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Tick Burden and Acquisition of Immunity to Theileria parva by Tarime Cattle in Comparison to Sukuma Cattle under Different Tick Control Regimes in the Lake Zone of Tanzania


Affiliations
1 Department of Animal Science and Production, Sokoine University of Agriculture (SUA), P. O. Box 3004, Chuo Kikuu Morogoro, Tanzania, United Republic of
2 Department of Animal Science and Production, SUA, P. O. Box 3004, Chuo Kikuu Morogoro, Tanzania, United Republic of
3 Department of Veterinary Medicine and Public Health, SUA, P. O. Box 3021, Chuo Kikuu Morogoro, Tanzania, United Republic of
4 Department of Veterinary Anatomy, SUA, P. O. Box 3016, Chuo Kikuu Morogoro, Tanzania, United Republic of
5 Tanzania Livestock Research Institute Mabuki, P. O. Box 352, Mwanza, Tanzania, United Republic of
6 Nelson Mandela Institute of Science and Technology, P. O. Box 447, Arusha, Tanzania, United Republic of
7 Genome Science Centre, SUA, P. O. Box 3016, Chuo Kikuu Morogoro, Tanzania, United Republic of
 

This study was conducted to determine tick burden and immunological parameters of resistance to East Coast fever (ECF) in Tarime and Sukuma cattle. Tick load, packed cell volume (PCV), Theileria parva (T. parva) specific antibody percent positivity (PP), and prevalence of T. parva parasites were studied in relation to dipping regime, strains, and season. A total of 50 experimental cattle were included in this study. Tick load was determined by whole body counts, antibody percent positivity was determined by the polymorphic immunodominant molecule (PIM)-based T. parva enzyme-linked immunosorbent assay (ELISA), and prevalence of T. parva parasites was detected by a nested polymerase chain reaction (PCR) based on the p104 gene. Dipping frequency on tick burden showed no statistically significant differences when cattle of either strain were dipped either once every 2 or 3 weeks in the dry and wet seasons. However, Tarime cattle had higher (p<0.05) tick count than Sukuma cattle and non dipped groups maintained high tick infestation throughout the experimental period. The PCV values were within the physiological range, although this parameter was lower in Tarime cattle (p<0.05). All cattle regardless of strain were seropositive, although Tarime cattle maintained higher PP compared to Sukuma by 15%. Conversely, the prevalence of T. parva parasites was lower in Tarime (38%) compared to Sukuma cattle (38.5%), but the difference was not significant (p>0.05). During the study period, 20% (5/25) of Sukuma cattle contracted ECF, but none of the Tarime cattle showed clinical signs for the disease. The differences between the two strains shown in terms of PP and T. parva parasite prevalence may indicate the ability of individual cattle to resist tick infestation and ECF infection under natural challenge. Higher antibody levels but lower parasite prevalence attained by Tarime cattle, suggests inherent ability of Tarime cattle to resist clinical development of ECF infection, but to remain as T. parva carriers.

Keywords

Carrier State, Theileria parva, Ticks, Seropositivity.
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  • Tick Burden and Acquisition of Immunity to Theileria parva by Tarime Cattle in Comparison to Sukuma Cattle under Different Tick Control Regimes in the Lake Zone of Tanzania

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Authors

E. L. K. Laisser
Department of Animal Science and Production, Sokoine University of Agriculture (SUA), P. O. Box 3004, Chuo Kikuu Morogoro, Tanzania, United Republic of
S. W. Chenyambuga
Department of Animal Science and Production, SUA, P. O. Box 3004, Chuo Kikuu Morogoro, Tanzania, United Republic of
E. D. Karimuribo
Department of Veterinary Medicine and Public Health, SUA, P. O. Box 3021, Chuo Kikuu Morogoro, Tanzania, United Republic of
G. Msalya
Department of Animal Science and Production, SUA, P. O. Box 3004, Chuo Kikuu Morogoro, Tanzania, United Republic of
M. J. Kipanyula
Department of Veterinary Anatomy, SUA, P. O. Box 3016, Chuo Kikuu Morogoro, Tanzania, United Republic of
A. J. Mwilawa
Tanzania Livestock Research Institute Mabuki, P. O. Box 352, Mwanza, Tanzania, United Republic of
R. H. Mdegela
Department of Veterinary Medicine and Public Health, SUA, P. O. Box 3021, Chuo Kikuu Morogoro, Tanzania, United Republic of
L. J. M. Kusiluka
Nelson Mandela Institute of Science and Technology, P. O. Box 447, Arusha, Tanzania, United Republic of
P. S. Gwakisa
Genome Science Centre, SUA, P. O. Box 3016, Chuo Kikuu Morogoro, Tanzania, United Republic of

Abstract


This study was conducted to determine tick burden and immunological parameters of resistance to East Coast fever (ECF) in Tarime and Sukuma cattle. Tick load, packed cell volume (PCV), Theileria parva (T. parva) specific antibody percent positivity (PP), and prevalence of T. parva parasites were studied in relation to dipping regime, strains, and season. A total of 50 experimental cattle were included in this study. Tick load was determined by whole body counts, antibody percent positivity was determined by the polymorphic immunodominant molecule (PIM)-based T. parva enzyme-linked immunosorbent assay (ELISA), and prevalence of T. parva parasites was detected by a nested polymerase chain reaction (PCR) based on the p104 gene. Dipping frequency on tick burden showed no statistically significant differences when cattle of either strain were dipped either once every 2 or 3 weeks in the dry and wet seasons. However, Tarime cattle had higher (p<0.05) tick count than Sukuma cattle and non dipped groups maintained high tick infestation throughout the experimental period. The PCV values were within the physiological range, although this parameter was lower in Tarime cattle (p<0.05). All cattle regardless of strain were seropositive, although Tarime cattle maintained higher PP compared to Sukuma by 15%. Conversely, the prevalence of T. parva parasites was lower in Tarime (38%) compared to Sukuma cattle (38.5%), but the difference was not significant (p>0.05). During the study period, 20% (5/25) of Sukuma cattle contracted ECF, but none of the Tarime cattle showed clinical signs for the disease. The differences between the two strains shown in terms of PP and T. parva parasite prevalence may indicate the ability of individual cattle to resist tick infestation and ECF infection under natural challenge. Higher antibody levels but lower parasite prevalence attained by Tarime cattle, suggests inherent ability of Tarime cattle to resist clinical development of ECF infection, but to remain as T. parva carriers.

Keywords


Carrier State, Theileria parva, Ticks, Seropositivity.