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Microsatellite DNA Marker Aided Diversity Analysis in Frenchbean (Phaseolus vulgaris L.)


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1 Sunflower Seed Production Scheme, Main Research Station, Hebbal, Bangalore 560 024, India
     

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Sixty four french bean genotypes were analyzed with 15 microsatellite markers to test variation. Among the total 15 SSR primers used, 10 primers were polymorphic and 5 were monomorphic. Of the 15 SSR primers used, 10 primer pairs showed polymorphism. The number of alleles produced by different primers ranged from two to four. The high level of polymorphism (66.66%) was obtained. The cluster construction based on SSR marker data revealed significant genetic variation among the genotypes. SSR marker system showed high dissimilarity among the french bean genotypes. Cluster diagram constructed using polymorphic markers identified two major clusters. All exotic collections, landraces, local types and released varieties were grouped in same cluster with some indigenous collection, whereas cluster A comprised only of indigenous collection.

Keywords

Dendrogram, Breeding, French Bean, Germplasm, Microsatellite, Polymorphism.
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  • Microsatellite DNA Marker Aided Diversity Analysis in Frenchbean (Phaseolus vulgaris L.)

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Authors

Nishimwe Immaculee
Sunflower Seed Production Scheme, Main Research Station, Hebbal, Bangalore 560 024, India
M. S. Uma
Sunflower Seed Production Scheme, Main Research Station, Hebbal, Bangalore 560 024, India
Niranjana Hegde
Sunflower Seed Production Scheme, Main Research Station, Hebbal, Bangalore 560 024, India
T. E. Nagaraja
Sunflower Seed Production Scheme, Main Research Station, Hebbal, Bangalore 560 024, India
Y. M. Somashekahar
Sunflower Seed Production Scheme, Main Research Station, Hebbal, Bangalore 560 024, India

Abstract


Sixty four french bean genotypes were analyzed with 15 microsatellite markers to test variation. Among the total 15 SSR primers used, 10 primers were polymorphic and 5 were monomorphic. Of the 15 SSR primers used, 10 primer pairs showed polymorphism. The number of alleles produced by different primers ranged from two to four. The high level of polymorphism (66.66%) was obtained. The cluster construction based on SSR marker data revealed significant genetic variation among the genotypes. SSR marker system showed high dissimilarity among the french bean genotypes. Cluster diagram constructed using polymorphic markers identified two major clusters. All exotic collections, landraces, local types and released varieties were grouped in same cluster with some indigenous collection, whereas cluster A comprised only of indigenous collection.

Keywords


Dendrogram, Breeding, French Bean, Germplasm, Microsatellite, Polymorphism.

References