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Singh, Tanuja
- Biodegradation of Pesticide Chlorpyrifos by Bacteria Staphylococcus aureus (Accession no. CP023500.1) Isolated from Agricultural Soil
Abstract Views :362 |
PDF Views:194
Authors
Affiliations
1 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, IN
2 Department of Biotechnology and Botany, TPS College, Patna – 800001, Bihar, IN
3 Department of Biochemistry, Magadh University, Bodh Gaya – 824234, Bihar, IN
4 Department of Zoology, Babasaheb Bhimrao Ambedkar Bihar University, Muzaffarapur – 842002, Bihar, IN
1 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, IN
2 Department of Biotechnology and Botany, TPS College, Patna – 800001, Bihar, IN
3 Department of Biochemistry, Magadh University, Bodh Gaya – 824234, Bihar, IN
4 Department of Zoology, Babasaheb Bhimrao Ambedkar Bihar University, Muzaffarapur – 842002, Bihar, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 20, No 1&2 (2020), Pagination: 21-26Abstract
The use of pesticides like Chlorpyrifos in agricultural soil is the primary reason for the pollution of aquatic and terrestrial environments. Today the most effective method used for bioremediation are by using microbes. Different pesticide degrading bacteria were isolated and identified by the mean of cultural, biochemical tests and which is further identified and confirmed by 16S RNA sequencing method. The most potent strain S-1 growth in mineral salt medium supplemented with Chlorpyrifos as sole source of carbon (50 to 1000 ug/ml) its optical density was measured at 600 nm. The bacterial growth is optimised on the parameter of different physiochemical condition were. The result showed that S. aureus shows maximum growth on 12th day. The HPLC analysis was also done for calculating the residual percentage of Chlorpyrifos after 12 days incubation which showed that S. aureus was able to degrade 99% of the pesticide of the 1000 ug/ml CP concentration in the MSM. The results of this research shows that the isolated bacteria have the potential to be used in bioremediation of Chlorpyrifos contaminated soil and water ecosystems.
Keywords
Bacterial, Bioremediation, Chlorpyrifos, Degradation, Pesticide.Full Text
References
- Sachdeva S. Pesticides and their socio-economic impact on agriculture. South. Econ. 2007; 41(38):42–53. https://doi.org/10.1016/j.seps.2005.04.002
- Fulekar MH. UGC major research project development of bioremediation technology for pesticide industrial wastes using novel cow dung microorganisms in sequence biological reactor and two phase partitioning bioreactor. 2008.
- Chang TC, Chang HC, Yu FW, Leni D, Mario V, Chung T. Species level identification of isolates of the Acinetobacter calcoaceticus Acinetobacter baumanni complex by sequence analysis of the 16523SrRNA gene spacer region. J Clin Microbiol. 2005; 43(4):1632–9.PMid: 15814977 PMCid: PMC1081347. https://doi.org/10.1128/JCM.43.4.1632-1639.2005
- Geetha M, Fulekar MH. Bioremediation of pesticides in surface soil treatment unit using microbial consortia. Afr J Environ Sci Technol. 2008; 2(2):36–45.
- Zhu J, Zhao Y, Qiu J. Isolation and application of a Chlorpyrifosdegrading Bacillus licheniformis ZHU-1. Afr J Microbiol Res.2010; 4:2410–3.
- Buchanna RE, Gibbons NE. Bergey’s manual of determinative bacteriology. 8th ed. Baltimore: Williams and Wilkins; 1984.
- Aneja KR. Experiments in microbiology, plant pathology and biotechnology. Fourth ed. New Age International Limited. 2013.
- Alexander M. Biodegradation and bioremediation. ed. 2 M.Alexander. San Diego: Academic Press; 1999.
- Yang T, Ren L, Jia Y, Fan S, Wang J, Wang J, Nahurira R, Wang H, Yan Y. Biodegradation of Di-(2-ethylhexyl) Phthalate by Rhodococcus ruber YC-YT1 in contaminated water and soil.Int Journal of Environ Re. Public Health. 2018. p. 15–35. PMid: 29751654 PMCid: PMC5982003. https://doi.org/10.3390/ijerph15050964
- Edgar RC. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res.2004; 32(5):1792–7. PMid: 15034147 PMCid: PMC390337.https://doi.org/10.1093/nar/gkh340
- Talavera G, Castresana J. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology. 2007; 56:564–77.PMid: 17654362. https://doi.org/10.1080/10635150701472164
- Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O. Phylogeny.f r: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008. p. 1:36. PMid: 18424797 PMCid: PMC2447785. https://doi.org/10.1093/nar/gkn180
- Singh BK, Walker A, Morgan JAW, Wright DJ. Biodegradation of Chlorpyrifos by Enterobacter strain B-14 and its use in biodegradation of contaminated soils. Applied and Environmental Microbiology. 2004; 70:4855–63. PMid: 15294824 PMCid: PMC492451. https://doi.org/10.1128/AEM.70.8.4855-4863.2004
- Vijayalakshmi P, Usha MS. Optimization of Chlorpyrifos degradation by Pseudomonas putida. J Chem Pharm. 2012; 4:2532–9.
