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Effect of Obestatin on Hepatic Injury Induced by Renal Ischemia/Reperfusion in Rat


Affiliations
1 Department of Physiology, Faculty of Medicine, Omar Al-Mukhtar University, Libya
2 Department of Biochemistry, Faculty of Nursing, Tobruk University, Libya
3 Department of Biochemistry, Faculty of Medicine, Benghazi University, Libya
4 Department of Biochemistry, Faculty of Medicine, Tobruk University, Libya
5 Department of Pathology, Tobruk Medical Center, Libya
6 Department of Physiology, Faculty of Medicine, Tanta University, Egypt
 

Background and Aims: Oxygen free radicals and cytokines are considered to be important components involved in the pathophysiological tissue alterations observed during Ischemia/Reperfusion (I/R). Based on the anti-oxidant and antiinflammatory effects of Obestatin (OB), we investigated the putative protective role of OB against I/R-induced oxidative remote organ injury. Materials and Methods: Male albino rats were subjected to either sham operation or bilateral renal artery clamping for 45 min and reperfusion for 24 h to induce I/R damage. Obestatin was administered intraperitoneally twice a day at a dose of 8 nmol/kg. At the end of the experimental procedure, the rats were decapitated and hepatic tissue were removed for biochemical analyses of: Malondialdehyde (MDA), an end product of lipid peroxidation; the activity of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT); the Myeloperoxidase (MPO) activity, as an indirect index of neutrophil infiltration and the level of pro-inflammatory cytokines (TNF-α and IL-1β). The serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Lactate Dehydrogenase (LDH) levels were measured to assess liver function and tissue damage, respectively. Pathological histology was also performed. Results: The results revealed the occurrence of I/R-induced oxidative organ damage, as confirmed histologically and evidenced by an increase in the MDA level and MPO activity, and a decrease in activity of SOD and CAT. Furthermore, serum AST, ALT, LDH levels, and tissue cytokines were elevated in the renal I/R group as compared to the sham operated control group. On the other hand, obestatin treatment succeeded to modulate these observed abnormalities resulting from I/R as indicated by the reduction of MAD and the pronounced improvement of the investigated biochemical and antioxidant parameters. Conclusion: Since obestatin administration reversed these oxidant responses, it seems likely that obestatin has a protective effect against oxidative organ damage induced by I/R.

Keywords

Obestatin (OB), Ischemia/Reperfusion (I/R), Oxidative Remote Organ Injury.
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  • Effect of Obestatin on Hepatic Injury Induced by Renal Ischemia/Reperfusion in Rat

Abstract Views: 336  |  PDF Views: 115

Authors

Aisha A. Muhammed
Department of Physiology, Faculty of Medicine, Omar Al-Mukhtar University, Libya
Bouzeed S. Boukhazeem
Department of Biochemistry, Faculty of Nursing, Tobruk University, Libya
Khlid G. Alqathafy
Department of Biochemistry, Faculty of Medicine, Benghazi University, Libya
Awad M. Alhasnony
Department of Biochemistry, Faculty of Medicine, Tobruk University, Libya
Ahmed G. Elsayed
Department of Pathology, Tobruk Medical Center, Libya
Elsayed A. Emara
Department of Physiology, Faculty of Medicine, Tanta University, Egypt

Abstract


Background and Aims: Oxygen free radicals and cytokines are considered to be important components involved in the pathophysiological tissue alterations observed during Ischemia/Reperfusion (I/R). Based on the anti-oxidant and antiinflammatory effects of Obestatin (OB), we investigated the putative protective role of OB against I/R-induced oxidative remote organ injury. Materials and Methods: Male albino rats were subjected to either sham operation or bilateral renal artery clamping for 45 min and reperfusion for 24 h to induce I/R damage. Obestatin was administered intraperitoneally twice a day at a dose of 8 nmol/kg. At the end of the experimental procedure, the rats were decapitated and hepatic tissue were removed for biochemical analyses of: Malondialdehyde (MDA), an end product of lipid peroxidation; the activity of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT); the Myeloperoxidase (MPO) activity, as an indirect index of neutrophil infiltration and the level of pro-inflammatory cytokines (TNF-α and IL-1β). The serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Lactate Dehydrogenase (LDH) levels were measured to assess liver function and tissue damage, respectively. Pathological histology was also performed. Results: The results revealed the occurrence of I/R-induced oxidative organ damage, as confirmed histologically and evidenced by an increase in the MDA level and MPO activity, and a decrease in activity of SOD and CAT. Furthermore, serum AST, ALT, LDH levels, and tissue cytokines were elevated in the renal I/R group as compared to the sham operated control group. On the other hand, obestatin treatment succeeded to modulate these observed abnormalities resulting from I/R as indicated by the reduction of MAD and the pronounced improvement of the investigated biochemical and antioxidant parameters. Conclusion: Since obestatin administration reversed these oxidant responses, it seems likely that obestatin has a protective effect against oxidative organ damage induced by I/R.

Keywords


Obestatin (OB), Ischemia/Reperfusion (I/R), Oxidative Remote Organ Injury.

References





DOI: https://doi.org/10.18311/jhsr%2F2020%2F24963