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Receptor Mediated Effect of Melatonin in Pituitary Regulated Thyroid Secretions of Hyperthyroid Mice


Affiliations
1 Department of Zoology, Tripura University, Agartala – 799002, Tripura, India
 

The present study is designed to delineate the receptor mediated effect of melatonin in pituitary gland regulated thyroid secretions of hyperthyroid mice. Immuno histochemical study has been done to observe the melatonin receptors localization in pituitary gland. Western blot analysis was carried out to assess the expression patterns of melatonin receptors in pituitary gland and TSH receptors in thyroid gland. Hormonal analysis was done by ELISA kits. It was observed that introduction of melatonin reduced serum T3, T4, TSH hormones levels including thyroid TSH receptors those were fluctuated due to experimentally induced hyperthyroidism. The result has also shown the depletion of MT1 and MT2 receptors of pituitary gland due to melatonin treatment in hyperthyroidism. It may conclude that melatonin effects in pituitary regulated thyroid secretions by down regulation of pituitary MT1 and MT2 receptors in hyperthyroid condition.


Keywords

Melatonin, Melatonin Receptors, Pituitary, Thyroid Hormones, TSH Hormone, TSH Receptor.
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  • Baltaci AK, Mogulkoc R, Kul A, Bediz CS, Ugur A. Opposite effects of zinc and melatonin on thyroid hormones in rats. Toxicology. 2004; 195(1): 69–75. PMid: 14698569. https://doi.org/10.1016/j.tox.2003.09.001
  • Bubenik GA. Gastrointestinal melatonin: Localization, function and clinical relevance. Digestive Diseases and Sciences. 2002; 47(10):2336–48. PMid: 12395907. https://doi.org/10.1023/A:1020107915919
  • Gomez-Moreno G, Guardia J, Ferrera MJ, Cutando A, Reiter RJ. Melatonin in diseases of the oral cavity.
  • Oral Diseases. 2010; 16(3):242–7. PMid: 19682319. https://doi.org/10.1111/j.1601-0825.2009.01610.x
  • Johnston JD, Klosen P, Barrett P, Hazlerigg PG. Regulation of MT1 melatonin receptor expression in the foetal pituitary. Journal of Neuroendocrinology. 2006; 18(1):50–6. PMid: 16451220. https://doi.org/10.1111/j.13652826.2005.01389.x
  • Karbownik M, Lewinski A. The role of oxidative stress in physiological and pathological processes in the thyroid gland; possible involvement in pineal-thyroid interactions. Neuro Endocrinology Letters. 2003; 24(5):293–303.
  • Laskar P, Singh SS. Melatonin modulates thyroid hormones and splenocytes proliferation through mediation of its MT1 and MT2 receptors in hyperthyroid mice. Proceedings of the Zoological Society. 2018; 71(2):186–93. https://doi.org/10.1007/s12595-017-0244-9
  • Makay B, Makay O, Yenisey C, Icoz G, Ozgen G, Unsal E, Akyildiz M, Yetkin E. The interaction of oxidative stress response with cytokines in the thyrotoxic rat: Is there a link? Mediators of Inflammation. 2009; 2009:391682. PMid: 19343192 PMCid: PMC2662508. https://doi.org/10.1155/2009/391682
  • Masana MI, Doolen S, Ersahin C, Al-Ghoul WM, Duckles SP, Dubocovich ML, Krause DN. MT2 melatonin receptors are present and functional in rat caudal artery. Journal of Pharmacology and Experimental Therapeutics. 2002; 302(3):1295–302. PMid: 12183692. https://doi.org/10.1124/jpet.302.3.1295
  • Mogulkoc R, Baltaci AK. The effect of intraperitoneal melatonin supplementation on the release of thyroid hormones and testosterone in rats with hyperthyroid. Neuro Endocrinology Letters. 2003; 24(5):345–7.
  • Pandi-Perumal SR, Trakht I, Srinivasan V, Spence DW, Maestroni GJ, Zisapel N, Cardinali DP. Physiological effects of melatonin: Role of melatonin receptors and signal transduction pathways. Progress in Neurobiology. 2008; 85(3):335–53. PMid: 18571301. https://doi.org/10.1016/j.pneurobio.2008.04.001
  • Savaskan E, Wirz-Justice A, Olivieri G, Pache M, Krauchi K, Brydon L, Jokers R, Muller-Spahn F, Meyer P. Distribution of melatonin MT1 receptor immunoreactivity in human retina. Journal of Histochemistry and Cytochemistry. 2002; 50(4):519–26. PMid: 11897804. https://doi.org/10.1177/002215540205000408
  • Shinohara R, Mano T, Nagasaka A, Hayashi R, Uchimura K, Nakano I, Watanabe F, Tsugawa T, Makino M, Kakizawa H. Lipid peroxidation levels in rat cardiac muscle are affected by age and thyroid status. Journal of Endocrinology. 2000; 164(1):97–102. PMid: 10607942. https://doi.org/10.1677/joe.0.1640097
  • Siegrist C, Benedetti C, Orlando A. Lack of changes in serum prolactin, FSH, TSH and estradiol after melatonin treatment in doses that improve sleep and reduce benzodiazepine consumption in sleep-disturbed, middle-aged and elderly patients. Journal of Pineal Research. 2001; 30(1):34–42. PMid: 11168905. https://doi.org/10.1034/j.1600-079X.2001.300105.x
  • Slominski A, Tobin DJ, Zmijewski MA, Wortsman J, Paus R. Melatonin in the skin: Synthesis, metabolism and functions. Trends Endocrinology Metabolism. 2008; 19(1):17–24. PMid: 18155917. https://doi.org/10.1016/j.tem.2007.10.007
  • Tan DX, Manchester LC, Hardeland R, Lopez-Burillo S, Mayo JC, Sainz RM, Reiter RJ. Melatonin: A hormone, a tissue factor, an autocoid, a paracoid and an antioxidant vitamin. Journal of Pineal Research. 2003; 34(1):75–8. PMid: 12485375. https://doi.org/10.1034/j.1600-079X.2003.02111.x
  • Treeck O, Halder C, Ortmann O. Antiestrogens modulate MT1 melatonin receptor expression in breast and ovarian cancer cell lines. Oncology Reports. 2006; 15(1):231–5. PMid: 16328061. https://doi.org/10.3892/or.15.1.231
  • Wu YH, Ursinus J, Zhou JN, Frank AJL, Scheer,AJL, Bao A, Jockers R, Heerikhuize J, Swaab DF. Alterations of melatonin receptors MT1 and MT2 in the hypothalamic suprachiasmatic nucleus during depression. Journal of Affected Disorders. 2013; 148(2-3):357–67. PMid: 23357659. https://doi.org/10.1016/j.jad.2012.12.025

