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SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha


Affiliations
1 Centre of Excellence in Biotechnology, M. P. Council of Science & Technology, Bhopal, Madhya Pradesh, India
2 Bacteriology Laboratory, Department of Post Graduate Studies & Research in Biological Science, Rani Durgawati University, Jabalpur, Madhya Pradesh, India
 

Commercially important Commiphora species are drought-tolerant plants and they are leafless for most of the year. Therefore, it is necessary to develop some molecular marker for the identification. Intended for that, in the present study, species-specific, sequence-characterized amplified regions (SCAR) markerswere developed for proficient and precise identification of closely related species Commiphora wightii and C. myrrha, which may ensure the quality, safety, and efficacy of medicines made from these plants through adulterous mixing of these plants. Two species-specific RAPD ampliconswere selected, gel-purified, cloned, and sequenced after screening of 20 RAPD primers.The sequence of 979 and 590 nucleotides (Genebank accession numbers K90051 and K90052) was used for development of 4 SCAR markers, namely, Sc1P, Sc1Pm, Sc2P, and Sc2Pm. Out of them, the Sc1Pm was specific for C. wightii, while Sc2P discriminated both the Commiphora species. These markers are first reported and will be useful for rapid identification of closely related Commiphora wightii and C. myrrha species.
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  • SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha

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Authors

Pramod Kumar Sairkar
Centre of Excellence in Biotechnology, M. P. Council of Science & Technology, Bhopal, Madhya Pradesh, India
Anjana Sharma
Bacteriology Laboratory, Department of Post Graduate Studies & Research in Biological Science, Rani Durgawati University, Jabalpur, Madhya Pradesh, India
N. P. Shukla
Centre of Excellence in Biotechnology, M. P. Council of Science & Technology, Bhopal, Madhya Pradesh, India

Abstract


Commercially important Commiphora species are drought-tolerant plants and they are leafless for most of the year. Therefore, it is necessary to develop some molecular marker for the identification. Intended for that, in the present study, species-specific, sequence-characterized amplified regions (SCAR) markerswere developed for proficient and precise identification of closely related species Commiphora wightii and C. myrrha, which may ensure the quality, safety, and efficacy of medicines made from these plants through adulterous mixing of these plants. Two species-specific RAPD ampliconswere selected, gel-purified, cloned, and sequenced after screening of 20 RAPD primers.The sequence of 979 and 590 nucleotides (Genebank accession numbers K90051 and K90052) was used for development of 4 SCAR markers, namely, Sc1P, Sc1Pm, Sc2P, and Sc2Pm. Out of them, the Sc1Pm was specific for C. wightii, while Sc2P discriminated both the Commiphora species. These markers are first reported and will be useful for rapid identification of closely related Commiphora wightii and C. myrrha species.