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Biodegradation of Pesticide Chlorpyrifos by Bacteria Staphylococcus aureus (Accession no. CP023500.1) Isolated from Agricultural Soil


Affiliations
1 Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, India
2 Department of Biotechnology and Botany, TPS College, Patna – 800001, Bihar, India
3 Department of Biochemistry, Magadh University, Bodh Gaya – 824234, Bihar, India
4 Department of Zoology, Babasaheb Bhimrao Ambedkar Bihar University, Muzaffarapur – 842002, Bihar, India
 

The use of pesticides like Chlorpyrifos in agricultural soil is the primary reason for the pollution of aquatic and terrestrial environments. Today the most effective method used for bioremediation are by using microbes. Different pesticide degrading bacteria were isolated and identified by the mean of cultural, biochemical tests and which is further identified and confirmed by 16S RNA sequencing method. The most potent strain S-1 growth in mineral salt medium supplemented with Chlorpyrifos as sole source of carbon (50 to 1000 ug/ml) its optical density was measured at 600 nm. The bacterial growth is optimised on the parameter of different physiochemical condition were. The result showed that S. aureus shows maximum growth on 12th day. The HPLC analysis was also done for calculating the residual percentage of Chlorpyrifos after 12 days incubation which showed that S. aureus was able to degrade 99% of the pesticide of the 1000 ug/ml CP concentration in the MSM. The results of this research shows that the isolated bacteria have the potential to be used in bioremediation of Chlorpyrifos contaminated soil and water ecosystems.


Keywords

Bacterial, Bioremediation, Chlorpyrifos, Degradation, Pesticide.
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  • Sachdeva S. Pesticides and their socio-economic impact on agriculture. South. Econ. 2007; 41(38):42–53. https://doi.org/10.1016/j.seps.2005.04.002
  • Fulekar MH. UGC major research project development of bioremediation technology for pesticide industrial wastes using novel cow dung microorganisms in sequence biological reactor and two phase partitioning bioreactor. 2008.
  • Chang TC, Chang HC, Yu FW, Leni D, Mario V, Chung T. Species level identification of isolates of the Acinetobacter calcoaceticus Acinetobacter baumanni complex by sequence analysis of the 16523SrRNA gene spacer region. J Clin Microbiol. 2005; 43(4):1632–9.PMid: 15814977 PMCid: PMC1081347. https://doi.org/10.1128/JCM.43.4.1632-1639.2005
  • Geetha M, Fulekar MH. Bioremediation of pesticides in surface soil treatment unit using microbial consortia. Afr J Environ Sci Technol. 2008; 2(2):36–45.
  • Zhu J, Zhao Y, Qiu J. Isolation and application of a Chlorpyrifosdegrading Bacillus licheniformis ZHU-1. Afr J Microbiol Res.2010; 4:2410–3.
  • Buchanna RE, Gibbons NE. Bergey’s manual of determinative bacteriology. 8th ed. Baltimore: Williams and Wilkins; 1984.
  • Aneja KR. Experiments in microbiology, plant pathology and biotechnology. Fourth ed. New Age International Limited. 2013.
  • Alexander M. Biodegradation and bioremediation. ed. 2 M.Alexander. San Diego: Academic Press; 1999.
  • Yang T, Ren L, Jia Y, Fan S, Wang J, Wang J, Nahurira R, Wang H, Yan Y. Biodegradation of Di-(2-ethylhexyl) Phthalate by Rhodococcus ruber YC-YT1 in contaminated water and soil.Int Journal of Environ Re. Public Health. 2018. p. 15–35. PMid: 29751654 PMCid: PMC5982003. https://doi.org/10.3390/ijerph15050964
  • Edgar RC. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res.2004; 32(5):1792–7. PMid: 15034147 PMCid: PMC390337.https://doi.org/10.1093/nar/gkh340
  • Talavera G, Castresana J. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology. 2007; 56:564–77.PMid: 17654362. https://doi.org/10.1080/10635150701472164
  • Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O. Phylogeny.f r: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008. p. 1:36. PMid: 18424797 PMCid: PMC2447785. https://doi.org/10.1093/nar/gkn180
  • Singh BK, Walker A, Morgan JAW, Wright DJ. Biodegradation of Chlorpyrifos by Enterobacter strain B-14 and its use in biodegradation of contaminated soils. Applied and Environmental Microbiology. 2004; 70:4855–63. PMid: 15294824 PMCid: PMC492451. https://doi.org/10.1128/AEM.70.8.4855-4863.2004
  • Vijayalakshmi P, Usha MS. Optimization of Chlorpyrifos degradation by Pseudomonas putida. J Chem Pharm. 2012; 4:2532–9.
  • Li X, He J, Li S. Isolation of Chlorpyrifos-degrading bacterium, Sphingomonas sp. strain Dsp-2 and cloning of the mpd gene.Research in Microbiology. 2007; 158:143–9. PMid: 17306510.https://doi.org/10.1016/j.resmic.2006.11.007
  • Fulekar MH. Bioremediation technologies for environment.Indian J Environ Protect. 2005; 25(4):358–64.
  • Rani SM, Lakshim KV, Devi SP, Madlian F, Aruna K. Isolation and characterization of Chlorpyrifos degrading bacteria from agricultural soil and its growth response. African Journal of Microbiology. 2008; 2:26–31.
  • Anwar S, Liaquat F, Khan QM, Khalid ZM, Iqbal S. Biodegradation of Chlorpyrifos and its hydrolysis product 3,5,6- trichloro-2pyridinol by Bacillus pumilus strain C2A1. J. Hazard. Mater.2009; 168(1):400–5. PMid: 19297093. https://doi.org/10.1016/j.jhazmat.2009.02.059

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  • Biodegradation of Pesticide Chlorpyrifos by Bacteria Staphylococcus aureus (Accession no. CP023500.1) Isolated from Agricultural Soil

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Authors

Sonal Suman
Department of Biotechnology, Magadh University, Bodh Gaya – 824234, Bihar, India
Tanuja Singh
Department of Biotechnology and Botany, TPS College, Patna – 800001, Bihar, India
Satyamvada Swayamprabha
Department of Biochemistry, Magadh University, Bodh Gaya – 824234, Bihar, India
Shivanand Singh
Department of Zoology, Babasaheb Bhimrao Ambedkar Bihar University, Muzaffarapur – 842002, Bihar, India

Abstract


The use of pesticides like Chlorpyrifos in agricultural soil is the primary reason for the pollution of aquatic and terrestrial environments. Today the most effective method used for bioremediation are by using microbes. Different pesticide degrading bacteria were isolated and identified by the mean of cultural, biochemical tests and which is further identified and confirmed by 16S RNA sequencing method. The most potent strain S-1 growth in mineral salt medium supplemented with Chlorpyrifos as sole source of carbon (50 to 1000 ug/ml) its optical density was measured at 600 nm. The bacterial growth is optimised on the parameter of different physiochemical condition were. The result showed that S. aureus shows maximum growth on 12th day. The HPLC analysis was also done for calculating the residual percentage of Chlorpyrifos after 12 days incubation which showed that S. aureus was able to degrade 99% of the pesticide of the 1000 ug/ml CP concentration in the MSM. The results of this research shows that the isolated bacteria have the potential to be used in bioremediation of Chlorpyrifos contaminated soil and water ecosystems.


Keywords


Bacterial, Bioremediation, Chlorpyrifos, Degradation, Pesticide.

References





DOI: https://doi.org/10.18311/jeoh%2F2020%2F25042