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Isolation, Identification and Drug Resistance Patterns of Methicillin Resistant Staphylococcus aureus from Mastitic Cow's Milk from Selected Dairy Farms in and around Kombolcha, Ethiopia


Affiliations
1 College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
 

A cross sectional study was conducted between January and May 2015 in and around Kombolcha town, Eastern Amhara, to identify methicillin resistant Staphylococcus aureus and their resistance to antimicrobials and also to identify risk factors associated with the occurrence of dairy cow mastitis. A total of 150 dairy cows were included during the study period. A total of 600 quarters were examined to detect clinical and subclinical mastitis by physical examinations of udder and milk and California mastitis test, respectively. The prevalence of mastitis was 56%. Age, milking hygiene, parity, and floor system had significant (p<0.05) effect on the prevalence of mastitis. S. aureus was isolated at a rate of 11 (73.3%) and 29 (42%) in clinical and subclinical mastitis, respectively. The result showed the occurrence of S. aureus in clinical mastitis was found to be significantly higher than subclinical mastitis (p=0.028). The present study showed that S. aureus was resistant to penicillin G (100%), amoxicillin (100%), cefoxitin (42.7%) and tetracycline (77.4%) however all the isolates were found to be totally (100%) susceptible to the gentamycin. 45.3% of the isolates were found to be multidrug resistant. Antimicrobial resistance is due to repeated therapeutic use of drugs. Regular antimicrobial sensitivity testing helps to select effective antibiotics that ultimately reduce the development of resistance towards commonly used antibiotics. In conclusion, Mastitis caused by S. aureus is one of the major problems of dairy cows in milk production in the study area.

Keywords

Antimicrobials, Mastitic Cows, Methicillin-Resistant Staphylococcus aureus, Prevalence, Risk Factors.
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  • Isolation, Identification and Drug Resistance Patterns of Methicillin Resistant Staphylococcus aureus from Mastitic Cow's Milk from Selected Dairy Farms in and around Kombolcha, Ethiopia

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Authors

Asmelash Tassew
College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
Mesfin Negash
College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
Addisu Demeke
College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
Aklilu Feleke
College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
Biruk Tesfaye
College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
Tesfaye Sisay
College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia

Abstract


A cross sectional study was conducted between January and May 2015 in and around Kombolcha town, Eastern Amhara, to identify methicillin resistant Staphylococcus aureus and their resistance to antimicrobials and also to identify risk factors associated with the occurrence of dairy cow mastitis. A total of 150 dairy cows were included during the study period. A total of 600 quarters were examined to detect clinical and subclinical mastitis by physical examinations of udder and milk and California mastitis test, respectively. The prevalence of mastitis was 56%. Age, milking hygiene, parity, and floor system had significant (p<0.05) effect on the prevalence of mastitis. S. aureus was isolated at a rate of 11 (73.3%) and 29 (42%) in clinical and subclinical mastitis, respectively. The result showed the occurrence of S. aureus in clinical mastitis was found to be significantly higher than subclinical mastitis (p=0.028). The present study showed that S. aureus was resistant to penicillin G (100%), amoxicillin (100%), cefoxitin (42.7%) and tetracycline (77.4%) however all the isolates were found to be totally (100%) susceptible to the gentamycin. 45.3% of the isolates were found to be multidrug resistant. Antimicrobial resistance is due to repeated therapeutic use of drugs. Regular antimicrobial sensitivity testing helps to select effective antibiotics that ultimately reduce the development of resistance towards commonly used antibiotics. In conclusion, Mastitis caused by S. aureus is one of the major problems of dairy cows in milk production in the study area.

Keywords


Antimicrobials, Mastitic Cows, Methicillin-Resistant Staphylococcus aureus, Prevalence, Risk Factors.