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  • Receptor Mediated Effect of Melatonin in Pituitary Regulated Thyroid Secretions of Hyperthyroid Mice

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Authors

Prashanjit Laskar
Department of Zoology, Tripura University, Agartala – 799002, Tripura, India
Shiv Shankar Singh
Department of Zoology, Tripura University, Agartala – 799002, Tripura, India

Abstract


The present study is designed to delineate the receptor mediated effect of melatonin in pituitary gland regulated thyroid secretions of hyperthyroid mice. Immuno histochemical study has been done to observe the melatonin receptors localization in pituitary gland. Western blot analysis was carried out to assess the expression patterns of melatonin receptors in pituitary gland and TSH receptors in thyroid gland. Hormonal analysis was done by ELISA kits. It was observed that introduction of melatonin reduced serum T3, T4, TSH hormones levels including thyroid TSH receptors those were fluctuated due to experimentally induced hyperthyroidism. The result has also shown the depletion of MT1 and MT2 receptors of pituitary gland due to melatonin treatment in hyperthyroidism. It may conclude that melatonin effects in pituitary regulated thyroid secretions by down regulation of pituitary MT1 and MT2 receptors in hyperthyroid condition.


Keywords


Melatonin, Melatonin Receptors, Pituitary, Thyroid Hormones, TSH Hormone, TSH Receptor.

References





DOI: https://doi.org/10.18311/jhsr%2F2020%2F25921