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Mukhopadhyay, Sarmishta

Functional Annotation of Pathogenesis Proteins in Shigella flexneri using Comparative Genomics

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Authors

Sarmishta Mukhopadhyay ¹, Sayak Ganguli ², Santanu Chakrabarti ¹

Affiliations
1 Department of Zoology, Government General Degree College, Singur, West Bengal, IN
2 Department of Biotechnology, St. Xavier’s College, Kolkata- 700016, IN

Source

Journal of Environment and Sociobiology, Vol 17, No 1 (2020), Pagination: 65-78

Abstract

The Gram-negative bacteria, Shigella species, is a predominant diarrheal pathogen and itself accounts for 15% of the diarrheal episodes occurring globally. Shigella enters the human body through ingestion of contaminated food and water and on reaching the intestine, dismantles the epithelial barrier, generating symptoms varying from mild to severe bloody diarrhea. Widespread diversity of Shigella species and the emergence of multi-drug resistant strains in recent years has made it extremely enigmatic to design a successful drug to combat shigellosis. This work focusses on comparative genomics methods to identify and annotate hypothetical proteins from the Shigella flexneri genome in quest of identifying novel druggable targets.

Keywords

Shigellosis, Hypothetical Proteins, Comparative Genomics, Drug Targets.

Full Text

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Insights Into The Structure And Dynamics Of Shigella Invasion Proteins For Use As Potential Drug Targets

Abstract Views :132 | PDF Views:0

Authors

Sarmishta Mukhopadhyay ¹, Sayak Ganguli ¹, Santanu Chakrabarti ²

Affiliations
1 Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata-700016, IN
2 Department of Zoology, Government General Degree College, Singur, West Bengal, IN

Source

Journal of Environment and Sociobiology, Vol 19, No 1 (2022), Pagination: 37-42

Abstract

The Gram-negative bacteria, Shigella spp. has always been a pathogen of concern in the context of global burden of diarrheal diseases, with recent surveys taking the annual incidence of shigellosis to roughly 125 million cases worldwide. The scenario has been further worsened by the frequent reports on isolation of multi drug resistant (MDR) Shigella strains, showing resistance against potent antibiotics including the 3rd generation cephalosporins as well. With the recent years witnessing a growing interest in the discovery of novel drugs, this work has scrutinized the structural nitty-gritty of the predominant virulence factors in Shigella, viz. the invasion plasmid antigens IpaA, IpaB, IpaC and IpaD, that are evidenced to have multifaceted role in the pathogenesis cascade, to explore their potential as promising therapeutic targets. In this work we report the 3D models of the different invasion proteins using comparative modeling and a comprehensive structural evaluation protocol for evaluating their stability as drug targets. Our data suggest significant potential of these invasion proteins to serve as future drug targets, thus opening up the avenue for further investigations.

Keywords

Shigella, Invasion proteins, Structural analysis, Drug targets, In-silico.

Full Text

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Predicting Drug Targets from Hypothetical Proteins of Pseudomonas Sp. Released from Permafrost Thawing under Impact of Climate Change

Abstract Views :126 | PDF Views:0

Authors

Arunima Bhattacharya ¹, Sarmishta Mukhopadhyay ¹, Sayak Ganguli ¹

Affiliations
1 Post-Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, West Bengal, IN

Source

Journal of Environment and Sociobiology, Vol 19, No 2 (2022), Pagination: 153-170

Abstract

One of the major consequences of the increase in global temperature is the thawing of permafrost, which is predicted to cause huge imbalances in natural ecosystems. The results of permafrost thawing is the resurface of quiescent psychrotolerant microbes which have been shown to be storehouses of antibiotic resistance genes (ARGs). Such superbugs, even if non-pathogenic, can transfer the ARGs to active pathogens, aggravating the existing public health crisis of antibiotic resistance. It is thus imperative to explore novel drug targets. Like most other organisms, bacteria possess coding sequences in the genome whose cellular and biochemical functions remain to be predicted. Functional annotation of such hypothetical proteins and their subsequent exploration as drug targets can thus be attempted as a novel computer-aided drug discovery approach. In this paper, we propose an in-silico pipeline for characterisation and functional annotation of hypothetical proteins using Pseudomonas aeruginosa, a multidrug-resistant WHO-listed critical priority pathogen. We then explore their potential as drug targets with small molecules of plant origin. Our results show considerable interactions between the proteins and the small molecules, including successful molecular docking, establishing a successful pipeline which may be useful in small molecule-based drug discovery in the near future.

Keywords

Permafrost thawing, Antibiotic resistance, Pseudomonas aeruginosa, Hypothetical protein

Full Text

References

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