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Rashmi, Madhavi
- Potential of Bacillus cereus (Accession number KY7506901) on Di (2-Ethylhexyl) Phthalate Degradation and its Characterization by LCMS
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Authors
Affiliations
1 Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, IN
1 Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 20, No 1&2 (2020), Pagination: 114-119Abstract
The present work has been undertaken for remediating phthalate exposure in the environment. The microbial strain was isolated by enrichment culture technique from the rubbish dump space close to Patna that was contaminated with phthalates for higher degradation ability. The isolated microbial strain T7 was designated as Bacillus cereus after Gram-staining, biochemical characterization, 16S-rRNA sequence and phylogenetic studies. The isolate had the power to utilize 25O μg/ml Di (2-ethyl Hexyl Phthalate) (DEHP) dose taken from 10 mg/ml (DEHP) stock solution within the growth medium. The optimum pH and temperature for DEHP degradation were 8.5 at 37 ̊C. The isolated bacterial strain T7 may allow up to 10% NaCl in minimal salt medium that was enrich with DEHP. The metabolic end product obtained after LCMS was bis [3-(oxolan-2-yl) propyl] nonanedioate having chemical formula C23H40O6. This work provides some new proof for soil rectification by Bacillus species.
Keywords
Biochemical characterization, Biodegradation, DEHP, 16sr-RNA, LC-MS.Full Text
References
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- Biodegradation of Di-(2-Ethylhexyl Phalate) by Bacillus antracis (Accession no. KJ085972.1)
Abstract Views :237 |
PDF Views:99
Authors
Affiliations
1 TPS College, Patna – 800001, Bihar, IN
2 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, IN
3 DST-WOS, TPS College, Patna – 800001, Bihar, IN
1 TPS College, Patna – 800001, Bihar, IN
2 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, IN
3 DST-WOS, TPS College, Patna – 800001, Bihar, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 20, No 3&4 (2020), Pagination: 140-144Abstract
DEHP is one of the most broadly used PAEs (Phthalic Acid Esters) as a plasticizer in Polyvinyl Chloride (PVC) manufacturing. The DEHP and its other monoester metabolites are considered as very harmful for animal and human and affect the endocrine system. The strain isolated in this study is very efficient in degrading the DEHP. The degrading bacterial strain Bacillus anthracis is used in this study to determine the biological degradation potential of DEHP. Bacteria play very important roles in DEHP degradation in the environment under various conditions. The selected strain in for DEHP degrading bacteria designated as strain T-10 is optimized at the different conditions for its maximum activity at different temperatures, pH, chemicals like carbon and nitrogen source identified with degradation potential of more than 85%.
Keywords
Bacteria, Biodegradation, DEHP, PlasticizerFull Text
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