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Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria


Affiliations
1 Department of Veterinary Microbiology, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
2 Department of Biochemistry, Usmanu Danfodiyo University, Sokoto, Nigeria
3 Department of Veterinary Services,Ministry of Animal Health and Fisheries Development, Usman Faruk Secretariate, Sokoto, Nigeria
 

This study was conducted to detect and characterize prevalent human group A rotavirus strains from 200 diarrheic children in Sokoto, Nigeria, by ELISA, monoclonal antibody (Mab) serotyping and Reverse Transcription-Polymerase Chain Reaction (RTPCR) techniques. Rotavirus was detected in 25.5% of the children. The G-serotypes observed in circulation were G4: 16 (59.3%), G1: 4 (14.8%), G2: 3 (11.1%), G3: 3 (11.1%), and G12: 1 (3.7%).The monoclonal antibody (Mab) serotyping detected G1 and G3 but did not detect G4 and G2 serotypes. TheMab typing of the G1 and G3 serotypes was consistent with the result of the RT-PCR. The VP4 genotypes detected were P[6] 3 (13%), P[8] 11 (47.8%), and the rare human P genotype (P[9]), found in 9 patients (39.1%). Nine strains identified with the common G and P combinations were G4 P[8] 5 (56%), G4 P[6] 1 (11%), G1 P[8] 2 (22%), and G3 P[8] 1 (11%), while seven strains with unusual combinations or rare G or P genotypes identified were G12 P[8] 1 (14%), G2 P[8] 2 (29%), and G4 P[9] 4 (57%). To our knowledge this is the first molecular study of human rotavirus and report of rare human G and P serotypes in Sokoto State.
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  • Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria

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Authors

B. R. Alkali
Department of Veterinary Microbiology, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
A. I. Daneji
Department of Veterinary Microbiology, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
A. A. Magaji
Department of Veterinary Microbiology, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
L. S. Bilbis
Department of Biochemistry, Usmanu Danfodiyo University, Sokoto, Nigeria
F. Bande
Department of Veterinary Services,Ministry of Animal Health and Fisheries Development, Usman Faruk Secretariate, Sokoto, Nigeria

Abstract


This study was conducted to detect and characterize prevalent human group A rotavirus strains from 200 diarrheic children in Sokoto, Nigeria, by ELISA, monoclonal antibody (Mab) serotyping and Reverse Transcription-Polymerase Chain Reaction (RTPCR) techniques. Rotavirus was detected in 25.5% of the children. The G-serotypes observed in circulation were G4: 16 (59.3%), G1: 4 (14.8%), G2: 3 (11.1%), G3: 3 (11.1%), and G12: 1 (3.7%).The monoclonal antibody (Mab) serotyping detected G1 and G3 but did not detect G4 and G2 serotypes. TheMab typing of the G1 and G3 serotypes was consistent with the result of the RT-PCR. The VP4 genotypes detected were P[6] 3 (13%), P[8] 11 (47.8%), and the rare human P genotype (P[9]), found in 9 patients (39.1%). Nine strains identified with the common G and P combinations were G4 P[8] 5 (56%), G4 P[6] 1 (11%), G1 P[8] 2 (22%), and G3 P[8] 1 (11%), while seven strains with unusual combinations or rare G or P genotypes identified were G12 P[8] 1 (14%), G2 P[8] 2 (29%), and G4 P[9] 4 (57%). To our knowledge this is the first molecular study of human rotavirus and report of rare human G and P serotypes in Sokoto State.