- Li X, He J, Li S. Isolation of Chlorpyrifos-degrading bacterium, Sphingomonas sp. strain Dsp-2 and cloning of the mpd gene.Research in Microbiology. 2007; 158:143–9. PMid: 17306510.https://doi.org/10.1016/j.resmic.2006.11.007
- Fulekar MH. Bioremediation technologies for environment.Indian J Environ Protect. 2005; 25(4):358–64.
- Rani SM, Lakshim KV, Devi SP, Madlian F, Aruna K. Isolation and characterization of Chlorpyrifos degrading bacteria from agricultural soil and its growth response. African Journal of Microbiology. 2008; 2:26–31.
- Anwar S, Liaquat F, Khan QM, Khalid ZM, Iqbal S. Biodegradation of Chlorpyrifos and its hydrolysis product 3,5,6- trichloro-2pyridinol by Bacillus pumilus strain C2A1. J. Hazard. Mater.2009; 168(1):400–5. PMid: 19297093. https://doi.org/10.1016/j.jhazmat.2009.02.059
- Potential of Bacillus cereus (Accession number KY7506901) on Di (2-Ethylhexyl) Phthalate Degradation and its Characterization by LCMS
Abstract Views :269 |
PDF Views:100
Authors
Affiliations
1 Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, IN
1 Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 20, No 1&2 (2020), Pagination: 114-119Abstract
The present work has been undertaken for remediating phthalate exposure in the environment. The microbial strain was isolated by enrichment culture technique from the rubbish dump space close to Patna that was contaminated with phthalates for higher degradation ability. The isolated microbial strain T7 was designated as Bacillus cereus after Gram-staining, biochemical characterization, 16S-rRNA sequence and phylogenetic studies. The isolate had the power to utilize 25O μg/ml Di (2-ethyl Hexyl Phthalate) (DEHP) dose taken from 10 mg/ml (DEHP) stock solution within the growth medium. The optimum pH and temperature for DEHP degradation were 8.5 at 37 ̊C. The isolated bacterial strain T7 may allow up to 10% NaCl in minimal salt medium that was enrich with DEHP. The metabolic end product obtained after LCMS was bis [3-(oxolan-2-yl) propyl] nonanedioate having chemical formula C23H40O6. This work provides some new proof for soil rectification by Bacillus species.
Keywords
Biochemical characterization, Biodegradation, DEHP, 16sr-RNA, LC-MS.Full Text
References
- Chang BV, Yang CM, Cheng CH, Yuan SY. Biodegradation of phthalate esters by two bacteria strains. Chemosph.2004; 55:533–8. PMid: 15006506. https://doi.org/10.1016/j.chemosphere.2003.11.057
- Latorre I, Hwang S, Sevillano, M, Montalvo-Rodriguez R. PVC biodeterioration and DEHP leaching by DEHP-degrading bacteria. Int Biodeterior Biodegr. 2012b; 69:73–81. PMid: 22736894 PMCid: PMC3377483. https://doi.org/10.1016/j.ibiod.2011.12.011
- Lomenick JP, Calafat AM, Castro MSM, Mier R, Stenger P, Foster MB, et. al. Phthalate exposure and precocious puberty in females. J Pediatr. 2010; 156:221–5. PMid: 19892364.https://doi.org/10.1016/j.jpeds.2009.09.047
- Lo D, Wang YT, Wu MC. Hepatoprotective effect of silymarin on Di (2-ethylhexyl) Phthalate (DEHP) induced injury in liver FL83B cells. Environ Toxicol Pharmacol. 2014; 38:112–8.PMid: 24934613. https://doi.org/10.1016/j.etap.2014.05.005 5.
