Open Access Open Access  Restricted Access Subscription Access

Cloning and Expression of Ama r 1, as a Novel Allergen of Amaranthus retroflexus Pollen


Affiliations
1 Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran, Islamic Republic of
 

Sensitisation to Amaranthus retroflexus pollen is very common in tropical and subtropical countries. In this study we aimed to produce a recombinant allergenic Ole e 1-like protein from the pollen of this weed. To predict cross-reactivity of this allergen (Ama r 1) with other members of the Ole e 1-like protein family, the nucleotide sequence homology of the Ama r 1 was investigated.The expression of Ama r 1 in Escherichia coli was performed by using a pET-21b(+) vector.The IgE-binding potential of recombinant Ama r 1 (rAma r 1) was evaluated by immunodetection and inhibition assays using 26 patients' sera sensitised to A. retroflexus pollen. The coding sequence of the Ama r 1 cDNA indicated an open reading frame of 507 bp encoding for 168 amino acid residues which belonged to the Ole e 1-like protein family. Of the 26 serum samples, 10 (38.46%) had significant specific IgE levels for rAma r 1. Immunodetection and inhibition assays revealed that the purified rAma r 1 might be the same as that in the crude extract. Ama r 1, the second allergen from the A. retroflexus pollen, was identified as a member of the family of Ole e 1-like protein.
User
Notifications
Font Size

Abstract Views: 50

PDF Views: 1




  • Cloning and Expression of Ama r 1, as a Novel Allergen of Amaranthus retroflexus Pollen

Abstract Views: 50  |  PDF Views: 1

Authors

Payam Morakabati
Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran, Islamic Republic of
Mohammad-Ali Assarehzadegan
Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran, Islamic Republic of
Gholam Reza Khosravi
Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran, Islamic Republic of
Bahareh Akbari
Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran, Islamic Republic of
Fatemeh Dousti
Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran, Islamic Republic of

Abstract


Sensitisation to Amaranthus retroflexus pollen is very common in tropical and subtropical countries. In this study we aimed to produce a recombinant allergenic Ole e 1-like protein from the pollen of this weed. To predict cross-reactivity of this allergen (Ama r 1) with other members of the Ole e 1-like protein family, the nucleotide sequence homology of the Ama r 1 was investigated.The expression of Ama r 1 in Escherichia coli was performed by using a pET-21b(+) vector.The IgE-binding potential of recombinant Ama r 1 (rAma r 1) was evaluated by immunodetection and inhibition assays using 26 patients' sera sensitised to A. retroflexus pollen. The coding sequence of the Ama r 1 cDNA indicated an open reading frame of 507 bp encoding for 168 amino acid residues which belonged to the Ole e 1-like protein family. Of the 26 serum samples, 10 (38.46%) had significant specific IgE levels for rAma r 1. Immunodetection and inhibition assays revealed that the purified rAma r 1 might be the same as that in the crude extract. Ama r 1, the second allergen from the A. retroflexus pollen, was identified as a member of the family of Ole e 1-like protein.