Journal of Ecophysiology and Occupational Health

Open Access Open Access  Restricted Access Subscription Access

Alkaline Phosphatase Activity as Indicator of Fecal Contamination of Mumbai Coastal Water


Affiliations
1 Department of Biotechnology, Thakur College of Science & Commerce, Kandivali, Mumbai, 400101, India
2 Department of Zoology, Thakur College of Science and Commerce, Kandivali, Mumbai, 400101, India
 

Mumbai coast is facing adverse impact of sewage discharge up to 5km from the shore. Microbiological quality of Mumbai coastal water was estimated by determination of faecal coliforms [FC], which is the standard used for assessment of level of fecal pollution of marine water. Determination of levels of faecal pollution of the coastal ecosystem can also be carried out by assessment of microbial metabolic activities in terms of alkaline phosphatase [APase] activity. High organic matter- allochthonous or autochthonous, or contaminants i.e. hydrocarbons support viability of bacterial and stimulates synthesis of enzymes to degrade new organic compounds. However AP activity is not only affected by various variables like pH, buffer ions, metal ion content, incubation time etc. but is also negatively correlated with availability of phosphates. Correlation between FC count and AP activity was studied to check use of AP activity as marker of ecological quality.

Keywords

Alkaline Phosphatase, Fecal Coliforms, Mumbai Coast, Sewage Contamination.
User
Notifications
Font Size


  • Blake, P.A., Weaver, R. and Hollis, D. (1981) Diseases of humans (other than cholera) caused by Vibrio. Annual review of Microbiology., 341–367.

  • Caruso, G., Denaro, R., Genovese, M., Giuliano, L., Mancuso, M., et al. (2004) New methodological strategies for detecting bacterial indicators. ChemEcol., 20, 167–181.

  • Denis, B., Robert, M., Frank, K., Mohamad, M. and Andreas, H.F. (2005) Discrimination efficacy of fecal pollution detection in different aquatic habitats of a high altitude tropical estuary, using presumptive coliforms, Escherichia coli and Clostridium perfringens spores. Applied And Environmental Microbiology., 65–71.

  • Engelbrecht, J.G. and Tredoux. (2004) Marine pollution indicators,(WISA).

  • Gabriella, C. (2013) Microbes and their use as Indicators of Pollution. Journal of Pollution effects and control.

  • Ivancic, I., et al. (2010) Phytoplankton and bacterial alkaline phosphatase activity in the northern Adriatic Sea. Marine Environmental Research., 69, 85–94.

  • Jane-Francis, T.K., Akoachere P.M.O., Beryl, S.M., Lucy, M.N., Gerald, N. and Roland, N.N. (2008) Bacterial indicators of pollution of the Douala lagoon, Cameroon: Public health implications. African Health Sciences., 85–89.

  • Jiang, S. (2001) Vibrio cholera in recreational beach waters and tributaries of Southern California. Hydrobiologia., 157–164.

  • Kimberly, A., John, E.W. and Valerie, J.H. (2005) Persistence and Differential Survival of Fecal Indicator Bacteria in Subtropical Waters and Sediments. Applied And Environmental Microbiology., 3041–3048.

  • Martinez, et al. (1993) Periplasmicaminopeptidase and alkalinephosphatase activities in a marine bacterium: Implications for substrate processing in the sea Biology Research Division, Scripps Institution of Oceanography, Marine Ecology Progress Seriesmar. Ecol. Prog. Ser., 92, 89–97.

  • Rozen, Y., et al. (2001) Survival of enteric bacteria in seawater. FEMS Microbiology Reviews., 25, 513–529.

  • Shamrao, A.I. and Arun, N.K. (2003) Pollution of some recreation beaches of mumbai, Maharashtra. Journal IAEM. 30, 172–175.

  • Shirodkar, S., Shirodkar, P.V., Rivonkar, S.D. and Pradhan, U.K. Evaluation of Physico-Chemical Characteristics of Water off Mumbai Coast using Exploratory Data Analysis, State Key Laboratory of Estuarine & Coastal Research Shanghai. UP state biodiversity board, international day for biodiversity.

  • Stewart, J.R., Gast, R.J., Fujioka, R.S., Solo-Gabriele, H.M., Meschke, J.S., et al. (2008) The coastal environment and human health: microbial indicators, pathogens, sentinels and reservoirs. Environ Health, S3.

  • Stojanovic, B.J. and Alexander, M. (1958). Effect of inorganic nitrogen on nitrification. Soil Science, 86, 208–215.

  • Sudhanandh, V.S., Udayakumar, P., Faisal, A.K., Potty, V.P., Ouseph, P.P., Prasanthan and Narendra, B.V.K. (2012) Distribution of potentially pathogenic enteric bacteria in coastal sea waters along the Southern Kerala coast, India. Journal of Environmental Biology., 61–66.


Abstract Views: 374

PDF Views: 156




  • Alkaline Phosphatase Activity as Indicator of Fecal Contamination of Mumbai Coastal Water

Abstract Views: 374  |  PDF Views: 156

Authors

Shubha Govil
Department of Biotechnology, Thakur College of Science & Commerce, Kandivali, Mumbai, 400101, India
Vitthal T. Mohite
Department of Zoology, Thakur College of Science and Commerce, Kandivali, Mumbai, 400101, India

Abstract


Mumbai coast is facing adverse impact of sewage discharge up to 5km from the shore. Microbiological quality of Mumbai coastal water was estimated by determination of faecal coliforms [FC], which is the standard used for assessment of level of fecal pollution of marine water. Determination of levels of faecal pollution of the coastal ecosystem can also be carried out by assessment of microbial metabolic activities in terms of alkaline phosphatase [APase] activity. High organic matter- allochthonous or autochthonous, or contaminants i.e. hydrocarbons support viability of bacterial and stimulates synthesis of enzymes to degrade new organic compounds. However AP activity is not only affected by various variables like pH, buffer ions, metal ion content, incubation time etc. but is also negatively correlated with availability of phosphates. Correlation between FC count and AP activity was studied to check use of AP activity as marker of ecological quality.

Keywords


Alkaline Phosphatase, Fecal Coliforms, Mumbai Coast, Sewage Contamination.

References





DOI: https://doi.org/10.15512/joeoh%2F2015%2Fv15i3-4%2F121587