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DEHP is one of the most broadly used PAEs (Phthalic Acid Esters) as a plasticizer in Polyvinyl Chloride (PVC) manufacturing. The DEHP and its other monoester metabolites are considered as very harmful for animal and human and affect the endocrine system. The strain isolated in this study is very efficient in degrading the DEHP. The degrading bacterial strain Bacillus anthracis is used in this study to determine the biological degradation potential of DEHP. Bacteria play very important roles in DEHP degradation in the environment under various conditions. The selected strain in for DEHP degrading bacteria designated as strain T-10 is optimized at the different conditions for its maximum activity at different temperatures, pH, chemicals like carbon and nitrogen source identified with degradation potential of more than 85%.