Journal of Ecophysiology and Occupational Health

Open Access Open Access  Restricted Access Subscription Access

Biodegradation of Di-(2-Ethylhexyl Phalate) by Bacillus antracis (Accession no. KJ085972.1)


Affiliations
1 TPS College, Patna – 800001, Bihar, India
2 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, India
3 DST-WOS, TPS College, Patna – 800001, Bihar, India
 

DEHP is one of the most broadly used PAEs (Phthalic Acid Esters) as a plasticizer in Polyvinyl Chloride (PVC) manufacturing. The DEHP and its other monoester metabolites are considered as very harmful for animal and human and affect the endocrine system. The strain isolated in this study is very efficient in degrading the DEHP. The degrading bacterial strain Bacillus anthracis is used in this study to determine the biological degradation potential of DEHP. Bacteria play very important roles in DEHP degradation in the environment under various conditions. The selected strain in for DEHP degrading bacteria designated as strain T-10 is optimized at the different conditions for its maximum activity at different temperatures, pH, chemicals like carbon and nitrogen source identified with degradation potential of more than 85%.


Keywords

Bacteria, Biodegradation, DEHP, Plasticizer
User
Notifications
Font Size


  • Sant KE, Dolinoy DC, Jilek JL, Sartor MA, Harris C. Mono-2ethylhexyl phthalate disrupts neurulation and modifies the embryonic redox environment and gene expression. Journal of Reprod Toxicol. 2016; 63:32–48. PMid: 27167697 PMCid: PMC4987184. https://doi.org/10.1016/j.reprotox.2016.03.042

  • Nielsen E, Larsen PB. Toxicological evaluation and limit values for DEHP and phthalates other than DEHP. Environmental Review Report 6. Copenhagen, Denmark: Danish Environmental Protection Agency; 1996.

  • Ribbons DW, Keyser P, Kunz DA, Taylor BF. Microbial degradation of phthalates. Gibson, D.T., ed. Microbial degradation of organic compounds. New York: Dekker; 1984.

  • Sugatt RH, Ogrady DP, Banerjee S, Howard PH, Gledhill WE. Shake flask biodegradation of 14 commercial phthalate esters. Journal of Appl Environ Microbiol. 1984; 47:601–6. PMid: 16346500 PMCid: PMC239733. https://doi.org/10.1128/AEM.47.4.601-606.1984

  • Nozawa T, Maruyama Y. Anaerobic metabolism of phthalate and other aromatic-compounds by a denitrifying bacterium. Journal of Bacteriol. 1988; 170:5778–84. PMid: 3192515 PMCid: PMC211682. https://doi.org/10.1128/JB.170.12.57785784.1988

  • Staples CA, Peterson DR, Parkerton TF, Adams WJ. The environmental fate of phthalate esters: A literature review. Journal of Chemosphere. 1997; 35:667–749. https://doi.org/10.1016/S0045-6535(97)00195-1

  • Buchanna RE, Gibbons NE. Bergey’s manual of determinative bacteriology. 8th ed. Baltimore: Williams and Wilkins; 1984.

  • Tariq M. Isolation and screening of azo dye decolorizing bacterial isolates from dye contaminated textile wastewater. Journal of Soil Environ. 2011; 30(1):7–12.

  • Yang T, Ren L, Jia Y, Fan S, Wang J, Wang J, Nahurira R, Wang H, Yan Y. Biodegradation of Di-(2-ethylhexyl) Phthalate by Rhodococcus ruber YC-YT1 in Contaminated Water and Soil. Int Journal of Environ Res Public Health. 2018; 15(5):964. PMid: 29751654 PMCid: PMC5982003. https://doi.org/10.3390/ijerph15050964

  • Jin D, Bai Z, Chang D, Hoefel D, Jin B, Wang P, Wei D, Zhuang G. Biodegradation of di-n-butyl phthalatebyan isolated Gordonia sp. Strain QH-11. Journal of Genetic Identification and Degradation Kinetics. Hazard. Mater. 2012; 221-222:80–5. PMid: 22542774. https://doi.org/10.1016/j.jhazmat.2012.04.010

  • Wang J, Liu P, Qian Y. Microbial degradation of di-n-butyl phthalate. Journal of Chemosphere. 1995; 31:4051–6. https://doi.org/10.1016/0045-6535(95)00282-D

  • Wang J, Zhang M, Chen T, Zhu Y, Teng Y, Luo Y, Christie P. Isolation and identification of a Di-(2-Ethylhexyl) Phthalatedegrading bacterium and its role in the bioremediation of a contaminated soil. Pedosphere. 2015; 25:202–11. https://doi.org/10.1016/S1002-0160(15)60005-4

  • Nahurira R, Ren L, Song J, Jia Y, Wang J, Fan S, Wang H, Yan Y. Degradation of Di(2-Ethylhexyl) phthalate by a novel gordonia alkanivorans strain YC-RL2. Curr Microbiol. 2017; 74:309–19.

  • PMid: 28078431. https://doi.org/10.1007/s00284-016-1159-9

  • Cortes-Lorenzo C, Sipkema D, Rodríguez-Diaz M, Fuentes S, Juarez-Jimenez B, Rodelas B, Smidt H, Gonzalez-Lopez. J. Microbial community dynamics in a submerged fixed bed bioreactor during biological treatment of saline urban wastewater. Ecol Eng. 2014; 71:126–32. https://doi.org/10.1016/j.ecoleng.2014.07.025

  • Ren L, Lin Z, Liu H, Hu H. Bacteria-mediated phthalic acid esters degradation and related molecular mechanisms. Journal of Appl Microbiol Biotechnol. 2018; 102:1085–96. PMid: 29238874. https://doi.org/10.1007/s00253-017-8687-5

  • Landon-Lane M. Corporate social responsibility in marine plastic debris governance. Journal of Mar Pollut Bull. 2018; 127:310–31. PMid: 29475667. https://doi.org/10.1016/j.marpolbul.2017.11.054

  • He L, Fan S, Müller K, Wang H, Che L, Xu S, Song Z, Yuan G, Rinklebe J, Tsang DCW. Comparative analysis biochar and compost-induced degradation of di-(2-ethylhexyl) phthalate in soils. Journal of Sci Total Environ. 2018; 625:987–93. PMid: 29996465.


Abstract Views: 236

PDF Views: 99




  • Biodegradation of Di-(2-Ethylhexyl Phalate) by Bacillus antracis (Accession no. KJ085972.1)

Abstract Views: 236  |  PDF Views: 99

Authors

Tanuja Singh
TPS College, Patna – 800001, Bihar, India
Sonal Suman
Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, India
Madhavi Rashmi
Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, India
Anjali Singh
DST-WOS, TPS College, Patna – 800001, Bihar, India

Abstract


DEHP is one of the most broadly used PAEs (Phthalic Acid Esters) as a plasticizer in Polyvinyl Chloride (PVC) manufacturing. The DEHP and its other monoester metabolites are considered as very harmful for animal and human and affect the endocrine system. The strain isolated in this study is very efficient in degrading the DEHP. The degrading bacterial strain Bacillus anthracis is used in this study to determine the biological degradation potential of DEHP. Bacteria play very important roles in DEHP degradation in the environment under various conditions. The selected strain in for DEHP degrading bacteria designated as strain T-10 is optimized at the different conditions for its maximum activity at different temperatures, pH, chemicals like carbon and nitrogen source identified with degradation potential of more than 85%.


Keywords


Bacteria, Biodegradation, DEHP, Plasticizer

References





DOI: https://doi.org/10.18311/jeoh%2F2020%2F25041