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Isolation and Identification Studies on Potential Xylanase Producing Strain Trichoderma sp. WICC F46 Isolated from Tropical Soil


Affiliations
1 Insitute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
2 Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat University, Sadat City,, Egypt
3 Department of Microbiology, PSGVP Mandal’s, Arts, Science, and Commerce College, Shahada 425 409, Maharashtra, India
 

Trichoderma is one of few fungal species of high potential application as bio factory for xylanase production. During last few years increased demand have been observed on the need to isolate high efficient strains from soil sample and to use different microbiological and bioprocess engineering approaches to increase the production yield. In this work, efficient xylanase producer strain was isolated from soil and fully identified using phylogeny analysis. In order to develop an efficient submerged medium for xylanase production, media screening was carried out followed by optimization using one factor at a time (OFAT) method. After optimization of medium composition, the maximal xylanase volumetric production reached 311.71 UmL-1. This medium was composed of corn cob, peptone, magnesium sulphate, calcium chloride, and ammonium sulphate.

Keywords

Trichoderma, Strain Isolation, Xylanase, Medium Optimization, Submerged Culture.
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  • Isolation and Identification Studies on Potential Xylanase Producing Strain Trichoderma sp. WICC F46 Isolated from Tropical Soil

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Authors

K. K. Ambehabati
Insitute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
S. Z. Hanapi
Insitute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
A. F. El Baz
Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat University, Sadat City,, Egypt
R. Z. Sayyed
Department of Microbiology, PSGVP Mandal’s, Arts, Science, and Commerce College, Shahada 425 409, Maharashtra, India
D. J. Dailin
Insitute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
H. A. El Enshasy
Insitute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia

Abstract


Trichoderma is one of few fungal species of high potential application as bio factory for xylanase production. During last few years increased demand have been observed on the need to isolate high efficient strains from soil sample and to use different microbiological and bioprocess engineering approaches to increase the production yield. In this work, efficient xylanase producer strain was isolated from soil and fully identified using phylogeny analysis. In order to develop an efficient submerged medium for xylanase production, media screening was carried out followed by optimization using one factor at a time (OFAT) method. After optimization of medium composition, the maximal xylanase volumetric production reached 311.71 UmL-1. This medium was composed of corn cob, peptone, magnesium sulphate, calcium chloride, and ammonium sulphate.

Keywords


Trichoderma, Strain Isolation, Xylanase, Medium Optimization, Submerged Culture.

References