4, 2001.
- Porath, J. and Axén, R. 1953: Methods in Enzymology, volume XLIV, (Mosbach, K., ed.), Academic Press, New York, NY, pp. 19-45.
- Chae, H.J. et al, Appl. Biochem. Biotechnol. 1998.73, 195.
- Quirk, R.A. et al, Biomaterials, 2001, 22, 865.
- Bernfeld P. and Wan J. Antigens and enzymes made insoluble by entrapping them into the lattices of synthetic polymers science 1963, 142, 678-679.
- Riaz A, Qader S, Anwar A, Iqbal S, Aust. J. Basicand Appl. 2009 Sci. 3, 2883.
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