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Soni, Inderpal
- Evaluation of LD50 of Fenvalerate in Male Wistar Rats by Miller and Tainter Method
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Authors
Affiliations
1 Department of Zoology, Environmental Toxicological Laboratory, Centre for Advanced Studies, University of Rajasthan, Jaipur–302004, IN
2 Department of Zoology, Government Nehru Degree College, Ashoknagar, Madhya Pradesh, IN
1 Department of Zoology, Environmental Toxicological Laboratory, Centre for Advanced Studies, University of Rajasthan, Jaipur–302004, IN
2 Department of Zoology, Government Nehru Degree College, Ashoknagar, Madhya Pradesh, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 20, No 3&4 (2020), Pagination: 159-164Abstract
The principal aim of the present study was to evaluate median lethal dose (LD50) of fenvalerate, a synthetic pyrethroid (Type II) insecticide, in male Wistar rats. Median lethal dose (LD50) is defined as the dose which is lethal and proved to cause death in half of the experimental group of animals after specified test duration. The LD50 is a standardized measure for expressing and comparing the toxicity of chemicals. This test is proved to be an initial screening phase for the evaluation, measurement and comparing of the acute toxicity of a chemical. Fenvalerate dissolved in groundnut oil was administered to 5 different groups of animals (10 animals in each group) as a single dose by oral intubation. Based on the pilot study the selected doses of fenvalerate were 400,425,450,475 and 500mg/kg b.wt. for male rats. The animals were kept under observation for any type of toxic symptoms and death in 96 hours. The percentage of dead animals after 96 hours was calculated, which was then transformed into probits for the estimation of median lethal dose (LD50). In this study, the calculated median lethal dose (LD50) of fenvalerate dissolved in groundnut oil was found to be 434.51±29.90 mg/kg b.wt. in Wistar rats. In sub-chronic and chronic studies, this data is very helpful for the establishment pf the dosage regimen.
Keywords
Fenvalerate, LD50, Pyrethroid, Wistar RatsFull Text
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- Estimation of Median Lethal Dose of Fenpropathrin in Wistar Rat
Abstract Views :359 |
PDF Views:94
Authors
Affiliations
1 Environmental Toxicology Laboratory, Department of Zoology, University of Rajasthan, Jaipur – 302004, Rajasthan, IN
2 Assistant Professor and Head, Government Nehru PG College, Ashoknagar, Madhya Pradesh - 473331, IN
1 Environmental Toxicology Laboratory, Department of Zoology, University of Rajasthan, Jaipur – 302004, Rajasthan, IN
2 Assistant Professor and Head, Government Nehru PG College, Ashoknagar, Madhya Pradesh - 473331, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 21, No 1 (2021), Pagination: 16-22Abstract
Background: Fenpropathrin, a synthetic pyrethroid (Type I/II) is commonly used as an insecticide in homes and in agriculture. The present study was planned to determine the median lethal dose (LD50) of Fenpropathrin in adult Wistar rats, both male and female. Statistically, LD50 is a first screening step to asses and evaluate the toxicity for a chemical that causes death of 50% population of test animals when given by a specified route as a single dose for a specific time period. Methods: The experimental rats were divided into 10 groups (5 of male and 5 of female rats) and ten rats were divided into each group. For each group of animals, a single oral dose of Fenpropathrin dissolved in corn oil was administered orally at 15, 30, 45, 60 and 75 mg/kg body weight (bw) concentrations. The animals were monitored up to 96 hours to assess the signs of toxicity and to calculate the LD50 as per the graphical method procedure suggested by Miller and Tainter (1944)10. Result: Estimated LD50 of Fenpropathrin was found to be 52.72±8.61mg/kg body weight in male rats and 48.08±8.13 mg/kg body weight in female rats. There were no toxic signs or behavioural changes in the single oral dose of Fenpropathrin at 10mg/kg body weight, thus it can be considered as No Observed Adverse Effect Level (NOAEL). Conclusion: It can beconcluded from the study that Fenpropathrin is highly toxic pyrethroid due to its low LD50 value in Wistar rats.The result of this study may serve as a basis for dose administration for further research on Fenpropathrin toxicity.
Keywords
Fenpropathrin, Pyrethroid, Lethal Dose (LD50), Wistar RatFull Text
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