- Zong T, Lai L, Hu J, Guo M, Li M, Zhang L, et al. Maternal exposure to Di-(2-ethylhexyl) Phthalate disrupts placental growth and development in pregnant mice. J Hazard Mater.2015; 297:25-33. PMid: 25935407. https://doi.org/10.1016/j.jhazmat.2015.04.065
- Chen H, Zhang W, Rui BB, Yang SM, Xu WP. Di (2-ethylhexyl) Phthalate exacerbates non-alcoholic fatty liver in rats and its potential mechanisms. Environ Toxicol Pharmacol. 2016; 42:38– 44. PMid: 26773359. https://doi.org/10.1016/j.etap.2015.12.016
- Net S, Delmont A, Sempere R, Paluselli A, Ouddane B. Reliable quantification of phthalates in environmental matrices (air, water, sludge, sediment and soil): A review. Science of the Total Environment, 2015; 515-516:162–80. PMid: 25723871. https:// doi.org/10.1016/j.scitotenv.2015.02.013
- Mankidy R, Wiseman S, Ma Hong, Giesy JP. Biological impact of phthalates. Toxicol Letters. 2013; 2171:5-58. PMid: 23220035.https://doi.org/10.1016/j.toxlet.2012.11.025
- 9. Magdouli S, Daghrir R, Brar SK, Drogui P, Tyagi RD. Di 2-ethylhexyl Phthalate in the aquatic and terrestrial environment: a critical reWview J Environ Manage. 2013; 127:36-49. PMid: 23681404. https://doi.org/10.1016/j.jenvman.2013.04.013
- Baek JH, Gu MB, Sang BI, Kwack SJ, Kim KB, Lee BM. Risk reduction of adverse effects due to Di-(2-ethylhexyl) Phthalate (DEHP) by utilizing microbial degradation. J Toxicol Environ Health, Part A. 2009; 72:138–94. PMid: 20077210. https://doi.org/10.1080/15287390903212733
- Meng X, Niu G, Yang W, Cao X. Di (2-ethylhexyl) Phthalate biodegradation and denitrification by a Pseudoxanthomonas sp. strain. Bioresour Technol. 2015; 180:356–9. PMid: 25596917.https://doi.org/10.1016/j.biortech.2014.12.071
- Pradeep S, Josh MS, Binod P, Devi RS, Balachandran S, Anderson RC, et al. Achromobacter denitrificans strain SP1 efficiently remediates Di (2-ethylhexyl) Phthalate. Ecotoxicol Environ Saf.2015a; 112:114–21. PMid: 25463861. https://doi.org/10.1016/j.ecoenv.2014.10.035
- Aneja KR. Experiments in Microbiology plant pathology and Biotechnology Fourth ed. New Delhi: New Age International (P) Ltd, Publishers; 2007; 157:245–75.
- Edgar RC. MUSCLE multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004; 32:1792–7. PMid: 15034147 PMCid: PMC390337. https://doi.org/10.1093/nar/gkh340
- Talavera G, Castresana J. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Bio. 2007; 56:564–77.PMid: 17654362. https://doi.org/10.1080/10635150701472164
- Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O. Phylogeny for robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008; 36(S2):W465–9. PMid: 18424797 PMCid: PMC2447785, https://doi.org/10.1093/nar/gkn180
- Prasad B, Suresh S. Biodegradation of Phthalate esters by Variovorax sp. Sciverse Science Direct. APCBEE Procedia. 2012; 1:16–21. https://doi.org/10.1016/j.apcbee.2012.03.004
- Jin D, Kong X, Cui B, Bai Z, Zhang H. Biodegradation of Di-nbuty Phthalate by bacterial Consortium LV-1 enriched from river sludge. 2017.
- Biodegradation of Di-(2-Ethylhexyl Phalate) by Bacillus antracis (Accession no. KJ085972.1)
Abstract Views :233 |
PDF Views:98
Authors
Affiliations
1 TPS College, Patna – 800001, Bihar, IN
2 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, IN
3 DST-WOS, TPS College, Patna – 800001, Bihar, IN
1 TPS College, Patna – 800001, Bihar, IN
2 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, IN
3 DST-WOS, TPS College, Patna – 800001, Bihar, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 20, No 3&4 (2020), Pagination: 140-144Abstract
DEHP is one of the most broadly used PAEs (Phthalic Acid Esters) as a plasticizer in Polyvinyl Chloride (PVC) manufacturing. The DEHP and its other monoester metabolites are considered as very harmful for animal and human and affect the endocrine system. The strain isolated in this study is very efficient in degrading the DEHP. The degrading bacterial strain Bacillus anthracis is used in this study to determine the biological degradation potential of DEHP. Bacteria play very important roles in DEHP degradation in the environment under various conditions. The selected strain in for DEHP degrading bacteria designated as strain T-10 is optimized at the different conditions for its maximum activity at different temperatures, pH, chemicals like carbon and nitrogen source identified with degradation potential of more than 85%.
Keywords
Bacteria, Biodegradation, DEHP, PlasticizerFull Text
References
- Sant KE, Dolinoy DC, Jilek JL, Sartor MA, Harris C. Mono-2ethylhexyl phthalate disrupts neurulation and modifies the embryonic redox environment and gene expression. Journal of Reprod Toxicol. 2016; 63:32–48. PMid: 27167697 PMCid: PMC4987184. https://doi.org/10.1016/j.reprotox.2016.03.042
- Nielsen E, Larsen PB. Toxicological evaluation and limit values for DEHP and phthalates other than DEHP. Environmental Review Report 6. Copenhagen, Denmark: Danish Environmental Protection Agency; 1996.
