Journal of Ecophysiology and Occupational Health

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Biodegradation of Pesticide Chlorpyrifos by Bacteria Staphylococcus aureus (Accession no. CP023500.1) Isolated from Agricultural Soil


Affiliations
1 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, India
2 Department of Biotechnology and Botany, TPS College, Patna – 800001, Bihar, India
3 Department of Biochemistry, Magadh University, Bodh Gaya – 824234, Bihar, India
4 Department of Zoology, Babasaheb Bhimrao Ambedkar Bihar University, Muzaffarapur – 842002, Bihar, India
 

The use of pesticides like Chlorpyrifos in agricultural soil is the primary reason for the pollution of aquatic and terrestrial environments. Today the most effective method used for bioremediation are by using microbes. Different pesticide degrading bacteria were isolated and identified by the mean of cultural, biochemical tests and which is further identified and confirmed by 16S RNA sequencing method. The most potent strain S-1 growth in mineral salt medium supplemented with Chlorpyrifos as sole source of carbon (50 to 1000 ug/ml) its optical density was measured at 600 nm. The bacterial growth is optimised on the parameter of different physiochemical condition were. The result showed that S. aureus shows maximum growth on 12th day. The HPLC analysis was also done for calculating the residual percentage of Chlorpyrifos after 12 days incubation which showed that S. aureus was able to degrade 99% of the pesticide of the 1000 ug/ml CP concentration in the MSM. The results of this research shows that the isolated bacteria have the potential to be used in bioremediation of Chlorpyrifos contaminated soil and water ecosystems.


Keywords

Bacterial, Bioremediation, Chlorpyrifos, Degradation, Pesticide.
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  • Biodegradation of Pesticide Chlorpyrifos by Bacteria Staphylococcus aureus (Accession no. CP023500.1) Isolated from Agricultural Soil

Abstract Views: 362  |  PDF Views: 195

Authors

Sonal Suman
Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, India
Tanuja Singh
Department of Biotechnology and Botany, TPS College, Patna – 800001, Bihar, India
Satyamvada Swayamprabha
Department of Biochemistry, Magadh University, Bodh Gaya – 824234, Bihar, India
Shivanand Singh
Department of Zoology, Babasaheb Bhimrao Ambedkar Bihar University, Muzaffarapur – 842002, Bihar, India

Abstract


The use of pesticides like Chlorpyrifos in agricultural soil is the primary reason for the pollution of aquatic and terrestrial environments. Today the most effective method used for bioremediation are by using microbes. Different pesticide degrading bacteria were isolated and identified by the mean of cultural, biochemical tests and which is further identified and confirmed by 16S RNA sequencing method. The most potent strain S-1 growth in mineral salt medium supplemented with Chlorpyrifos as sole source of carbon (50 to 1000 ug/ml) its optical density was measured at 600 nm. The bacterial growth is optimised on the parameter of different physiochemical condition were. The result showed that S. aureus shows maximum growth on 12th day. The HPLC analysis was also done for calculating the residual percentage of Chlorpyrifos after 12 days incubation which showed that S. aureus was able to degrade 99% of the pesticide of the 1000 ug/ml CP concentration in the MSM. The results of this research shows that the isolated bacteria have the potential to be used in bioremediation of Chlorpyrifos contaminated soil and water ecosystems.


Keywords


Bacterial, Bioremediation, Chlorpyrifos, Degradation, Pesticide.

References





DOI: https://doi.org/10.18311/jeoh%2F2020%2F25042