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Molecular Characterization of Adenovirus Causing Acute Respiratory Disease in Malaysia from 2003 to 2011


Affiliations
1 Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
 

Human Adenoviruses (HAdVs) are human pathogens that normally are associated with acute respiratory disease (ARD). Here, in this study, we characterized human adenovirus isolated from respiratory specimens collected from 2003 to 2011. Positive adenovirus were confirmed by cell culture and indirect immunoflourescence (IF) techniques. The isolates and positive clinical samples were subjected to adenovirus polymerase chain reaction, followed by DNA sequencing. BLAST searches and phylogenetic analysis revealed that only 2 species of HAdV, species C at 64% (47/73) and species B at 36% (26/73) were found circulating amongst ARD cases in Malaysia. Four types of HAdV species C were detected namely: HAdV-2 (66%), HAdV-1(17%), HAdV-5(13%) and HAdV-6(4%). For HAdV species B, only 2 types were detected with HAdV-3 being the highest at 58% and HAdV-7 at 42%. HadV-7 was associated with severe infections.

Keywords

Human Adenoviruses, Acute Respiratory Disease, Molecular Characterization, Gene Sequences.
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  • Molecular Characterization of Adenovirus Causing Acute Respiratory Disease in Malaysia from 2003 to 2011

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Authors

Y. Mohd Apandi
Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
M. Z. Zarina
Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
O. Khairul Azuan
Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
A. R. Nur Ismawati
Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
S. Zainah
Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia

Abstract


Human Adenoviruses (HAdVs) are human pathogens that normally are associated with acute respiratory disease (ARD). Here, in this study, we characterized human adenovirus isolated from respiratory specimens collected from 2003 to 2011. Positive adenovirus were confirmed by cell culture and indirect immunoflourescence (IF) techniques. The isolates and positive clinical samples were subjected to adenovirus polymerase chain reaction, followed by DNA sequencing. BLAST searches and phylogenetic analysis revealed that only 2 species of HAdV, species C at 64% (47/73) and species B at 36% (26/73) were found circulating amongst ARD cases in Malaysia. Four types of HAdV species C were detected namely: HAdV-2 (66%), HAdV-1(17%), HAdV-5(13%) and HAdV-6(4%). For HAdV species B, only 2 types were detected with HAdV-3 being the highest at 58% and HAdV-7 at 42%. HadV-7 was associated with severe infections.

Keywords


Human Adenoviruses, Acute Respiratory Disease, Molecular Characterization, Gene Sequences.