- Ribbons DW, Keyser P, Kunz DA, Taylor BF. Microbial degradation of phthalates. Gibson, D.T., ed. Microbial degradation of organic compounds. New York: Dekker; 1984.
- Sugatt RH, Ogrady DP, Banerjee S, Howard PH, Gledhill WE. Shake flask biodegradation of 14 commercial phthalate esters. Journal of Appl Environ Microbiol. 1984; 47:601–6. PMid: 16346500 PMCid: PMC239733. https://doi.org/10.1128/AEM.47.4.601-606.1984
- Nozawa T, Maruyama Y. Anaerobic metabolism of phthalate and other aromatic-compounds by a denitrifying bacterium. Journal of Bacteriol. 1988; 170:5778–84. PMid: 3192515 PMCid: PMC211682. https://doi.org/10.1128/JB.170.12.57785784.1988
- Staples CA, Peterson DR, Parkerton TF, Adams WJ. The environmental fate of phthalate esters: A literature review. Journal of Chemosphere. 1997; 35:667–749. https://doi.org/10.1016/S0045-6535(97)00195-1
- Buchanna RE, Gibbons NE. Bergey’s manual of determinative bacteriology. 8th ed. Baltimore: Williams and Wilkins; 1984.
- Tariq M. Isolation and screening of azo dye decolorizing bacterial isolates from dye contaminated textile wastewater. Journal of Soil Environ. 2011; 30(1):7–12.
- Yang T, Ren L, Jia Y, Fan S, Wang J, Wang J, Nahurira R, Wang H, Yan Y. Biodegradation of Di-(2-ethylhexyl) Phthalate by Rhodococcus ruber YC-YT1 in Contaminated Water and Soil. Int Journal of Environ Res Public Health. 2018; 15(5):964. PMid: 29751654 PMCid: PMC5982003. https://doi.org/10.3390/ijerph15050964
- Jin D, Bai Z, Chang D, Hoefel D, Jin B, Wang P, Wei D, Zhuang G. Biodegradation of di-n-butyl phthalatebyan isolated Gordonia sp. Strain QH-11. Journal of Genetic Identification and Degradation Kinetics. Hazard. Mater. 2012; 221-222:80–5. PMid: 22542774. https://doi.org/10.1016/j.jhazmat.2012.04.010
- Wang J, Liu P, Qian Y. Microbial degradation of di-n-butyl phthalate. Journal of Chemosphere. 1995; 31:4051–6. https://doi.org/10.1016/0045-6535(95)00282-D
- Wang J, Zhang M, Chen T, Zhu Y, Teng Y, Luo Y, Christie P. Isolation and identification of a Di-(2-Ethylhexyl) Phthalatedegrading bacterium and its role in the bioremediation of a contaminated soil. Pedosphere. 2015; 25:202–11. https://doi.org/10.1016/S1002-0160(15)60005-4
- Nahurira R, Ren L, Song J, Jia Y, Wang J, Fan S, Wang H, Yan Y. Degradation of Di(2-Ethylhexyl) phthalate by a novel gordonia alkanivorans strain YC-RL2. Curr Microbiol. 2017; 74:309–19.
- PMid: 28078431. https://doi.org/10.1007/s00284-016-1159-9
- Cortes-Lorenzo C, Sipkema D, Rodríguez-Diaz M, Fuentes S, Juarez-Jimenez B, Rodelas B, Smidt H, Gonzalez-Lopez. J. Microbial community dynamics in a submerged fixed bed bioreactor during biological treatment of saline urban wastewater. Ecol Eng. 2014; 71:126–32. https://doi.org/10.1016/j.ecoleng.2014.07.025
- Ren L, Lin Z, Liu H, Hu H. Bacteria-mediated phthalic acid esters degradation and related molecular mechanisms. Journal of Appl Microbiol Biotechnol. 2018; 102:1085–96. PMid: 29238874. https://doi.org/10.1007/s00253-017-8687-5
- Landon-Lane M. Corporate social responsibility in marine plastic debris governance. Journal of Mar Pollut Bull. 2018; 127:310–31. PMid: 29475667. https://doi.org/10.1016/j.marpolbul.2017.11.054
- He L, Fan S, Müller K, Wang H, Che L, Xu S, Song Z, Yuan G, Rinklebe J, Tsang DCW. Comparative analysis biochar and compost-induced degradation of di-(2-ethylhexyl) phthalate in soils. Journal of Sci Total Environ. 2018; 625:987–93. PMid: 29996465.