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Potential of Bacillus cereus (Accession number KY7506901) on Di (2-Ethylhexyl) Phthalate Degradation and its Characterization by LCMS


Affiliations
1 Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, India
 

The present work has been undertaken for remediating phthalate exposure in the environment. The microbial strain was isolated by enrichment culture technique from the rubbish dump space close to Patna that was contaminated with phthalates for higher degradation ability. The isolated microbial strain T7 was designated as Bacillus cereus after Gram-staining, biochemical characterization, 16S-rRNA sequence and phylogenetic studies. The isolate had the power to utilize 25O μg/ml Di (2-ethyl Hexyl Phthalate) (DEHP) dose taken from 10 mg/ml (DEHP) stock solution within the growth medium. The optimum pH and temperature for DEHP degradation were 8.5 at 37 ̊C. The isolated bacterial strain T7 may allow up to 10% NaCl in minimal salt medium that was enrich with DEHP. The metabolic end product obtained after LCMS was bis [3-(oxolan-2-yl) propyl] nonanedioate having chemical formula C23H40O6. This work provides some new proof for soil rectification by Bacillus species.

 


Keywords

Biochemical characterization, Biodegradation, DEHP, 16sr-RNA, LC-MS.
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  • Potential of Bacillus cereus (Accession number KY7506901) on Di (2-Ethylhexyl) Phthalate Degradation and its Characterization by LCMS

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Authors

Madhavi Rashmi
Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, India
Sonal Suman
Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, India
Tanuja Singh
Department of Botany and Biotechnology, T.P.S. College, Patliputra University, Patna – 800020, Bihar, India

Abstract


The present work has been undertaken for remediating phthalate exposure in the environment. The microbial strain was isolated by enrichment culture technique from the rubbish dump space close to Patna that was contaminated with phthalates for higher degradation ability. The isolated microbial strain T7 was designated as Bacillus cereus after Gram-staining, biochemical characterization, 16S-rRNA sequence and phylogenetic studies. The isolate had the power to utilize 25O μg/ml Di (2-ethyl Hexyl Phthalate) (DEHP) dose taken from 10 mg/ml (DEHP) stock solution within the growth medium. The optimum pH and temperature for DEHP degradation were 8.5 at 37 ̊C. The isolated bacterial strain T7 may allow up to 10% NaCl in minimal salt medium that was enrich with DEHP. The metabolic end product obtained after LCMS was bis [3-(oxolan-2-yl) propyl] nonanedioate having chemical formula C23H40O6. This work provides some new proof for soil rectification by Bacillus species.

 


Keywords


Biochemical characterization, Biodegradation, DEHP, 16sr-RNA, LC-MS.

References





DOI: https://doi.org/10.18311/jeoh%2F2020%2F25087